1997
DOI: 10.1128/jb.179.23.7210-7218.1997
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The Candida albicans CDR3 gene codes for an opaque-phase ABC transporter

Abstract: We report the cloning and functional analysis of a third member of the CDR gene family in Candida albicans, named CDR3. This gene codes for an ABC (ATP-binding cassette) transporter of 1,501 amino acids highly homologous to Cdr1p and Cdr2p (56 and 55% amino acid sequence identity, respectively), two transporters involved in fluconazole resistance in C. albicans. The predicted structure of Cdr3p is typical of the PDR/CDR family, with two similar halves, each comprising an N-terminal hydrophilic domain with cons… Show more

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Cited by 97 publications
(70 citation statements)
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“…Interestingly, in contrast to Cdr3p, two other members of the C. albicans Cdr family, Cdr1p and Cdr2p, have been characterized as outward phospholipid transporters [63]. Cdr3p possess 56 and 55% sequence identity to Cdr1p and Cdr2p, respectively, and based on secondary structure predictions, would be expected to fold similarly to these and other Cdr proteins [66]. Likewise, human ABCA4 and its closest homologue, ABCA1, have a similar predicted architecture, but transport phospholipids in opposite directions [65].…”
Section: General Structural Features Of Abc Transportersmentioning
confidence: 99%
“…Interestingly, in contrast to Cdr3p, two other members of the C. albicans Cdr family, Cdr1p and Cdr2p, have been characterized as outward phospholipid transporters [63]. Cdr3p possess 56 and 55% sequence identity to Cdr1p and Cdr2p, respectively, and based on secondary structure predictions, would be expected to fold similarly to these and other Cdr proteins [66]. Likewise, human ABCA4 and its closest homologue, ABCA1, have a similar predicted architecture, but transport phospholipids in opposite directions [65].…”
Section: General Structural Features Of Abc Transportersmentioning
confidence: 99%
“…CDR3 expression, on the other hand, is linked to phenotypic switching (white-opaque transition) of C. albicans, while no apparent function has been ascribed to CDR4 (Balan et al, 1997;Sanglard et al, 1997;Franz et al, 1998). In this work we have used NBDtagged phospholipid analogues to explore whether Cdrps other than Cdr1p could also mediate transbilayer movement of membrane phospholipids.…”
Section: Introductionmentioning
confidence: 99%
“…The resistance to azoles in C. albicans, was earlier thought to occur primarily through an alteration or an overexpression of the 14a-lanosterol demethylase (P45014DM) involved in sterol biosynthesis (Lamb et al, 1997;White, 1997). However, the characterization of the first ATP-binding cassette (ABC) protein Cdr1p (Candida drug resistance) by us Krishnamurthy et al, 1998a, b, c;Prasad et al, 1995a;Smriti et al, 1999) and the subsequent identification of other efflux pumps (Balan et al, 1997;Sanglard et al, 1997Sanglard et al, , 1999Calabrese et al, 2000;Franz et al, 1998) and their overexpression in azole-resistant clinical isolates has led to the suggestion that these transporters represent another mechanism involved in multidrug resistance (MDR) scenario of C. albicans (Balan et al, 1997;Sanglard et al, 1997Sanglard et al, , 1999Gupta et al, 1998;Krishnamurthy et al, 1998;Smriti et al, 1999;Calabrese et al, 2000;Franz et al, 1998). In spite of the widespread occurrence of the MDR genes in C. albicans, the molecular mechanisms of their function and the regulation of their expression is yet to be understood (Prasad et al, 1995a.…”
Section: Introductionmentioning
confidence: 99%
“…Four kinds of CDR genes in C albicans have been cloned [13,[40][41][42] , but only over-expression of CDR1 and CDR2 resulted in azole resistance [43] . Tac1p is the major transcription factor needed for the regulation of CDR1 and CDR2, and is characterized by a highly conserved Zn(II) 2 Cys 6 zinc finger motif formed by six cysteines that coordinate two zinc atoms within the DNAbinding domain [11,44,45] .…”
Section: Discussionmentioning
confidence: 99%