The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex

Cui, Xiaoyan; Yaghmaiean, Hoda; Wu, Guanwei; Wu, Xiaoyun; Chen, Xin; Thorn, G. D.; Wang, Aiming

doi:10.1016/j.virol.2017.07.016

Cited by 55 publications

(61 citation statements)

References 46 publications

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“…6B and shows that the GFP expressed from LONG(ΔP3N), like that from LONG(CI KS ) and in contrast with that from LONG, was unable to spread beyond the primary delivery area delimited by RFP. Taking into account previous reports proposing a key role of P3 in viral replication (33)(34)(35), this result suggests that the lack of the P3 N terminus prevents replication and supports the hypothesis that this defect impedes the assembly of CP in stable particles. With these results, however, we cannot rule out the possibility that the role of the P3 N terminus on virion assembly is independent of its participation during viral replication.…”

Section: Resultssupporting

confidence: 87%

A Functional Link between RNA Replication and Virion Assembly in the Potyvirus Plum Pox Virus

Gallo

Vallí

Calvo

et al. 2018

J Virol

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Accurate assembly of viral particles in the potyvirus (PPV) has been shown to depend on the contribution of the multifunctional viral protein HCPro. In this study, we show that other viral factors, in addition to the capsid protein (CP) and HCPro, are necessary for the formation of stable PPV virions. The CP produced in leaves from a subviral RNA termed LONG, which expresses a truncated polyprotein that lacks P1 and HCPro, together with HCPro supplied , was assembled into virus-like particles and remained stable after incubation. In contrast, deletions in multiple regions of the LONG coding sequence prevented the CP stabilization mediated by HCPro. In particular, we demonstrated that the first 178 amino acids of P3, but not a specific nucleotide sequence coding for them, are required for CP stability and proper assembly of PPV particles. Using a sequential coagroinfiltration assay, we observed that the subviral LONG RNA replicates and locally spreads in leaves expressing an RNA silencing suppressor. The analysis of the effect of both point and deletion mutations affecting RNA replication in LONG and full-length PPV demonstrated that this process is essential for the assembly of stable viral particles. Interestingly, in spite of this requirement, the CP produced by a nonreplicating viral RNA can be stably assembled into virions as long as it is coexpressed with a replication-proficient RNA. Altogether, these results highlight the importance of coupling encapsidation to other viral processes to secure a successful infection. Viruses of the family are among the most dangerous threats for basically every important crop, and such socioeconomical relevance has made them a subject of many research studies. In spite of this, very little is currently known about proteins and processes controlling viral genome encapsidation by the coat protein. In the case of (genus ), for instance, we have previously shown that the multitasking viral factor HCPro plays a role in the production of stable virions. Here, by using this potyvirus as a model, we move further to show that additional factors are also necessary for the efficient production of potyviral particles. More importantly, a comprehensive screening for such factors led us to the identification of a functional link between virus replication and packaging, unraveling a previously unknown connection of these two key events of the potyviral infection cycle.

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Section: Resultssupporting

confidence: 87%

A Functional Link between RNA Replication and Virion Assembly in the Potyvirus Plum Pox Virus

Gallo

Vallí

Calvo

et al. 2018

J Virol

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“…The SMV genome also harbours pipo (Chung et al, 2008;Wen and Hajimorad, 2010). The pipo of SMV, the first to be described in the movement of potyviruses, is expressed as a fusion protein (P3N 1 PIPO) as a consequence of transcriptional slippage (Chung et al, 2008;Cui et al, 2017;Olspert et al, 2015;Rodamilans et al, 2015;Vijayapalani et al, 2012;Wen and Hajimorad, 2010;White, 2015). SMV particles have served as a model to study the symmetry of flexuous viruses by a combination of cryo-electron microscopy, X-ray fibre diffraction and scanning transmission electron microscopy (Kendall et al, 2008).…”

Section: Genome Organization and Expressionmentioning

confidence: 99%

Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range

Hajimorad

Domier

Tolin

et al. 2018

Molecular Plant Pathology

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The interactions of SMV with soybean genotypes containing different dominant R genes and an understanding of the functional role(s) of SMV-encoded proteins in virulence, transmission and pathogenicity have been investigated intensively. The SMV-soybean pathosystem has become an excellent model for the examination of the genetics and genomics of a uniquely complex gene-for-gene resistance model in a crop of worldwide importance.

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“…Plant viruses have evolved different types of movement proteins (MPs) to modify PD gating properties to allow virion or viral nucleic acid cell-to-cell movement (Boevink & Oparka, 2005;Benitez-Alfonso et al, 2010;Ueki & Citovsky, 2011). For the Potyvirus members, accumulating evidence shows that P3N-PIPO functions as a MP to mediate viral cell-to-cell movement (Choi et al, 2005;Chung et al, 2008;Wei et al, 2010b;Wen & Hajimorad, 2010;Vijayapalani et al, 2012;Cui et al, 2017). P3N-PIPO recruits CI to PD (Wei et al, 2010b;Deng et al, 2015;Movahed et al, 2017), while CI serves as a docking point for the intercellular movement of virus replication vesicles (Movahed et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Remorin interacting with PCaP1 impairs Turnip mosaic virus intercellular movement but is antagonised by VPg

et al. 2019

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Group 1 Remorins (REMs) are extensively involved in virus trafficking through plasmodesmata (PD). However, their roles in Potyvirus cell-to-cell movement are not known. The plasma membrane (PM)-associated Ca 2+ binding protein 1 (PCaP1) interacts with the P3N-PIPO of Turnip mosaic virus (TuMV) and is required for TuMV cell-to-cell movement, but the underlying mechanism remains elusive.The mutant plants with overexpression or knockout of REM1.2 were used to investigate its role in TuMV cell-to-cell movement. Arabidopsis thaliana complementary mutants of pcap1 were used to investigate the role of PCaP1 in TuMV cell-to-cell movement. Yeast-two-hybrid, bimolecular fluorescence complementation, co-immunoprecipitation and RT-qPCR assays were employed to investigate the underlying molecular mechanism.The results show that TuMV-P3N-PIPO recruits PCaP1 to PD and the actin filament-severing activity of PCaP1 is required for TuMV intercellular movement. REM1.2 negatively regulates the cell-to-cell movement of TuMV via competition with PCaP1 for binding actin filaments. As a counteractive response, TuMV mediates REM1.2 degradation via both 26S ubiquitin-proteasome and autophagy pathways through the interaction of VPg with REM1.2 to establish systemic infection in Arabidopsis.This work unveils the actin cytoskeleton and PM nanodomain-associated molecular events underlying the cell-to-cell movement of potyviruses.

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The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex

Cited by 55 publications

References 46 publications

A Functional Link between RNA Replication and Virion Assembly in the Potyvirus Plum Pox Virus

A Functional Link between RNA Replication and Virion Assembly in the Potyvirus Plum Pox Virus

Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range

Remorin interacting with PCaP1 impairs Turnip mosaic virus intercellular movement but is antagonised by VPg

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