The cAMP receptor protein (CRP) enhances the competitive nature of Salmonella Typhimurium

Sawant, Kirti; Shashidhar, Ravindranath

doi:10.1007/s00203-023-03528-6

Cited by 3 publications

(1 citation statement)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absence of crp made the SCCF01 lose the metabolic ability to use maltose, mannitol, and trehalose as carbon sources; the deletion can only use glucose as its sole carbon source. A recent study has also shown that the CRP protein is involved in assessing energy balance within cells and is essential for efficient nutrient assimilation in a competitive environment [36]. Overall, the aspects mentioned above made the ∆crp grow slowly.…”

Section: Discussionmentioning

confidence: 99%

The cAMP Receptor Protein (CRP) of Vibrio mimicus Regulates Its Bacterial Growth, Type II Secretion System, Flagellum Formation, Adhesion Genes, and Virulence

Tian,

Xiang,

Peng

et al. 2024

Animals

View full text Add to dashboard Cite

Vibrio mimicus is a serious pathogen in aquatic animals, resulting in significant economic losses. The cAMP receptor protein (CRP) often acts as a central regulator in highly pathogenic pathogens. V. mimicus SCCF01 is a highly pathogenic strain isolated from yellow catfish; the crp gene deletion strain (Δcrp) was constructed by natural transformation to determine whether this deletion affects the virulence phenotypes. Their potential molecular connections were revealed by qRT-PCR analysis. Our results showed that the absence of the crp gene resulted in bacterial and colony morphological changes alongside decreases in bacterial growth, hemolytic activity, biofilm formation, enzymatic activity, motility, and cell adhesion. A cell cytotoxicity assay and animal experiments confirmed that crp contributes to V. mimicus pathogenicity, as the LD50 of the Δcrp strain was 73.1-fold lower compared to the WT strain. Moreover, qRT-PCR analysis revealed the inhibition of type II secretion system genes, flagellum genes, adhesion genes, and metalloproteinase genes in the deletion strain. This resulted in the virulence phenotype differences described above. Together, these data demonstrate that the crp gene plays a core regulatory role in V. mimicus virulence and pathogenicity.

show abstract

Section: Discussionmentioning

confidence: 99%