SummaryThis paper summarises results of carotenoid and vitamin A alcohol assays on a large number of individual blood samples taken from groups of pasture-fed dairy cows throughout the 1<954-55 to 1958-59 dairying seasons. Marked seasonal variations are shown to occur each year and attention is particularly drawn to the very large variability in the blood levels of carotenoids and vitamin A alcohol in individual animals of the 3ame breed.The effect, under pasture-feeding conditions, of factors such as breed, age, sex, and lactation on blood carotenoid and vitamin A alcohol levels is also reported. It is shown that, under New Zealand conditions of high carotene intake throughout the year, the relationship between blood and milk-fat carotenoid levels is very different from that reported by overseas workers for lower carotene intakes.