The C‐terminus of the yeast Lsm4p is required for the association to P‐bodies

Mazzoni, Cristina; D’Addario, Irene; Falcone, Claudio

doi:10.1016/j.febslet.2007.09.009

Cited by 27 publications

(22 citation statements)

References 26 publications

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“…Interactions within prion-like domains of some RNP granule components might be important for formation of P-bodies and neuronal RNP granules (Decker et al, 2007;Mazzoni et al, 2007;Reijns et al, 2008;Salazar et al, 2010). Mechanisms involved in RNP granule assembly in the nematode germline include hydrophobic interactions between the phenylalanine-glycine repeat domains of VASA-related proteins GLH-1/2/4 (Updike et al, 2011), and self-interaction domains of P-granule abnormality family member (PGL) proteins (Hanazawa et al, 2011).…”

Section: Planarian Chromatoid Body Structurementioning

confidence: 98%

PRMT5 and the role of symmetrical dimethylarginine in chromatoid bodies of planarian stem cells

et al. 2012

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Planarian flatworms contain a population of adult stem cells (neoblasts) that proliferate and generate cells of all tissues during growth, regeneration and tissue homeostasis. A characteristic feature of neoblasts is the presence of chromatoid bodies, large cytoplasmic ribonucleoprotein (RNP) granules morphologically similar to structures present in the germline of many organisms. This study aims to reveal the function, and identify additional components, of planarian chromatoid bodies. We uncover the presence of symmetrical dimethylarginine (sDMA) on chromatoid body components and identify the ortholog of protein arginine methyltransferase PRMT5 as the enzyme responsible for sDMA modification in these proteins. RNA interference-mediated depletion of planarian PRMT5 results in defects in homeostasis and regeneration, reduced animal size, reduced number of neoblasts, fewer chromatoid bodies and increased levels of transposon and repetitive-element transcripts. Our results suggest that PIWI family member SMEDWI-3 is one sDMA-containing chromatoid body protein for which methylation depends on PRMT5. Additionally, we discover an RNA localized to chromatoid bodies, germinal histone H4. Our results reveal new components of chromatoid bodies and their function in planarian stem cells, and also support emerging studies indicative of sDMA function in stabilization of RNP granules and the Piwi-interacting RNA pathway.

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Section: Planarian Chromatoid Body Structurementioning

confidence: 98%

PRMT5 and the role of symmetrical dimethylarginine in chromatoid bodies of planarian stem cells

et al. 2012

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“…In addition, in metazoans, depletion of microscopically visible P-bodies does not seem to affect miRNA-mediated repression, decay of messages containing ARE elements, or decay of transcripts subject to NMD (Chu and Rana 2006;Stoecklin et al 2006;Eulalio et al 2007b). In contrast, deletion of the Lsm4 carboxy-terminal domain, which promotes Pbody assembly (Decker et al 2007;Mazzoni et al 2007;Reijns et al 2008), can affect mRNA degradation in at least some strains and/or conditions (Reijns et al 2008), which suggests that aggregation of individual mRNPs into larger structures may have some role in mRNA degradation. In addition, there is some evidence that P-body aggregation may play a role in the long-term survival of yeast cells during stationary phase (Ramachandran et al 2011).…”

Section: Why Do Mrnps Aggregate?mentioning

confidence: 99%

“…P-bodies are then formed from these individual mRNPs aggregating into larger structures. In yeast, aggregation of mRNPs into P-bodies has been shown to be primarily dependent on a self-interaction domain (referred to as a Yjef-N domain) in the Edc3 protein and a glutamine/asparagine (Q/N) rich prion-like domain in the Lsm4 carboxyl terminus (Decker et al 2007;Mazzoni et al 2007;Reijns et al 2008). Because the YjeF domain of Edc3 is conserved (Ling et al 2008), it is likely that Edc3 will contribute to assembly of metazoan Pbodies.…”

Section: How Do Cytoplasmic Mrnp Granules Assemble?mentioning

confidence: 99%

P-Bodies and Stress Granules: Possible Roles in the Control of Translation and mRNA Degradation

Decker

Parker

2012

Cold Spring Harbor Perspectives in Biology

697

665

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The control of translation and mRNA degradation is important in the regulation of eukaryotic gene expression. In general, translation and steps in the major pathway of mRNA decay are in competition with each other. mRNAs that are not engaged in translation can aggregate into cytoplasmic mRNP granules referred to as processing bodies (P-bodies) and stress granules, which are related to mRNP particles that control translation in early development and neurons. Analyses of P-bodies and stress granules suggest a dynamic process, referred to as the mRNA Cycle, wherein mRNPs can move between polysomes, P-bodies and stress granules although the functional roles of mRNPassembly into higher order structures remain poorly understood. In this article, we review what is known about the coupling of translation and mRNA degradation, the properties of P-bodies and stress granules, and how assembly of mRNPs into larger structures might influence cellular function.

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“…This may be explained by the targeting of mRNAs to stress granules, which are thought to act as storage factors that sort mRNAs for degradation or later translation following the relief of stress conditions (Teixeira et al 2005). Storage or degradation of mRNAs in P bodies and stress granules may play an important role in regulating gene expression during oxidative stress conditions since it has been shown that the number of P bodies is significantly increased following UV and hydrogen peroxide stress (Teixeira et al 2005;Mazzoni et al 2007). …”

Section: Cellular Responses To Rosmentioning

confidence: 99%

The Response to Heat Shock and Oxidative Stress inSaccharomyces cerevisiae

2012

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A common need for microbial cells is the ability to respond to potentially toxic environmental insults. Here we review the progress in understanding the response of the yeast Saccharomyces cerevisiae to two important environmental stresses: heat shock and oxidative stress. Both of these stresses are fundamental challenges that microbes of all types will experience. The study of these environmental stress responses in S. cerevisiae has illuminated many of the features now viewed as central to our understanding of eukaryotic cell biology. Transcriptional activation plays an important role in driving the multifaceted reaction to elevated temperature and levels of reactive oxygen species. Advances provided by the development of whole genome analyses have led to an appreciation of the global reorganization of gene expression and its integration between different stress regimens. While the precise nature of the signal eliciting the heat shock response remains elusive, recent progress in the understanding of induction of the oxidative stress response is summarized here. Although these stress conditions represent ancient challenges to S. cerevisiae and other microbes, much remains to be learned about the mechanisms dedicated to dealing with these environmental parameters.

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The C‐terminus of the yeast Lsm4p is required for the association to P‐bodies

Cited by 27 publications

References 26 publications

PRMT5 and the role of symmetrical dimethylarginine in chromatoid bodies of planarian stem cells

PRMT5 and the role of symmetrical dimethylarginine in chromatoid bodies of planarian stem cells

P-Bodies and Stress Granules: Possible Roles in the Control of Translation and mRNA Degradation

The Response to Heat Shock and Oxidative Stress inSaccharomyces cerevisiae

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