The CD38-independent ADP-ribosyl cyclase from mouse brain synaptosomes: a comparative study of neonate and adult brain

Ceni, Claire; Pochon, Nathalie; Villaz, Michel; Muller-Steffner, Hélène; Schuber, Francis; Baratier, Julie; Waard, Michel De; Ronjat, Michel; Moutin, Marie‐Jo

doi:10.1042/bj20051321

Cited by 29 publications

(39 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We have also evidenced robust levels of ADP-ribosyl cyclase activity in cultured PASMCs, using NGD ϩ as an alternative substrate to measure cyclic GDP ribose formation (data not shown), and have, furthermore, shown the expression of CD38 in these cells (this study). The lung is among a group of tissues containing a novel enzyme with ADPribosyl cyclase activity in addition to CD38 (61)(62)(63). This notion is supported by data from CD38 Ϫ/Ϫ mice containing an appreciable amount of cADPR in the lung albeit at lower levels than in the wild type, as well as the fact that cADPR synthesis has been measured in microsomal fraction of PASM homogenates (47,63,64).…”

Section: Discussionsupporting

confidence: 67%

Integrin Ligands Mobilize Ca2+ from Ryanodine Receptor-gated Stores and Lysosome-related Acidic Organelles in Pulmonary Arterial Smooth Muscle Cells

Umesh

Thompson

Chini

et al. 2006

Journal of Biological Chemistry

View full text Add to dashboard Cite

store, but partially blocked by ryanodine or inhibition of lysosomerelated acidic organelles with bafilomycin A1. Simultaneous inhibition of both pathways was necessary to abolish the response. GRGDSP treatment increased cyclic ADP-ribose , the endogenous activator of ryanodine receptors, by 70%. GRGDSP also rapidly reduced Lysotracker Red accumulation, confirming direct modulation of acidic organelles. These data are the first demonstration of integrin-mediated Ca 2؉ regulation in PASMCs. The presence of an array of integrins, and activation of ryanodine-sensitive Ca 2؉ stores and lysosome-like organelles by GRGDSP suggest important roles for integrin-dependent Ca 2؉ signaling in regulating PASMC function. Extracellular matrix (ECM)2 protein receptors, or integrins, comprise a superfamily of structurally related heterodimeric transmembrane receptors that mediate cell-cell and cell-ECM interactions. Integrins physically bridge the ECM and cytoskeleton, and act as transducers of "outside-in" and "inside-out" signaling (1). Extracellular integrin ligation changes [Ca 2ϩ ] i in a variety of cell types, including platelets, neutrophils, monocytes, lymphocytes, fibroblasts, endothelial cells, osteoclasts, neurons, glomerulosa cells, epithelial and smooth muscle cells (2-5). Integrin ligation also activates other intracellular signaling molecules, including H ϩ and a plethora of protein kinases such as the focal adhesion kinase, Rac, and extracellular signal regulated kinases (1, 6). As a result, a myriad of cellular functions such as differentiation, proliferation, migration, apoptosis, and mechanosensing for shear and tension are affected (6, 7).Integrins participate in controlling systemic vascular tone by invoking changes in smooth muscle [Ca 2ϩ ] i , resulting in relaxation or contraction. Synthetic ligands carrying the prototypic integrin-binding tripeptide motif, arginine-glycine-aspartate (RGD), decrease [Ca 2ϩ ] i causing vasodilation in rat cremaster muscle arterioles (8, 9). On the other hand, RGD-containing peptides constrict rat renal afferent arterioles through increased [Ca 2ϩ ] i (10, 11). Other integrin binding sequences also exist, such as leucine-aspartate-valine (LDV), which elevates [Ca 2ϩ ] i and causes vasoconstriction of rat cremaster muscle arterioles (12). Integrins ␣ 5 ␤ 1 and ␣ v ␤ 3 are, furthermore, directly involved in the vascular myogenic behavior, where blockade of either integrin inhibits myogenic constriction of skeletal muscle arterioles (13).Apart from regulating tone, integrins play an important role in vascular pathogenesis. This is evidenced by the remodeling and deposition of ECM components seen in various vascular diseases such as atherosclerosis, hypertension, and restenosis (5). In the pulmonary vasculature, remodeling occurs in chronic obstructive pulmonary disease, asthma, scleroderma, and pulmonary hypertension (14). Interestingly, pulmonary vascular remodeling has been linked to integrin activation in rat models recapitulating pulmonary hypertension. Previo...

show abstract

Section: Discussionsupporting

confidence: 67%

Integrin Ligands Mobilize Ca2+ from Ryanodine Receptor-gated Stores and Lysosome-related Acidic Organelles in Pulmonary Arterial Smooth Muscle Cells

Umesh

Thompson

Chini

et al. 2006

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…These modifications are shown to increase the recovery of cADPR, improve the functionality of the assay, and reduce between-subject variability (159) . In fact, we observed a significant reduction of brain cADPR despite a difference of only 16% between wildtype and knockout mice, in contrast to the 20% nonsignificant reduction observed by Partida-Sanchez et al (56) and the 18% non-significant reduction observed in Ceni et al (103) . We also observed that levels of NAD þ in the brain were increased by 160 % (148) (P , 0·001) ( Table 4).…”

Section: /2contrasting

confidence: 92%

“…(56) and Ceni et al (103) , who previously measured levels of cADPR in these tissues and found them to be non-significantly decreased. While our levels of brain cADPR are comparable with other published reports, the degree of variability in each group is reduced, which we believe is due to modifications that we have made to the fluorimetric cycling assay for cADPR.…”

Section: /2mentioning

confidence: 89%

The role of dietary niacin intake and the adenosine-5′-diphosphate-ribosyl cyclase enzyme CD38 in spatial learning ability: is cyclic adenosine diphosphate ribose the link between diet and behaviour?

Young

Kirkland

2008

Nutr. Res. Rev.

View full text Add to dashboard Cite

The pyridine nucleotide NAD þ is derived from dietary niacin and serves as the substrate for the synthesis of cyclic ADP-ribose (cADPR), an intracellular Ca signalling molecule that plays an important role in synaptic plasticity in the hippocampus, a region of the brain involved in spatial learning. cADPR is formed in part via the activity of the ADP-ribosyl cyclase enzyme CD38, which is widespread throughout the brain. In the present review, current evidence of the relationship between dietary niacin and behaviour is presented following investigations of the effect of niacin deficiency, pharmacological nicotinamide supplementation and CD38 gene deletion on brain nucleotides and spatial learning ability in mice and rats. In young male rats, both niacin deficiency and nicotinamide supplementation significantly altered brain NAD þ and cADPR, both of which were inversely correlated with spatial learning ability. These results were consistent across three different models of niacin deficiency (pair feeding, partially restricted feeding and niacin recovery). Similar changes in spatial learning ability were observed in Cd38 2/2 mice, which also showed decreases in brain cADPR. These findings suggest an inverse relationship between spatial learning ability, dietary niacin intake and cADPR, although a direct link between cADPR and spatial learning ability is still missing. Dietary niacin may therefore play a role in the molecular events regulating learning performance, and further investigations of niacin intake, CD38 and cADPR may help identify potential molecular targets for clinical intervention to enhance learning and prevent or reverse cognitive decline.

show abstract

“…For this, one reason may reside on cyclase species: the lower expression of CD38 in the pitutitary (Jin et al, 2007a) or contribution of other molecular species, as suggested in the embryonic brain (Ceni et al, 2006). The second possibility is utilization of different G proteins or accessory molecules.…”

Section: Discussionmentioning

confidence: 99%

Oxytocin-induced elevation of ADP-ribosyl cyclase activity, cyclic ADP-riboseor Ca2+ concentrations is involved in autoregulation of oxytocin secretionin the hypothalamus and posterior pituitary in male mice

et al. 2010

View full text Add to dashboard Cite

The CD38-independent ADP-ribosyl cyclase from mouse brain synaptosomes: a comparative study of neonate and adult brain

Cited by 29 publications

References 51 publications

Integrin Ligands Mobilize Ca2+ from Ryanodine Receptor-gated Stores and Lysosome-related Acidic Organelles in Pulmonary Arterial Smooth Muscle Cells

Integrin Ligands Mobilize Ca2+ from Ryanodine Receptor-gated Stores and Lysosome-related Acidic Organelles in Pulmonary Arterial Smooth Muscle Cells

The role of dietary niacin intake and the adenosine-5′-diphosphate-ribosyl cyclase enzyme CD38 in spatial learning ability: is cyclic adenosine diphosphate ribose the link between diet and behaviour?

Oxytocin-induced elevation of ADP-ribosyl cyclase activity, cyclic ADP-riboseor Ca2+ concentrations is involved in autoregulation of oxytocin secretionin the hypothalamus and posterior pituitary in male mice

Contact Info

Product

Resources

About