The CatSper calcium channel in human sperm: relation with motility and involvement in progesterone-induced acrosome reaction

Tamburrino, Lara; Marchiani, Sara; Minetti, Federico; Forti, Gianni; Muratori, Monica; Baldi, Elisabetta

doi:10.1093/humrep/det454

Cited by 108 publications

(135 citation statements)

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“…Furthermore, a previous study revealed that lead could inhibit voltage-dependent calcium channel currents in nerve cells (Busselberg et al 1991;Gawrisch et al 1997). In human spermatozoa, the voltagedependent calcium channel, CatSper, is regarded as the main channel to mediate Ca 2+ influx and regulate progesterone-induced acrosome reaction (Strunker et al 2011;Tamburrino et al 2014). Hence, lead treatment probably decreased [Ca 2+ ] i and inhibited the progesterone-induced acrosome reaction via a CatSper-related mechanism.…”

Section: Discussionmentioning

confidence: 99%

Lead Inhibits Human Sperm Functions by Reducing the Levels of Intracellular Calcium, cAMP, and Tyrosine Phosphorylation

Zou

Chen

et al. 2016

Tohoku J. Exp. Med.

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It is well known that there has been a worldwide decrease in human male fertility in recent years. One of the main factors affecting this is environmental pollution. Lead is one of the major heavy metal contaminants that threaten the health of animals and human beings in China. It preferentially accumulates in male reproductive organs and can be up to 10 µM in human seminal plasma. Lead impairs mammalian spermatogenesis and sperm quality in vivo. It also inhibits sperm functions in vitro but the underlying mechanisms remain unclear. Therefore, we aimed to investigate the in vitro toxicity of lead on human sperm functions and to elucidate the underlying mechanisms. Semen samples were collected from 20 healthy volunteers with different careers and backgrounds living in Nanchang, Jiangxi. Human sperm suspensions were treated with different concentrations of lead acetate (0, 0.5, 2.5, 10, 50, and 100 µM) and the viability, motility, capacitation and progesterone-induced acrosome reaction were examined. Treatment with 10-100 µ M lead acetate dose-dependently inhibited total and progressive motility measures, capacitation and progesterone-induced acrosome reaction. It also dose-dependently decreased the intracellular concentrations of cyclic adenosine monophosphate (cAMP) and calcium ([Ca 2+ ] i ), and reduced the tyrosine phosphorylation of sperm proteins, all of which are thought to be key factors in the regulation of sperm function. Our findings suggest that lead inhibits human sperm functions by reducing the levels of sperm intracellular cAMP, [Ca 2+ ] i and tyrosine phosphorylation of sperm proteins in vitro.

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Section: Discussionmentioning

confidence: 99%

Lead Inhibits Human Sperm Functions by Reducing the Levels of Intracellular Calcium, cAMP, and Tyrosine Phosphorylation

Zou

Chen

et al. 2016

Tohoku J. Exp. Med.

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“…In human sperm, Ca 2þ influx is predominantly controlled by CatSper [28], which is closely associated with human sperm functions. It has been reported that CatSper expression correlates with human sperm motility [24]. Also, CatSper is regarded as the effector of progesterone and participates in the progesterone-induced acrosome reaction [24].…”

Section: Discussionmentioning

confidence: 99%

“…To further determine the underlying mechanism of ketamine caused sperm [Ca 2þ ] i decrease, followed by impaired human sperm functions, we examined the effect of ketamine on CatSper channel, because (1) CatSper is the sperm-specific and the predominant ion channel responsible for Ca 2þ influx, and it is associated with sperm motility [24]; (2) the sperm ability to penetrate through viscous medium is attributed to the activation of CatSper [14]; and (3) CatSper is involved in the progesterone-induced human sperm acrosome reaction [24]. To data, CatSper is a unique Ca 2þ channel that can use the patch-clamp technique for recording currents in human sperm [18].…”

Section: Ketamine Suppresses Human Sperm Catsper Currentsmentioning

confidence: 99%

Ketamine inhibits human sperm function by Ca2+-related mechanism

Zou

et al. 2016

Biochemical and Biophysical Research Communications

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“…Boar spermatozoa express the four subunits of the main sperm Ca 2+ channel (CatSper;Lishko et al 2011;Tamburrino et al 2014) in their plasma membrane. The CatSper becomes functional when boar spermatozoa are exposed to capacitation-inducing conditions; modulating sperm motility (Paper II).…”

Section: Discussionmentioning

confidence: 99%

“…Among the most important events regulated by sperm membrane proteins appear volume accomodation via AQPs [Tamburrino et al 2014] and opioid receptors [Agirregoitia et al 2006;) and the sperm-oocyte interaction and fusion via sperm membrane proteins (IZUMO1-JUNO, Aydin et al 2016;Ohto et al 2106). The main sperm membrane channels and receptors identified in pig, mouse and human spermatozoa are listed in Table 1.…”

Section: Sperm Membrane Proteinsmentioning

confidence: 99%

Sperm Membrane Channels, Receptors and Kinematics : Using boar spermatozoa for drug toxicity screening

Vicente-Carrillo¹

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About the cover:The figure in the front cover displays boar spermatozoa where AQP-9 (upper-left corner), CatSper 3 (upper-right corner) and the δ-opioid receptor (lower-left corner) are immunolocalized. A representative output of the Qualisperm™ software depicting each detected spermatozoon and its motility trajectory (in yellow) is provided in the lower-right corner.During the course of the research underlying this Thesis, Alejandro Vicente Carrillo was enrolled in Forum Scientium, a multidisciplinary doctoral program at Linköping University. Printed by LiU-Tryck, Linköping, Sweden, 2016 To my family MAIN SUPERVISOR Heriberto Rodríguez-Martínez ABSTRACTInternal fertilization usually implies that a spermatozoon, with intact attributes for zygote formation, passes all hurdles during its transport through the female genitalia and reaches the oocyte. During this journey, millions to billions of other spermatozoa perish. Spermatozoa are highly differentiated motile cells without synthetic capabilities. They generate energy via glycolysis and oxidative phosphorylation to sustain motility and to maintain the stability and functionality of their plasma membrane. In vivo, they spend their short lifespan bathing in female genital tract fluids of different origins, or are in vitro exposed to defined media during diverse sperm handling i.e. extension, cryopreservation, in vitro fertilization, etc. Being excitable cells, spermatozoa respond in vivo to various stimuli during pre-fertilization (capacitation, hyperactivation, oocyte location) and fertilization (acrosome reaction, interaction with the oocyte) events, mediated via diverse membrane ion-conducting channels and ligand-gated receptors. The present Thesis has mapped the presence and reactivity (sperm intactness and kinematics) of selected receptors, water and ion channels in ejaculated boar spermatozoa. The final aim was to find a relevant alternative cell type for in vitro bioassays that could ease the early scrutiny of candidate drugs as well as decreasing our needs for experimental animals according to the 3R principles. Spermatozoa are often extended, cooled and thawed to warrant their availability as fertile gametes for breeding or in vitro testing. Such manipulations stress the cells via osmotic variations and hence spermatozoa need to maintain membrane intactness by controlling the exchange of water and the common cryoprotectant glycerol, via aquaporins (AQPs). Both AQPs-7 and -9 were studied for membrane domain changes in cauda-and ejaculated spermatozoa (un-processed, extended, chilled or frozen-thawed). While AQP-9 maintained location through source and handling, thawing of ejaculated spermatozoa clearly relocated the labelling of AQP-7, thus appearing as a relevant marker for non-empirical studies of sperm cryopreservation. Alongside water, spermatozoa interact with calcium (Ca 2+ ) via the main Ca 2+ sperm channel CatSper. Increments in intracellular Ca 2+ initiate motility hyperactivation and the acrosome reaction. The four subunits of the CatSper ch...

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The CatSper calcium channel in human sperm: relation with motility and involvement in progesterone-induced acrosome reaction

Abstract: This work was supported by grants from Ministry of University and Scientific Research (Prin project to E.B. and FIRB project to S.M) and Regione Toscana (to G.F.). The authors have no conflicts of interest to declare.

Cited by 108 publications

References 50 publications

Lead Inhibits Human Sperm Functions by Reducing the Levels of Intracellular Calcium, cAMP, and Tyrosine Phosphorylation

Lead Inhibits Human Sperm Functions by Reducing the Levels of Intracellular Calcium, cAMP, and Tyrosine Phosphorylation

Ketamine inhibits human sperm function by Ca2+-related mechanism

Sperm Membrane Channels, Receptors and Kinematics : Using boar spermatozoa for drug toxicity screening

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