The Caco-2 cell culture model enables sensitive detection of enhanced protein permeability in the presence of N-decyl-β-d-maltopyranoside

Marušič, Maja; Zupančič, Tina; Hribar, Gorazd; Komel, Radovan; Anderluh, Gregor; Caserman, Simon

doi:10.1016/j.nbt.2013.05.008

Cited by 8 publications

(7 citation statements)

References 26 publications

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“…The mean basal TEER value for Caco-2 monolayers was 1980 ± 104 Ω cm 2 (n=36), within 236 the range reported by this lab [29] and others [38]. The addition of Gly-Sar had no effect on 237…”

Section: Transport Of [ 3 H]-ipp [ 3 H]-lkp and [ 14 C]-mannitol Acrsupporting

confidence: 79%

Evaluation of PepT1 transport of food-derived antihypertensive peptides, Ile-Pro-Pro and Leu-Lys-Pro using in vitro, ex vivo and in vivo transport models

Gleeson

Brayden

Ryan

2017

European Journal of Pharmaceutics and Biopharmaceutics

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Publication informationEuropean Journal of Pharmaceutics and Biopharmaceutics, Publisher ElsevierItem record/more information http://hdl.handle.net/10197/8514 an apical buffer pH of 7.4. Gly-Sar reduced the P app across isolated jejunal mucosae and the 32 area under the curve (AUC) in intra-jejunal instillations when the apical/luminal buffer pH 33 was either 7.4 or 6.5. However, the jejunal surface acidic pH was maintained in rat jejunal 34 tissue even when the apical side buffer pH was 7.4 due to the presence of the microclimate 35 which is not present in monolayers. PepT1 expression was confirmed by 36 immunofluorescence on monolayers and brush border of rat jejunal tissue. This data suggest 37 that IPP and LKP are highly permeable and cross small intestinal epithelia in part by the 38 PepT1 transporter, with an additional contribution from the paracellular route. 39 40 41 Publisher's statement þÿ T h i s i s t h e a u t h o r s v e r s i o n o f a w o r k t h a t w a s a c c e p t e d f o r p u b l i c a t i o n i n E u r o p e a n

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Section: Transport Of [ 3 H]-ipp [ 3 H]-lkp and [ 14 C]-mannitol Acrsupporting

confidence: 79%

Evaluation of PepT1 transport of food-derived antihypertensive peptides, Ile-Pro-Pro and Leu-Lys-Pro using in vitro, ex vivo and in vivo transport models

Gleeson

Brayden

Ryan

2017

European Journal of Pharmaceutics and Biopharmaceutics

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“…For example, membrane perturbations by saponines have been shown to cause a drop in TEER and cytoskeletal changes that result in disruption of tight junction proteins and subsequent increase in paracellular permeability [54]. TEER values of Caco-2 cells used in our experiments ranged from 2 to 3 kΩ*cm 2 , which indicated that Caco-2 cell monolayer was mature and intact [42]. Caco-2 cells were treated with increasing concentrations of LLO.…”

Section: Resultsmentioning

confidence: 99%

“…Since we noted the effect of LLO and EqtII on these structures, we wanted to see if this correlates with an increase of paracellular permeability to certain molecules. Also, Caco-2 cell monolayers have been widely used to assess the transepithelial movements of various substances like drugs, amino acids and metal ions [40–42,78,79]. If LLO or EqtII could accelerate the passage of certain molecules across the intestinal barrier without compromising the integrity of the epithelium, they could potentially be used to improve absorption of orally administered drugs in the intestinal tract.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, we examined the effects of LLO on the integrity of the Caco-2 epithelium. Caco-2 cell line is derived from a colorectal adenocarcinoma and exhibits several morphological and biochemical characteristics of small intestinal enterocytes and is often used for permeability studies of various molecules [40–42]. For comparison we used Equinatoxin II (EqtII), a pore-forming toxin from the sea anemone Actinia equina , in order to distinguish between effects due to pore-formation or any other LLO-specific effects on Caco-2 cells.…”

Section: Introductionmentioning

confidence: 99%

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Listeriolysin O Affects the Permeability of Caco-2 Monolayer in a Pore-Dependent and Ca2+-Independent Manner

et al. 2015

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Listeria monocytogenes is a food and soil-borne pathogen that secretes a pore-forming toxin listeriolysin O (LLO) as its major virulence factor. We tested the effects of LLO on an intestinal epithelial cell line Caco-2 and compared them to an unrelated pore-forming toxin equinatoxin II (EqtII). Results showed that apical application of both toxins causes a significant drop in transepithelial electrical resistance (TEER), with higher LLO concentrations or prolonged exposure time needed to achieve the same magnitude of response than with EqtII. The drop in TEER was due to pore formation and coincided with rearrangement of claudin-1 within tight junctions and associated actin cytoskeleton; however, no significant increase in permeability to fluorescein or 3 kDa FITC-dextran was observed. Influx of calcium after pore formation affected the magnitude of the drop in TEER. Both toxins exhibit similar effects on epithelium morphology and physiology. Importantly, LLO action upon the membrane is much slower and results in compromised epithelium on a longer time scale at lower concentrations than EqtII. This could favor listerial invasion in hosts resistant to E-cadherin related infection.

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“…It is a glycoprotein hormone, which is the principal regulator of red blood cell formation (23). Due to the required high doses of parenterally administered EPO, severe side effects have been reported for cancer patients taking EPO and receiving chemotherapy and/or radiation therapy (24). Therefore, there is an unmet need to develop new and improved EPO delivery systems for its oral administration, which might then allow low-dose long-term therapy.…”

mentioning

confidence: 99%

Effect of surface hydrophobicity of therapeutic protein loaded in polyelectrolyte nanoparticles on transepithelial permeability

Miklavžin

Cegnar²,

Kerč

et al. 2018

Acta Pharmaceutica

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Oral delivery of protein drugs is greatly limited by low hydrophobicity, an important determinant for intestinal epithelial permeation and bioavailability. Herein, surface properties of recombinant erythropoietin were investigated using the fluorescent dye bis-ANS to monitor relative hydrophobicity for correlation with permeabilities with Caco-2 cells. At various pHs, bis-ANS fluorescence intensity indicated different surface hydrophobicities of erythropoietin molecules. Erythropoietin incorporated in chitosan or chitosan-trimethylchitosan (CS-TMC) nanoparticles prepared by polyelectrolyte complexation and ionotropic gelation with tripolyphosphate also showed different surface hydrophobicities. Chitosan nanoparticles with erythropoietin provided the most hydrophobic surface, followed by free erythropoietin (in water) and that loaded into CS-TMC nanoparticles. Chitosan nanoparticles were more effective than CS-TMC nanoparticles for permeation of erythropoietin across Caco-2 cell monolayers; the lowest permeability was shown by erythropoietin itself. Thus, hydrophilic protein molecules complexed with polyelectrolytes can provide more hydrophobic surfaces that enhance transepithelial permeability. This bis-ANS method also provides valuable information for the design of polyelectrolyte nanoparticules for oral delivery of protein drugs.

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The Caco-2 cell culture model enables sensitive detection of enhanced protein permeability in the presence of N-decyl-β-d-maltopyranoside

Cited by 8 publications

References 26 publications

Evaluation of PepT1 transport of food-derived antihypertensive peptides, Ile-Pro-Pro and Leu-Lys-Pro using in vitro, ex vivo and in vivo transport models

Evaluation of PepT1 transport of food-derived antihypertensive peptides, Ile-Pro-Pro and Leu-Lys-Pro using in vitro, ex vivo and in vivo transport models

Listeriolysin O Affects the Permeability of Caco-2 Monolayer in a Pore-Dependent and Ca2+-Independent Manner

Effect of surface hydrophobicity of therapeutic protein loaded in polyelectrolyte nanoparticles on transepithelial permeability

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