The capacity of hydrogenotrophic anaerobic bacteria to compete for traces of hydrogen depends on the redox potential of the terminal electron acceptor

Cord‐Ruwisch, Ralf; Seitz, Hans-Jürgen; Conrad, Ralf

doi:10.1007/bf00411655

Cited by 489 publications

(303 citation statements)

References 43 publications

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“…Methane production started as soon as sulfate was depleted. This observation is in accordance with the well-known facts about substrate affinity and competition between sulfate reducers and methanogens (Oremland and Taylor, 1977;Cord-Ruwisch et al, 1988).…”

Section: Fermentation Productssupporting

confidence: 92%

Degradation of cyanobacterial biomass in anoxic tidal-flat sediments: a microcosm study of metabolic processes and community changes

2011

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To follow the anaerobic degradation of organic matter in tidal-flat sediments, a stimulation experiment with 13 C-labeled Spirulina biomass (130 mg per 21 g sediment slurry) was conducted over a period of 24 days. A combination of microcalorimetry to record process kinetics, chemical analyses of fermentation products and RNA-based stable-isotope probing (SIP) to follow community changes was applied. Different degradation phases could be identified by microcalorimetry: Within 2 days, heat output reached its maximum (55 lW), while primary fermentation products were formed (in lmol) as follows: acetate 440, ethanol 195, butyrate 128, propionate 112, H 2 127 and smaller amounts of valerate, propanol and butanol. Sulfate was depleted within 7 days. Thereafter, methanogenesis was observed and secondary fermentation proceeded. H 2 and alcohols disappeared completely, whereas fatty acids decreased in concentration. Three main degraders were identified by RNA-based SIP and denaturant gradient gel electrophoresis. After 12 h, two phylotypes clearly enriched in 13 C: (i) Psychrilyobacter atlanticus, a fermenter known to produce hydrogen and acetate and (ii) bacteria distantly related to Propionigenium. A Cytophaga-related bacterium was highly abundant after day 3. Sulfate reduction appeared to be performed by incompletely oxidizing species, as only sulfate-reducing bacteria related to Desulfovibrio were labeled as long as sulfate was available.

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Section: Fermentation Productssupporting

confidence: 92%

Degradation of cyanobacterial biomass in anoxic tidal-flat sediments: a microcosm study of metabolic processes and community changes

2011

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“…Indications are often the production of H 2 S and the resulting corrosion problems [22]. Other sources [8] and [23] give hints that also acetogenic archaea and iron-reducing bacteria could be stimulated and contribute in the metabolism of hydrogen. According to the available literature, it is expected that four hydrogenotrophic microbial processes could be important for UHS: -Methanogenesis:…”

Section: Bio-chemical Reactions In Uhsmentioning

confidence: 99%

“…The coexistence of several species can thereby result in a concurrence between the different hydrogenotrophic processes which was investigated by [8] and [23]. They have shown that a simultaneous survival and also an out-competition is possible.…”

Section: Bio-chemical Reactions In Uhsmentioning

confidence: 99%

Hydrogenization of underground storage of natural gas

et al. 2015

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The intermittent production of the renewable energy imposes the necessity to temporarily store it. Large amounts of exceeding electricity can be stored in geological strata in the form of hydrogen. The conversion of hydrogen to electricity and vice versa can be performed in electrolyzers and fuel elements by chemical methods. The nowadays technical solution accepted by the European industry consists of injecting small concentrations of hydrogen in the existing storages of natural gas. The progressive development of this technology will finally lead to the creation of underground storages of pure hydrogen. Due to the low viscosity and low density of hydrogen, it is expected that the problem of an unstable displacement, including viscous fingering and gravity overriding, will be more pronounced. Additionally, the injection of hydrogen in geological strata could encounter chemical reactivity induced by various species of microorganisms that consume hydrogen for their metabolism. One of the products of such reactions is methane, produced from Sabatier reaction between H 2 and CO 2 . Other hydrogenotrophic reactions could be caused by acetogenic archaea, sulfate-reducing bacteria and iron-reducing bacteria. In the present paper, a mathematical model is presented which is capable to reflect DuMuX was used to model the evolution of a hypothetical underground storage of hydrogen. We have revealed that the behavior of an underground hydrogen storage is different than that of a natural gas storage. Both, the hydrodynamic and the bio-chemical effects, contribute to the different characteristics.

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“…The ambient H 2 concentration is dynamically controlled and is generally indicative of the dominant terminal electron-accepting process. In general, organisms that respire NO 3 -, Fe(III), or Mn(IV) are able to outcompete sulfate-reducing bacteria for H 2 , which in turn outcompete methanogens (Conrad and Wetter 1990;Cord-Ruwisch et al 1988;Hoehler et al 1998;Lovley and Phillips 1987;Lovley and Goodwin 1988). Hydrogen concentration generally varies by orders of magnitude between such different redox environments, with the highest H 2 levels present in methanogenic environments.…”

Section: Introductionmentioning

confidence: 99%

Hydrogen production by methanogens under low-hydrogen conditions

Valentine

Blanton

Reeburgh

2000

Archives of Microbiology

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Hydrogen production was studied in four species of methanogens (Methanothermobacter marburgensis, Methanosaeta thermophila, Methanosarcina barkeri, and Methanosaeta concilii) under conditions of low (sub-nanomolar) ambient hydrogen concentration using a specially designed culture apparatus. Transient hydrogen production was observed and quantified for each species studied. Methane was excluded as the electron source, as was all organic material added during growth of the cultures (acetate, yeast extract, peptone). Hydrogen production showed a strong temperature dependence, and production ceased at temperatures below the growth range of the organisms. Addition of polysulfides to the cultures greatly decreased hydrogen production. The addition of bromoethanesulfonic acid had little influence on hydrogen production. These experiments demonstrate that some methanogens produce excess reducing equivalents during growth and convert them to hydrogen when the ambient hydrogen concentration becomes low. The lack of sustained hydrogen production by the cultures in the presence of methane provides evidence against "reverse methanogenesis" as the mechanism for anaerobic methane oxidation.

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The capacity of hydrogenotrophic anaerobic bacteria to compete for traces of hydrogen depends on the redox potential of the terminal electron acceptor

Cited by 489 publications

References 43 publications

Degradation of cyanobacterial biomass in anoxic tidal-flat sediments: a microcosm study of metabolic processes and community changes

Degradation of cyanobacterial biomass in anoxic tidal-flat sediments: a microcosm study of metabolic processes and community changes

Hydrogenization of underground storage of natural gas

Hydrogen production by methanogens under low-hydrogen conditions

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