The aim of this research work was to assess the effect of the microfiltration (ceramic membranes 1.4 µm, 50 • C) of partially defatted ovine milk (fat 0.4%) and bovine milk (fat 0.3%) characteristics. Feed milks, permeates and retentates were analyzed for microbial counts, gross composition, protein fractions, the indigenous enzymes cathepsin D and alkaline phosphatase and the behavior during renneting. It was showed that the microbial quality of both ovine and bovine permeate was improved by reduction of the total mesophilic microflora about 4 Log and 2 Log, respectively. The protein contents and the total solids contents of both permeates were significantly (p < 0.05) reduced. A further analysis of protein fractions by Reversed Phase -High Performance Liquid Chromatography (RP-HPLC) revealed lower αs 1 -and β-casein and higher κ-casein contents in permeates. The activity of alkaline phosphatase followed the allocation of the fat content, while activity of cathepsin D in permeates was not influenced, although somatic cells counts were removed. Regarding cheesemaking properties, the firmness of ovine curd made from the feed milk did not differ significantly from that made from the permeate. The obtained results suggested that microfiltration could be used for pre-treating of ovine milk prior to cheesemaking.Foods 2020, 9, 284 2 of 11 the two-months rolling geometric average of microbes (with at least two samples per month) grown on Plate Count Agar at 30 • C should be a maximum 1,500,000 per mL. [15]. To the best of our knowledge, studies for MF of ovine milk are rare and concern only the impact on microflora [16,17]. Moreover, there is lack of information about the influence of MF on indigenous enzymes or on the renneting behavior of either ovine or bovine milk. Therefore, the aim of this study is to evaluate the effect of MF, using a ceramic membrane of 1.4 µm pore size, on the composition, microflora, cathepsin D and alkaline phosphatase as well as on cheesemaking properties of ovine and bovine milk.
Materials and Methods
MicrofiltrationRaw ovine and bovine milk obtained from the farm of Agricultural University of Athens were defatted to fat content 0.30% and 0.40%, respectively, using a laboratory milk fat separator, and were subsequently heated at 50 • C. Crossflow MF processing (Figure 1) took place on a pilot microfiltration module (PALL Italia s.r.l, MILANO, Italy) under a constant transmembrane pressure (TMP) of 1.5 bar at 50 • C using ceramic membrane P19-40 (Membralox ® ) of 1.4 µm pore size, with length 1020 mm and area 0.24 m 2 . Filtration duration was 15 min with constant flux at 105 ± 32 L·m −2 ·h −1 for ovine milk and 314 ± 32 L·m −2 ·h −1 for bovine milk. Retention flow rates were 363.23 ± 86.32 L·m −2 ·h −1 and 795.83 ± 18.37 L·m −2 ·h −1 for bovine and ovine milk retentates, respectively. After processing, the microfiltration unit was cleaned by rinsing in series with water, NaOH 1% solution, water, HNO 3 1% solution and water to reach pH 7.0 [17]. Five and three experimental trials were carri...