The Calcitonin/Calcitonin Gene-Related Peptide Family in Invertebrate Deuterostomes

Sekiguchi, Toshio

doi:10.3389/fendo.2018.00695

Cited by 17 publications

(5 citation statements)

References 76 publications

(162 reference statements)

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“…Their work is the first molecular and functional characterization of a CT/CGRP family receptor and of RAMPs from invertebrates ( Sekiguchi et al, 2016 ). Two reviews by Sekiguchi provide a summary of the CT/CGRP family peptides and their receptors in mammals and invertebrate deuterostomes, highlighting teleosts, urochordates, cephalochordates, and invertebrate chordates, including ascidians and amphioxi ( Sekiguchi, 2018 , 2022 ). Moreover, putative CT/CGRP family peptides are identified in cartilaginous fish based on genomic data analysis.…”

Section: Introductionmentioning

confidence: 99%

Elucidating the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins

et al. 2022

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G protein-coupled receptors (GPCRs) are known to interact with several other classes of integral membrane proteins that modulate their biology and pharmacology. However, the extent of these interactions and the mechanisms of their effects are not well understood. For example, one class of GPCR-interacting proteins, receptor activity-modifying proteins (RAMPs), comprise three related and ubiquitously expressed single-transmembrane span proteins. The RAMP family was discovered more than two decades ago, and since then GPCR–RAMP interactions and their functional consequences on receptor trafficking and ligand selectivity have been documented for several secretin (class B) GPCRs, most notably the calcitonin receptor-like receptor. Recent bioinformatics and multiplexed experimental studies suggest that GPCR–RAMP interactions might be much more widespread than previously anticipated. Recently, cryo-electron microscopy has provided high-resolution structures of GPCR–RAMP–ligand complexes, and drugs have been developed that target GPCR–RAMP complexes. In this review, we provide a summary of recent advances in techniques that allow the discovery of GPCR–RAMP interactions and their functional consequences and highlight prospects for future advances. We also provide an up-to-date list of reported GPCR–RAMP interactions based on a review of the current literature. Significance Statement Receptor activity-modifying proteins (RAMPs) have emerged as modulators of many aspects of G protein-coupled receptor (GPCR)biology and pharmacology. The application of new methodologies to study membrane protein–protein interactions suggests that RAMPs interact with many more GPCRs than had been previously known. These findings, especially when combined with structural studies of membrane protein complexes, have significant implications for advancing GPCR-targeted drug discovery and the understanding of GPCR pharmacology, biology, and regulation.

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Section: Introductionmentioning

confidence: 99%

Elucidating the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins

et al. 2022

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“…Pharyngeal bars are also supported internally by a skeletal rod, consis.ng of an expanded acellular collagenous matrix 56 . Cephalochordates have three calcitonin genes, called CTFPs (calcitonin family proteins), that are expressed in different combina.ons in neurons of the amphioxus larva [57][58][59] . We iden.fied the complete sequence and genomic loca.on of the three CTFP genes in Branchiostoma lanceolatum.…”

Section: Iden;fica;on Of Puta;ve Endoderm-derived C-cells In Non-vert...mentioning

confidence: 99%

A pre-vertebrate endodermal origin of calcitonin-producing neuroendocrine cells

Rees,

Kirk,

Gattoni

et al. 2024

Preprint

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SummaryVertebrate calcitonin-producing cells (C-cells) are neuroendocrine cells that secrete the small peptide hormone calcitonin in response to elevated blood calcium levels. C-cells are crucial for maintenance of calcium homeostasis, yet the embryonic and evolutionary origins of this cell type remain contentious. Whereas mouse C-cells reside within the thyroid gland and derive from pharyngeal endoderm, avian C-cells are located within ultimobranchial glands and were reported to derive from the neural crest. We use a comparative cell lineage tracing approach in a range of vertebrate model systems to resolve the ancestral embryonic origin of vertebrate C-cells. We find, contrary to previous studies, that chick C-cells derive from pharyngeal endoderm, with neural crest-derived cells instead contributing to connective tissue intimately associated with C-cells in the ultimobranchial gland. This endodermal origin of C-cells is conserved in a ray-finned bony fish (zebrafish) and a cartilaginous fish (the little skate,Leucoraja erinacea). Furthermore, we discover putative C-cell homologues within the endodermally-derived pharyngeal epithelium of the ascidianCiona intestinalisand the amphioxusBranchiostoma lanceolatum, two invertebrate chordates that lack neural crest cells. Our findings point to a conserved endodermal origin of C-cells across vertebrates and to a pre-vertebrate origin of this cell type along the chordate stem.

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“…This prompted us to look further into putative neurosecretory centers in the amphioxus brain. We investigated the distribution of the three amphioxus neuropeptides of the calcitonin/CGRP family (calcitonin family proteins, CTFPs) 91,92 . In 1gs larvae, CTFP3 is indeed expressed in the anterior cerebral vesicle within the miR-7 domain (Figure 6B), while CTFP1 is restricted to the post-infundibular region and CTFP2 is expressed more posteriorly.…”

Section: Apical Patterning Is Essential To Set the Hypothalamic Ident...mentioning

confidence: 99%

An ancient gene regulatory network sets the position of the forebrain in chordates

Gattoni

Keitley

Sawle

et al. 2023

Preprint

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The evolutionary origin of the vertebrate brain is still a major subject of debate. Its distinctive dorsal position and development from a tubular neuroepithelium are unique to the chordate phylum. Conversely, apical organs (AO) are larval sensory/neurosecretory centers found in many invertebrate taxa, including in animals without a brain. Previous studies have shown that AOs are specified by a conserved set of genes under the influence of Wnt signalling. Although most of these genes are expressed in chordate nervous systems (including vertebrates), no AOs have ever been described in this group of animals. Here we have exploited single-cell genomic approaches to characterize cells showing AO profiles in sea urchin (ambulacrarian), amphioxus (invertebrate chordate) and zebrafish (vertebrate chordate). This, in combination with co-expression analysis in amphioxus embryos, has allowed us to identify an active and dynamic anterior Gene Regulatory Network (aGRN) in the three deuterostome species. We have further discovered that as well as controlling AO specification in sea urchin, this aGRN is involved in the formation of the hypothalamic region in amphioxus and zebrafish. Using a functional approach, we find that the aGRN is controlled by Wnt signalling in amphioxus, and that suppression of the aGRN by Wnt overactivation leads to a loss of forebrain cell types. The loss of the forebrain does not equate to a reduction of neuronal tissue, but to a loss of identity, suggesting a new role for Wnt in amphioxus in specifically positioning the forebrain. We thus propose that the aGRN is conserved throughout bilaterians and that in the chordate lineage was incorporated into the process of neurulation to position the brain, thereby linking the evolution of the AO to that of the chordate forebrain.

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The Calcitonin/Calcitonin Gene-Related Peptide Family in Invertebrate Deuterostomes

Cited by 17 publications

References 76 publications

Elucidating the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins

Elucidating the Interactome of G Protein-Coupled Receptors and Receptor Activity-Modifying Proteins

A pre-vertebrate endodermal origin of calcitonin-producing neuroendocrine cells

An ancient gene regulatory network sets the position of the forebrain in chordates

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