The Canadian medicinal plant Heracleum maximum contains antimycobacterial diynes and furanocoumarins

Bioactivity-guided isolation of antimicrobial coumarins from Heracleum mantegazzianum Sommier & Levier (Apiaceae) fruits by high-performance counter-current chromatography

“…Ojala et al (2000) showed the moderate activity of xanthotoxin against strains of B. subtilis and S. aureus, whereas our research showed very strong activity against these bacterial strains (MIC = 0.03 mg mL À1 ). Similarly, our study, bergapten showed strong inhibition of C. albicans ATCC 2091 (MIC = 0.125 mg mL À1 ) and M. luteus (MIC = 0.125 mg mL À1 ), although previously it had not shown activity (Ojala et al, 2000), which itself is in conflict with previous reports on the antibacterial activity of bergapten, as well as (Kousha & Bayat, 2012;O'Neill et al, 2013;Rosselli et al, 2003).…”

“…2e) and consequently a more detailed study was performed. Previously, pimpinellin was evaluated only against mycobacterium (O'Neill et al, 2013). In this study we found that pimpinellin exhibit moderate activity against Gram-positive bacteria, in particular S. aureus and M. luteus (MIC = 0.25 mg mL À1 ) and stronger activity against C. parapsilosis (MIC = 0.125 mg mL À1 ).…”

“…Compounds were identified using the mass spectra of reference compounds, as well as MS data from the literature (Li et al, 2014;Liu et al, 2011;O'Neill et al, 2013).…”

“…At the same time it is known that antimicrobial substances from natural sources have been investigated to achieve higher levels of food safety (Sakunpak & Panichayupakaranant, 2012). Extracts of the different Heracleum genus are widely used in the treatment of bacterial diseases in traditional medicine (Kousha & Bayat, 2012;O'Neill, Johnson, Webster, & Gray, 2013). The methanolic extract of Heracleum maximum W. Bartram roots, and the furanocoumarins bergapten, isobergapten, angelicin, sphondin, pimpinellin, isopimpinellin and 6-isopentenyloxyisobergapten, showed strong antimycobacterial activity (O'Neill et al, 2013).…”

“…Extracts of the different Heracleum genus are widely used in the treatment of bacterial diseases in traditional medicine (Kousha & Bayat, 2012;O'Neill, Johnson, Webster, & Gray, 2013). The methanolic extract of Heracleum maximum W. Bartram roots, and the furanocoumarins bergapten, isobergapten, angelicin, sphondin, pimpinellin, isopimpinellin and 6-isopentenyloxyisobergapten, showed strong antimycobacterial activity (O'Neill et al, 2013). In addition, Kousha and Bayat (2012) observed that the ethanolic extract of flowers and leaves of Heracleum persicum Desf.…”