The Capability of Neonatal Leukocytes to Produce IL-6 on Stimulation Assessed by Whole Blood Culture

Yachie, Akihiro; Takano, Nobuhiko; Yokoi, Takeshi; Kato, Koroku; Kasahara, Yoshihito; Miyawaki, Toshio; Taniguchi, Noboru

doi:10.1203/00006450-199003000-00005

Cited by 57 publications

(23 citation statements)

References 10 publications

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“…Our data also confirm previous studies showing similar production of TNF· , IFNÁ [8] and IL6 [7,9] in stimulated cord as in adult blood.…”

Section: Discussionsupporting

confidence: 82%

“…While the exact reason for this is not fully understood, immature complement receptors on neutrophils [3], deficient neutrophil adhesion and migration [4] and decreased capability of monocytes to produce cytokines which are involved in the acutephase response [5,6] are discussed to be among responsible factors. Decreased cytokine production by neonatal mononuclear cells, however, remains controversial, depending mostly on whether isolated mononuclear cells or whole blood are stimulated [7][8][9]. As yet, the capability of cord blood as a whole (reflecting physiological conditions in which intercellular interactions are respected) to produce both pro-and anti-inflammatory cytokines has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Production of Pro- and Anti- Inflammatory Cytokines in Neonates Assessed by Stimulated Whole Cord Blood Culture and by Plasma Levels at Birth

Seghaye¹,

Heyl

Grabitz³

et al. 1998

Neonatology

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The capability of neonates to achieve cytokine balance was evaluated. Production of the pro-inflammatory cytokines TNFα and IL-8, of the natural anti-inflammatory cytokine IL10 and of the regulator of the acute phase response IL6 was assessed after whole blood stimulation by lipopolysaccharide in cord blood (n = 10), adult volunteers serving as control (n = 17). Additionally, circulating cytokines were determined in cord and in maternal blood immediately after delivery (n = 27, respectively). Significant production of TNFα, IL8, IL10 and IL6 was observed in cord blood after lipopolysaccharide stimulation and was similar to cytokine production in adult blood. The plasma concentrations of TNFα were significantly higher in cord than in maternal blood, while plasma concentrations of IL10 and IL6 were significantly lower. Our results demonstrate fully developed capability of whole cord blood to synthesize pro- and anti-inflammatory cytokines in response to a pro-inflammatory stimulation in vitro. In vivo, however, higher circulating TNFα and lower IL10 and IL6 levels in cord blood suggest that the inflammatory stress associated with normal delivery does not induce detectable anti-inflammatory response in neonates at birth.

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“…Our data also confirm previous studies showing similar production of TNF· , IFNÁ [8] and IL6 [7,9] in stimulated cord as in adult blood.…”

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

The Production of Pro- and Anti- Inflammatory Cytokines in Neonates Assessed by Stimulated Whole Cord Blood Culture and by Plasma Levels at Birth

Seghaye¹,

Heyl

Grabitz³

et al. 1998

Neonatology

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“…IL-6 is produced by several cell types and is a potent stimulus to SAA and CRP production in cell lines and in studies of promoters for these acute phase genes linked to reporter genes (34,35). Production of IL-6 has been documented in fetal and neonatal mononuclear cells (36,37) and, although differences in action of IL-6 upon fetal hematopoietic progenitors compared with adult blood precursors have been described (38), the mechanism of such developmental differences remains to be elucidated. Data in this report suggest an immaturity of the mechanism involved in stimulation of CRP and SAA biosynthesis by IL-6 as well as IL-1 and TNF in young hamsters.…”

Section: Discussionmentioning

confidence: 99%

Developmental Regulation of Expression of C-Reactive Protein and Serum Amyloid A in Syrian Hamsters

1991

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ABSTRACT. The fetal and maternal concentration of various plasma proteins alters during pregnancy. Cells in the livers of fetal hamsters accumulate serum amyloid A (SAA) and C-reactive protein (CRP) mRNA, major acute phase reactants, when lipopolysaccharide is administered to the fetal circulation. No fetal SAA or CRP mRNA response is seen when the mother is stimulated at a remote site by endotoxin or a nonspecific inflammatory agent. In addition, cells of the fetal hamster liver do not respond by accumulating SAA mRNA when exposed to the specific cytokines, tumor necrosis factor, IL-1, and IL-6. CRP mRNA levels increased in fetal livers after administration of tumor necrosis factor and IL-1. These data suggest that cells contained in the fetal liver can respond during an acute phase reaction but that the capacity of some acute phase reactant genes to respond to cytokines may be developmentally regulated. Studies of immature hamsters after birth show that the responses of CRP and SAA genes to lipopolysaccharide, tumor necrosis factor, IL-1, and IL-6 are reduced when compared with induction of mRNA accumulation for these acute phase reactants in adult animals. (Pediatr Res 30: [444][445][446][447][448][449]1991)

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“…Reports concerning the generation of various cytokines by neonatal cells are controversial. While some investigators have shown comparable ability to produce IL-1 and IL-6 by preterm or term MC [32][33][34][35], others have found a reduced ability to secrete IL-1, IL-6, and TNF-· in comparison with adults [33,[36][37][38]. The hereby observed variations between MC of preterm newborns, term neonates, and adults in the spontaneous secretion of the cytokines, compared with that induced by LPS, suggest a difference in cell maturation stage and/or in the ability of the cells to respond to an exogenous stimulus.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dexamethasone on IL-1β, IL-6, and TNF-α Production by Mononuclear Cells of Newborns and Adults

Bessler

Mendel

Straussberg³

et al. 1999

Neonatology

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The effects of dexamethasone on the production of interleukin (IL) 1β, IL-6, and tumor necrosis factor alpha were studied in preterm newborns, term infants, and adults. Twenty preterm and 22 term newborns and 30 healthy adults were included in the study. Mononuclear cells (MC) isolated from cord blood of newborns and peripheral blood of adults were incubated without or with lipopolysaccharide in the absence or presence of dexamethasone at concentrations between 10^–8 and 10^–5 M. The cytokine concentration in the supernatants was tested using enzyme-linked immunosorbent assay kits. Although a dose-dependent inhibition of the cytokine production was observed at pharmacological doses of dexamethasone in individuals of the three groups, differences in the intensity of the effect were observed between the groups. Spontaneous secretion of IL-1β or IL-6 by MC of preterm neonates was less inhibited by dexamethasone as compared with cells from adults. In contrast, the inhibitory effect of the drug on lipopolysaccharide-induced IL-6 and tumor necrosis factor alpha production was more pronounced on neonatal cells. As for term newborns, MC were more sensitive to the inhibitory effect of the drug on LPS-induced IL-6 production than cells of adults. The results suggest that dexamethasone treatment of preterm newborns may affect cytokine production with a consequent modulation of the host’s immune response.

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The Capability of Neonatal Leukocytes to Produce IL-6 on Stimulation Assessed by Whole Blood Culture

Cited by 57 publications

References 10 publications

The Production of Pro- and Anti- Inflammatory Cytokines in Neonates Assessed by Stimulated Whole Cord Blood Culture and by Plasma Levels at Birth

The Production of Pro- and Anti- Inflammatory Cytokines in Neonates Assessed by Stimulated Whole Cord Blood Culture and by Plasma Levels at Birth

Developmental Regulation of Expression of C-Reactive Protein and Serum Amyloid A in Syrian Hamsters

Effects of Dexamethasone on IL-1β, IL-6, and TNF-α Production by Mononuclear Cells of Newborns and Adults

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