The Carbohydrate Nutrition and Metabolism of a Strain of Mammalian Cells (MB III Strain of Mouse Lymphoblasts) Growing in Vitro

Bailey, J. Martyn; Gey, George O.; Gey, Margaret K.

doi:10.1016/s0021-9258(18)98126-3

Cited by 27 publications

(1 citation statement)

References 10 publications

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“…To specifically label intracellular S Tm bacteria post-invasion of host cells, we searched for a carbon source that would (i) enter host cells, but not be utilized by them; (ii) be accessible and used by intracellularly growing bacteria; and (iii) minimally impair the native host and bacterial metabolism. S Tm can grow on a more diverse array of carbon sources [ 29 ] than mammalian cells [ 30 , 31 ]. Guided by the study by Steeb and colleagues [ 7 ] on nutrient availabilities for S Tm in a mouse infection model, we tested whether mannitol meets the above 3 requirements ( Fig 1A ).…”

Section: Resultsmentioning

confidence: 99%

A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella

Mitosch,

Beyß,

Phapale

et al. 2023

PLoS Biol

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Pathogenic bacteria proliferating inside mammalian host cells need to rapidly adapt to the intracellular environment. How they achieve this and scavenge essential nutrients from the host has been an open question due to the difficulties in distinguishing between bacterial and host metabolites in situ. Here, we capitalized on the inability of mammalian cells to metabolize mannitol to develop a stable isotopic labeling approach to track Salmonella enterica metabolites during intracellular proliferation in host macrophage and epithelial cells. By measuring label incorporation into Salmonella metabolites with liquid chromatography–mass spectrometry (LC–MS), and combining it with metabolic modeling, we identify relevant carbon sources used by Salmonella, uncover routes of their metabolization, and quantify relative reaction rates in central carbon metabolism. Our results underline the importance of the Entner–Doudoroff pathway (EDP) and the phosphoenolpyruvate carboxylase for intracellularly proliferating Salmonella. More broadly, our metabolic labeling strategy opens novel avenues for understanding the metabolism of pathogens inside host cells.

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Section: Resultsmentioning

confidence: 99%

A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella

Mitosch,

Beyß,

Phapale

et al. 2023

PLoS Biol

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Recovery of cells in vitro from the effects of hypoxia and hyperoxia

Shaw

Pace

1969

Journal Cellular Physiology

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The cytotoxic effect of high, as well as low, oxygen tension of proliferation and metabolism of Low line cells in culture is reversible even after several days of exposure provided the cells are returned to 95% air + 5% COZ environment.This suggests that the activity of certain mechanisms within the cells may have been altered or in other ways inhibited by the abnormal environments but are quite rapidly regenerated once the adverse condition is removed. The cells tolerate a low 0 2 exposure for at least 20 days while continuous exposure to high 0 2 atmosphere results in degeneration and death after 7-10 days. Both glucose utilization and lactic acid production are elevated in cultures exposed to either low or high 0 2 tensions, although they are markedly higher in the latter condition. When cell so exposed are returned to a n air + 5% COZ atmosphere, rate of glucose uptake and lactic acid formation soon approaches that found in control cultures. of the basic mechanisms of cultured cells to recover from the effects of cytotoxic environments. Therefore, the following investigations on the changes in growth and metabolism of cells exposed to low and high OZ tensions and the possible reversibility of these changes were undertaken. MATERIALS AND METHODSLow line fibroblast cells, NCTC 2049 (Sanford, Likely and Earle, '54), cultivated in NCTC 109 medium (Evans, Bryant, McQuilkin, Fioramonti, Sanford, Westfall and Earle, '56) plus 10% horse serum in pyrex T-flasks were used in these studies.Replicate techniques and gassing procedures were the same as reported earlier (Pace and Aftonomos, '57; Pace, Thompson, Aftonomos and Holck, '61). For replication in each of the following experiments, the cells were detached from the bottoms of the stock flasks by scraping and gentle shaking. After proper dilution, 2 ml of the resutling suspension were added to each of 150 T-15 flasks. Twenty-four hours were allowed for adjustment at which time counts were made (by means of a Coulter counter, Model B) and glucose and lactic acid (L.A.) content of the medium ascertained by means of the 0-toluidine (Hy-

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Pentose utilizing variants of Novikoff hepatoma cells: Modification of growth and morphological properties

Hoffee

Jargiello

Zaner

et al. 1977

Journal Cellular Physiology

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A series of variant lines that utilize multiple pentoses for growth in place of glucose have been isolated from an 8-azaguanine resistant line of Novikoff hepatoma cells (N1S167). These variants utilize for growth ribose, xylose, arabinose, and/or deoxyribose. The variants growing on pentose containing medium (a) exhibit a density dependent cessation of growth, (b) have a morphology change to a more flattened cell type, (c) become binucleated in the presence of cyto chalasin B, and (d) show an altered sensitivity to trypsin treatment.

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The Carbohydrate Nutrition and Metabolism of a Strain of Mammalian Cells (MB III Strain of Mouse Lymphoblasts) Growing in Vitro

Cited by 27 publications

References 10 publications

A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella

A pathogen-specific isotope tracing approach reveals metabolic activities and fluxes of intracellular Salmonella

Recovery of cells in vitro from the effects of hypoxia and hyperoxia

Pentose utilizing variants of Novikoff hepatoma cells: Modification of growth and morphological properties

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