The capacity of phototrophic sulfur bacterium Thiocapsa roseopersicina for chemosynthesis

Kondratieva, E. N.; Жуков, В. Г.; Ivanovsky, R. N.; Petushkova, Yu. P.; Monosov, Edward

doi:10.1007/bf00454854

Cited by 69 publications

(31 citation statements)

References 17 publications

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“…The in vivo absorption spectra of both strains indicate the presence of bacteriochlorophyll a (Fig. 2) (Kondratieva et al, 1976;Puchkova et al, 2000;Herbert et al, 2005). Both strains are catalase-positive, utilize many organic compounds phototrophically, and also grow aerobically in the dark with malate (strain LQ17) or fructose (strain KS1) as electron and carbon source.…”

Section: Discussionmentioning

confidence: 91%

Anaerobic phototrophic nitrite oxidation by Thiocapsa sp. strain KS1 and Rhodopseudomonas sp. strain LQ17

2010

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In anaerobic enrichment cultures for phototrophic nitrite-oxidizing bacteria from different freshwater sites, two different cell types, i.e. non-motile cocci and motile, rod-shaped bacteria, always outnumbered all other bacteria. Most-probable-number (MPN) dilution series with samples from two freshwater sites yielded only low numbers (¡3¾10 3 cm "3 ) of phototrophic nitrite oxidizers. Slightly higher numbers (about 10 4 cm "3 ) were found in activated sewage sludge. Anaerobic phototrophic oxidation of nitrite was studied with two different isolates, the phototrophic sulfur bacterium strain KS1 and the purple nonsulfur bacterium strain LQ17, both of which were isolated from activated sludge collected from the municipal sewage treatment plant in Konstanz, Germany. Strain KS1 converted 1 mM nitrite stoichiometrically to nitrate with concomitant formation of cell matter within 2-3 days, whereas strain LQ17 oxidized only up to 60 % of the given nitrite to nitrate within several months with the concomitant formation of cell biomass. Nitrite oxidation to nitrate was strictly light-dependent and required the presence of molybdenum in the medium. Nitrite was oxidized in both the presence and absence of oxygen. Nitrite inhibited growth at concentrations higher than 2 mM. Hydroxylamine and hydrazine were found to be toxic to the phototrophs in the range 5-50 mM and did not stimulate phototrophic growth. Based on morphology, substrate-utilization pattern, in vivo absorption spectra, and 16S rRNA gene sequence similarity, strain KS1 was assigned to the genus Thiocapsa and strain LQ17 to the genus Rhodopseudomonas. Also, Thiocapsa roseopersicina strains DSM 217 and DSM 221 were found to oxidize nitrite to nitrate with concomitant growth. We conclude that the ability to use nitrite phototrophically as electron donor is widespread in nature, but low MPN counts indicate that its contribution to nitrite oxidation in the studied habitats is rather limited.

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Section: Discussionmentioning

confidence: 91%

Anaerobic phototrophic nitrite oxidation by Thiocapsa sp. strain KS1 and Rhodopseudomonas sp. strain LQ17

2010

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“…For example, certain Chromatiaceae, including species of Allochromatium, Thiocystis, Amoebobacter, and Thiocapsa, can grow in darkness as either chemoorganotrophs or chemolithotrophs when the oxygen concentration is signifi cantly reduced [microaerobic growth; Kämpf and Pfennig, 1980]. Thiocapsa roseopersicina and Thiocystis violacea are the most oxygen tolerant purple sulfur bacteria (Kondratieva et al, 1976;Kämpf and Pfennig, 1980); however, respiratory growth of these species is very slow compared with phototrophic growth. If one considers that dark growth of purple sulfur bacteria in nature puts them in direct competition with nonphototrophic bacteria as well as with purple nonsulfur bacteria, the ecological signifi cance of dark metabolism by purple sulfur bacteria is probably minor.…”

Section: A Purple Sulfur Bacteriamentioning

confidence: 99%

An Overview of Purple Bacteria: Systematics, Physiology, and Habitats

Madigan

Jung

2009

Advances in Photosynthesis and Respiration

126

117

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“…Batch culture experiments of Pfennig (1971), Bogorov (1974), Gorlenko (1974), Kondratieva et al (1976) and K/impf and Pfennig (1980Pfennig ( , 1986 revealed the capacity of Thiocapsa roseopersicina, Amoebobacter roseus, Thioc~,stis violacea, Chromatium vinosum, C. minus, C. violascens and C. gracile to grow in the dark by oxidizing reduced inorganic sulfur compounds with oxygen. In contrast, green sulfur bacteria did not grow chemotrophically (K/impf and Pfennig 1980).…”

mentioning

confidence: 99%

“…Under these conditions chemotrophically growing cells still contained photosynthetic pigments (Gorlenko 1974, Kondratieva et al 1976, K/impf and Pfennig 1986. Furthermore, growth of some strains under dark conditions continued for a limited period of time but ceased after repeated transfer into fresh culture medium (K/impf and Pfennig 1986).…”

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Continuous chemotrophic growth and respiration of Chromatiaceae species at low oxygen concentrations

Overmann

Pfennig

1992

Arch. Microbiol.

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Abstract.Endogenous and maximum respiration rates of nine purple sulfur bacterial strains were determined. Endogenous rates were below 10 nmol 0 2 9 (mg protein.min) -1 for sulfur-free cells and 15-35nmol 02 9 (mg protein 9 min)-1 for cells containing intracellular sulfur globules. With sulfide as electron-donating substrate respiration rates were considerably higher than with thiosulfate. Maximum respiration rates of Thiocystis violacea 2711 and Thiorhodovibrio winogradskyi SSP1 (254.8 and 264.2 nmol 02 " (mg protein-min)-1, respectively) are similar to those of aerobic bacteria. Biphasic respiration curves were obtained for sulfur-free cells of Thiocvstis violacea 2711 and Chromatium vinosum 2811. In Thiocystis violacea the rapid and incomplete oxidation of thiosulfate was five times faster than the oxidation of stored sulfur. A high affinity of the respiratory system for oxygen (Kin = 0.3 0.9 gM O2, Vm,x = 260nmo1 O2" (rag protein-min) -1 with sulfide as substrate, Kin=0.6 2.4gM 02, Vmax= 14-40nmo1 O2"(mg protein -min)-1 with thiosulfate as substrate), for sulfide (K~ = 0.47 ~tM, Vm,x = 650 nmol HzS 9 (rag protein x rain) -~, and for thiosulfate (K~ = 5-6 ~tM, Vm,x = 24-72nmol S 2 0 2 -. ( m g protein-rain) -1 was obtained for different strains. Respiration of Thiocvstis violacea was inhibited by very low concentrations of NaCN (Kz = 1.7 ~tM) while CO concentrations of up to 300 gM were not inhibitory. The capacity for chemotrophic growth of six species was studied in continuous culture at oxygen concentrations of 11 to 67 gM. Thiocystis violacea 2711, Amoebobacter roseus 6611, Thiocapsa roseopersicina 6311 and Thiorhodovibrio winogradskyi SSP1 were able to grow chemotrophically with thiosulfate/acetate or sulfide/acetate. Chromatium vinosum 2811 and Amoebobacter purpureus ML1 failed to grow under these conditions. During shift from phototrophic to chemotrophic conditions intracellular sulfur and carbohydrate accumulated transiently inside the * Present address and address for correspondence: J. Overmann, Department of Microbiology, University of British Columbia, 300-6174 Umversity Boulevard, Vancouver. B.C., Canada V6T 1Z3 cells. During chemotrophic growth bacteriochlorophyll a was below the detection limit.

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The capacity of phototrophic sulfur bacterium Thiocapsa roseopersicina for chemosynthesis

Cited by 69 publications

References 17 publications

Anaerobic phototrophic nitrite oxidation by Thiocapsa sp. strain KS1 and Rhodopseudomonas sp. strain LQ17

Anaerobic phototrophic nitrite oxidation by Thiocapsa sp. strain KS1 and Rhodopseudomonas sp. strain LQ17

An Overview of Purple Bacteria: Systematics, Physiology, and Habitats

Continuous chemotrophic growth and respiration of Chromatiaceae species at low oxygen concentrations

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