The C-terminal domain of the heavy chain of tetanus toxin prevents the oxidative and nitrosative stress induced by acute toxicity of 1-methyl-4-phenylpyridinium, a rat model of Parkinson’s disease

Patricio, Felipe; Juárez-Torres, Daniel; Patricio-Martínez, Aleidy; Mendieta, Liliana; Pérez-Severiano, Francisca; Montes, Sergio; Aguilera, José; Limón, Ilhuicamina Daniel

doi:10.1016/j.neures.2021.08.005

Cited by 3 publications

(3 citation statements)

References 97 publications

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“…Tetanus toxin C fragment (TTC), a non-toxic protein component of tetanus toxin, retains immunological and neurobinding capabilities. 59 It can attach to the peripheral motor and sensory neurons and transfer them by axons to the spinal cord; thus, it is often employed in neuroanatomy retrograde tracing research. 60 Katherine L. Gibbs group established a real-time imaging and detection method for individual axonal transport in live mouse nerve fibers using fluorescently labelled and non-toxic fragment of tetanus neurotoxin (HcT) and analyzed the transit rate of retrograde HcT transport in axons after intramuscular and footpad injections, providing a new tool to analyze the specific mechanism of axonal transport cargo.…”

Section: Axonal-related Tracers or Antibodiesmentioning

confidence: 99%

Fluorescence imaging of peripheral nerve function and structure

Yang,

Zhang,

Liu

et al. 2023

J. Mater. Chem. B

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Section: Axonal-related Tracers or Antibodiesmentioning

confidence: 99%

Fluorescence imaging of peripheral nerve function and structure

Yang,

Zhang,

Liu

et al. 2023

J. Mater. Chem. B

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“…Bright-field immunohistochemistry for TH and GAD-67 was carried out on tissue sections taken from the striatum, Gpe and SNpc via the application of the previously-described freefloating, protocol (Apóstol Del Rosal et al, 2021;Patricio et al, 2022). The sections were washed three times in PBS-Triton X-100 at 0.2% for 10 min and then treated with 3% hydrogen peroxide (H 2 O 2 ) and 10% methanol to quench endogenous peroxidase activity.…”

Section: Tyrosine Hydroxylase and Glutamic Acid Decarboxylase (Gad-67...mentioning

confidence: 99%

Intrapallidal injection of cannabidiol or a selective GPR55 antagonist decreases motor asymmetry and improves fine motor skills in hemiparkinsonian rats

et al. 2022

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Cannabidiol (CBD) presents antiparkinsonian properties and neuromodulatory effects, possibly due to the pleiotropic activity caused at multiple molecular targets. Recently, the GPR55 receptor has emerged as a molecular target of CBD. Interestingly, GPR55 mRNA is expressed in the external globus pallidus (GPe) and striatum, hence, it has been suggested that its activity is linked to motor dysfunction in Parkinson’s disease (PD). The present study aimed to evaluate the effect of the intrapallidal injection of both CBD and a selective GPR55 antagonist (CID16020046) on motor asymmetry, fine motor skills, and GAD-67 expression in hemiparkinsonian rats. The hemiparkinsonian animal model applied involved the induction of a lesion in male Wistar rats via the infusion of the neurotoxin 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle via stereotaxic surgery. After a period of twenty days, a second surgical procedure was performed to implant a guide cannula into the GPe. Seven days later, lysophosphatidylinositol (LPI), CBD, or CID16020046 were injected once a day for three consecutive days (from the 28th to the 30th day post-lesion). Amphetamine-induced turning behavior was evaluated on the 14th and 30th days post-injury. The staircase test and fine motor skills were evaluated as follows: the rats were subject to a ten-day training period prior to the 6-OHDA injury; from the 15th to the 19th days post-lesion, the motor skills alterations were evaluated under basal conditions; and, from the 28th to the 30th day post-lesion, the pharmacological effects of the drugs administered were evaluated. The results obtained show that the administration of LPI or CBD generated lower levels of motor asymmetry in the turning behavior of hemiparkinsonian rats. It was also found that the injection of CBD or CID16020046, but not LPI, in the hemiparkinsonian rats generated significantly superior performance in the staircase test, in terms of the use of the forelimb contralateral to the 6-OHDA-induced lesion, when evaluated from the 28th to the 30th day post-lesion. Similar results were also observed for superior fine motor skills performance for pronation, grasp, and supination. Finally, the immunoreactivity levels were found to decrease for the GAD-67 enzyme in the striatum and the ipsilateral GPe of the rats injected with CBD and CID16020046, in contrast with those lesioned with 6-OHDA. The results obtained suggest that the inhibitory effects of CBD and CID16020046 on GPR55 in the GPe could be related to GABAergic overactivation in hemiparkinsonism, thus opening new perspectives to explain, at a cellular level, the reversal of the motor impairment observed in PD models.

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“…There is a clear criterion for PD model selection, but it needs to be further discussed in a PD non-neuroinflammatory study. The PD model includes toxin-based, gene-based, and synucleinbased models [7][8][9][10][11]. The toxin model imitates dopaminergic neurodegeneration by injecting neurotoxins, studying oxidative stress, mitochondrial respiratory defects, and abnormal protein aggregation among the three potentially important roles in PD pathogenesis [12,13].…”

Section: An Appropriate Model For Pd Neuroinflammatory Studymentioning

confidence: 99%

LncZFAS1 Inhibit MPP+-Induced Neuroinflammation Through TXNIP/MIB1 E3 Ubiquitin Ligase/NLRP3 Axis

2022

Journal of Cellular Immunology

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Neuroinflammation is associated with the occurrence and progression of Parkinson's disease (PD). Nucleotide-binding domain-like receptor protein-3 (NLRP3) is closely related to pyroptosis in PD-related cells and animal models, such as microglia and SH-SY5Y cells. The novel lncRNA ZFAS1 (LncZFAS1) regulates a variety of signaling pathways and participates in the inflammatory response in various diseases. However, their role in PD remains unclear. Our research found LncZFAS1 overexpression directly interfered with mir590-3p to inhibit thioredoxin-interacting protein (TXNIP) the Mindbomb 1 (MIB1) E3 ubiquitin ligase/NLRP3 pathway, which might reveal a new research approach to the mechanism of PD.

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The C-terminal domain of the heavy chain of tetanus toxin prevents the oxidative and nitrosative stress induced by acute toxicity of 1-methyl-4-phenylpyridinium, a rat model of Parkinson’s disease

Cited by 3 publications

References 97 publications

Fluorescence imaging of peripheral nerve function and structure

Fluorescence imaging of peripheral nerve function and structure

Intrapallidal injection of cannabidiol or a selective GPR55 antagonist decreases motor asymmetry and improves fine motor skills in hemiparkinsonian rats

LncZFAS1 Inhibit MPP+-Induced Neuroinflammation Through TXNIP/MIB1 E3 Ubiquitin Ligase/NLRP3 Axis

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