The Caries Inhibitory Effects of GOS‐Sugar <i>in vitro</i> and in Rat Experiments

Ooshima, Takashi; Fujiwara, Taku; Takei, T; Izumitani, A; Sobue, S; Hamada, Shigeyuki

doi:10.1111/j.1348-0421.1988.tb01474.x

Cited by 36 publications

(23 citation statements)

References 18 publications

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“…Fructose, glucose-oligomers such as maltose, iso maltose and panose, and structural isomers of sucrose such as palatinose and trehalulose have been shown to inhibit insoluble glucan synthesis by GTases from sucrose and also to be cariostatic or noncariogenic in SPF rats infected with mutans streptococci [Ooshima et al, 1983a[Ooshima et al, , b. 1986[Ooshima et al, ,1988[Ooshima et al, . 1991a.…”

Section: Discussionmentioning

confidence: 99%

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Oolong Tea Polyphenols Inhibit Experimental Dental Caries in SPF Rats Infected with Mutatis Streptococci

Ooshima¹,

Minami²,

W³

et al. 1993

Caries Res

112

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An extract of oolong tea (semifermented tea leaves of Camellia sinensis) and its chromatographically isolated polyphenolic compound was examined for in vitro inhibitory effects on glucosyltransferases (GTases) of mutans streptococci and on caries development in Sprague-Dawley rats infected with mutans streptococci. The samples showed no detectable effect on the growth of mutans streptococci. However, insoluble glucan synthesis from sucrose by the GTases of Streptococcus mutans MT8148R and Streptococcus sobrinus 6715 was markedly inhibited, as was sucrose-dependent cell adherence of these mutans streptococci. The administration of the oolong tea extract and the isolated polyphenol compound into diet 2000 and drinking water resulted in significant reductions in caries development and plaque accumulation in the rats infected with mutans streptococci. The active components in the oolong tea extract were presumptively identified as polymeric polyphenols which were specific for oolong tea leaves. These results indicate that the oolong tea polyphenolic compounds could be useful for controlling dental caries.

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Section: Discussionmentioning

confidence: 99%

“…The jaws were immersed in 4.5 ml of sterile saline and ultrasonicated for 20 s at 20 W at the maximum amplitude using a sonic oscillator (model DR-20P, Tomy Seiko Co., Tokyo, Japan) to disperse the dental plaque on the tooth surface. The suspensions were decimal diluted with sterile saline and streaked on agar plates as described above [Ooshima et al, 1988].…”

mentioning

confidence: 99%

Oolong Tea Polyphenols Inhibit Experimental Dental Caries in SPF Rats Infected with Mutatis Streptococci

Ooshima¹,

Minami²,

W³

et al. 1993

Caries Res

112

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show abstract

“…The dose was doubled on day 22 following the growth of rats. An oral swab was taken from individual rats at weekly intervals, which permitted semiquantitative microbiological examination (20). At the end of the experiment (73 days of age), the rats were sacrificed and the mandibles were removed aseptically.…”

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confidence: 99%

Passive Immunization with Bovine Milk Containing Antibodies to a Cell Surface Protein Antigen-Glucosyltransferase Fusion Protein Protects Rats against Dental Caries

et al. 2002

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Cell surface protein antigen (PAc) and glucosyltransferases (GTF) of Streptococcus mutans are major colonization factors of the organism. We prepared bovine milk containing antibodies against a fusion of the saliva-binding alanine-rich region of PAc with the glucan-binding domain of GTF-I. This study examined the effect of the immune milk on the cariogenicity of S. mutans in a rat model. Concentrated immune milk was fed to rats once a day for 55 days. The group that received immune milk had significantly less caries development than controls.

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“…These various enzymes in the GH13 family can be utilized in many different industrial applications. For example panose (from neopullulanase degradation) is used as a sweetener, and maltogenic amylases can be used as an antistaling agent in bread and for production of maltose syrup (Ooshima et al 1988;van der Maarel et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Differences and similarities in enzymes from the neopullulanase subfamily isolated from thermophilic species

et al. 2008

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Six glycoside hydrolase (GH) family 13 members, classified under the polyspecific neopullulanase subfamily GH13 20 (also termed cyclomaltodextrinase) were analysed. They originate from thermophilic bacterial strains (Anoxybacillus flavithermus, Laceyella sacchari, and Geobacillus thermoleovorans) or from environmental DNA, collected after in situ enrichments in Icelandic hot springs. The genes were isolated following the CODEHOP consensus primer strategy, utilizing the first two of the four conserved sequence regions in GH13. The typical domain structure of GH13 20, including an N-terminal domain (classified as CBM34), the catalytic module composed of the A-and B-domains, and a C-terminal domain, was found in five of the encoded enzymes (abbreviated Amy1, 89, 92, 98 and 132). These five enzymes degraded cyclomaltodextrins (CDs) and starch, while only three, Amy92 (L. sacchari), Amy98 (A. flavithermus) and Amy132 (environmental DNA), also harboured neopullulanase activity. The L. sacchari enzyme was monomeric, but with CD as the preferred substrate, which is an unusual combination. The sixth enzyme (Amy29 from environmental DNA), was composed of the ABC-domains only. Preferred substrate for Amy29 was pullulan, which was degraded to panose, and the enzyme had no detectable activity on CDs. In addition to its different activity profile and domain composition, Amy29 also displayed a different conservation (LPKF) in the fifth conserved region (MPKL) proposed to identify the subfamily. All enzymes had apparent temperature optima in the range 50-65• C, while thermostability varied, and was highest for Amy29 with a half-life of 480 min at 80• C. Calcium dependent activity or stability was monitored in four enzymes, but could not be detected for Amy29 or 98. Tightly bound calcium can, however, not be ruled out, and putative calcium ligands were conserved in Amy98.

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The Caries Inhibitory Effects of GOS‐Sugar in vitro and in Rat Experiments

Cited by 36 publications

References 18 publications

Oolong Tea Polyphenols Inhibit Experimental Dental Caries in SPF Rats Infected with Mutatis Streptococci

Oolong Tea Polyphenols Inhibit Experimental Dental Caries in SPF Rats Infected with Mutatis Streptococci

Passive Immunization with Bovine Milk Containing Antibodies to a Cell Surface Protein Antigen-Glucosyltransferase Fusion Protein Protects Rats against Dental Caries

Differences and similarities in enzymes from the neopullulanase subfamily isolated from thermophilic species

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