The C-terminal tail of the plant endosomal-type NHXs plays a key role in its function and stability

Cao, Boning; Xia, Zhongqiang; Hao, Zhanzhang; Liu, Changying; Long, Dan; Fan, Wei; Zhao, Aichun

doi:10.1016/j.plantsci.2020.110791

Cited by 4 publications

(3 citation statements)

References 49 publications

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“…All of the NMR spectra were collected on a Bruker AVANCE III 700 MHz spectrometer at Beijing NMR Center. The NMR raw data were processed based on the previously published protocol . MOL-MOL and PROCHECK_NMR software were applied to analyze the final P-2-CG structure.…”

Section: Methodsmentioning

confidence: 99%

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Anticoagulant Dodecapeptide Suppresses Thrombosis In Vivo by Inhibiting the Thrombin Exosite-I Binding Site

Cheng

Wang

Chen

et al. 2021

J. Agric. Food Chem.

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Thrombin is a crucial regulatory serine protease in hemostasis and thrombosis and has been a therapeutic target of thrombotic events. A novel oyster-derived thrombin inhibitory dodecapeptide (IEELEELEAER, P-2-CG) was identified and characterized. P-2-CG prolonged thrombin time from 9.6 s to 23.3 s at 5 mg/mL in vitro. P-2-CG bound to thrombin Exosite-I domain spontaneously. The occupied Exosite-I blocked fibrinogen binding, which prolonged fibrinogen clotting time to 28 s from 18.5 s. Molecule dynamics demonstrated the interaction of P-2-CG and thrombin Exosite-I involved in eight hydrogen bonds and lots of electrostatic forces. The residue Tyr 76 at thrombin Exosite-I is one critical amino acid for fibrinogen binding. The Glu 11 in P-2-CG was bound with Tyr 76 through strong hydrogen bonds and hydrophobic action. P-2-CG also significantly reduced the mortality of mice that suffered an acute pulmonary embolism induced by thrombin and inhibited mice tail thrombosis induced by κcarrageenan. The thrombin inhibitory efficiency in vitro and antithrombosis in vivo of P-2-CG provided insight for further applications to serve as an antithrombotic agent.

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Section: Methodsmentioning

confidence: 99%

“…The NMR raw data were processed based on the previously published protocol. 36 MOL-MOL and PROCHECK_NMR software were applied to analyze the final P-2-CG structure.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Anticoagulant Dodecapeptide Suppresses Thrombosis In Vivo by Inhibiting the Thrombin Exosite-I Binding Site

Cheng

Wang

Chen

et al. 2021

J. Agric. Food Chem.

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show abstract

“…In recent years, RNA-sequencing (RNA-seq) as an efficient and fast transcriptome research method has changed people's understanding of transcriptomics, and has been increasingly used to explore differences in gene expression in plants, such as licorice ( Glycyrrhiza glabra L.) 15 , luffa ( Luffa cylindrica (L.) Roem.) 16 , potato ( Solanum tuberosum L.) 17 , strawberry ( Fragaria ananassa Duch) 18 , and pear ( Pyrus betulifolia Bunge) 19 . In a study by Zhu et al .…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)

Liu

Zhang

Zhao³

et al. 2021

Sci Rep

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Fresh-cut processing promotes enzymatic browning of fresh fruits and vegetables, which negatively affects the product appearance and impacts their nutrition. We used RNA-sequencing to analyze the transcriptomic changes occurring during the browning of fresh-cut eggplant fruit samples from both browning-sensitive and browning-resistant cultivars to investigate the molecular mechanisms involved in browning. A total of 8347 differentially expressed genes were identified, of which 62 genes were from six gene families (i.e., PPO, PAL, POD, CAT, APX, and GST) potentially associated with enzymatic browning. Furthermore, using qRT-PCR, we verified 231 differentially regulated transcription factors in fresh-cut eggplant fruits. The enzyme activities of PPO, POD, PAL, and CAT in ‘36’ were significantly higher than those of ‘F’ fresh-cut for 15 min. Both PPO and POD play a major role in the browning of eggplant pulp and might therefore act synergistically in the browning process. Meanwhile, qPCR results of 18 browning related genes randomly screened in 15 eggplant materials with different browning tolerance showed variant-specific expression of genes. Lastly, gene regulatory networks were constructed to identify the browning-related genes. This work provides a basis for future molecular studies of eggplants, and lays a theoretical foundation for the development of browning-resistant fresh-cut fruits and vegetables.

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Regulation Effects of Na+/H+ Antiporter (NHX1) on Nicotiana tabacum Stressed with Metals of Different Valences

Wang

Da-yuan

Wang

et al. 2022

J Plant Growth Regul

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The C-terminal tail of the plant endosomal-type NHXs plays a key role in its function and stability

Cited by 4 publications

References 49 publications

Anticoagulant Dodecapeptide Suppresses Thrombosis In Vivo by Inhibiting the Thrombin Exosite-I Binding Site

Anticoagulant Dodecapeptide Suppresses Thrombosis In Vivo by Inhibiting the Thrombin Exosite-I Binding Site

Transcriptome profiling reveals potential genes involved in browning of fresh-cut eggplant (Solanum melongena L.)

Regulation Effects of Na+/H+ Antiporter (NHX1) on Nicotiana tabacum Stressed with Metals of Different Valences

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