The cell cycle of the 8-cell human embryo is uniquely controlled by a novel gene set

Kiessling, Ann A.; Bletsa, Ritsa; Desmarais, B.; Mara, Christina; Kallianidis, K.; Loutradis, Dimitris

doi:10.1016/j.fertnstert.2009.07.096

Cited by 1 publication

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“…What is becoming more and more clear is that, irrespective of its origin, embryos try to react to aneuploidy, although the majority of chromosomal errors are probably unlikely to be corrected, especially if they are present in multiple copies (15). Several processes can contribute to the embryonic aneuploid self-correction, including cellular fragmentation, the formation of micronuclei, blastomere exclusion, and the presence of a stringent cell cycle control that only becomes active after the embryonic genome activation (16)(17)(18). In other words, in up to day-4 preimplantation embryos, aneuploid blastomeres can continue cell division resulting in aneuploid daughter cells.…”

Section: Discussionmentioning

confidence: 99%

Deoxyribonucleic acid detection in blastocoelic fluid: a new predictor of embryo ploidy and viable pregnancy

Magli¹,

Albanese²,

Crippa³

et al. 2019

Fertility and Sterility

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To investigate blastocysts, defined as euploid and aneuploid by trophectoderm (TE) cell analysis, for the presence of DNA in the blastocoelic fluid (BF) detected by whole-genomic amplification (WGA); and to correlate the presence of DNA in BF with the clinical outcome after the transfer of TE-euploid blastocysts. Design: Retrospective study. Setting: In vitro fertilization unit. Patient(s): This study included 91 patients performing preimplantation genetic testing for aneuploidy on TE cells from January 2015 to December 2017. In the case of ET, only single blastocyst transfers were performed. Intervention(s): Blastocoelic fluids and TE cells were retrieved from 256 blastocysts before vitrification. All blastocysts were diagnosed by array-comparative genomic hybridization (a-CGH) on TE cells. Amplification and a-CGH of DNA from BFs was performed at a later time after TE biopsy and ET. Main Outcome Measure(s): Whole-genomic amplification of BFs, evaluation of the chromosome condition in BFs and TE cells, and correlation of BF results with the clinical outcome of TE-euploid transferred blastocysts. Result(s): The incidence of amplification after WGA was significantly lower in BFs from TE-euploid blastocysts (n ¼ 32, 45%) when compared with the aneuploid ones (n ¼ 150, 81%), resulting in 182 BFs with successful DNA amplification. When submitted to a-CGH, informative results were obtained from 172 BFs. Comparison of these results with those from the corresponding TE cells gave a ploidy concordance of 93.6% and a mean number of aneuploid events per sample that was higher in BFs than in TE cells (2.0 vs. 1.4, respectively). After the transfer of 53 TE-euploid blastocysts, the clinical pregnancy rate was 77% in the group with BF-failed amplification, and 37% after BF-successful amplification. The same trend was found for the ongoing pregnancy rate (68% vs. 31.5%, respectively). Conclusion(s): The presence of DNA in BFs detected by WGA is correlated with the blastocyst ploidy condition defined by TE cell biopsy and with the implantation potential of TE-euploid blastocysts. These findings could have a clinical implication for the selection of the most viable embryo for transfer because, after submitting BFs to WGA, priority would be given to TE-euploid blastocysts with BFfailed amplification. Similarly, BF-failed amplification could be an additional selection criterion to prioritize embryos for transfer even in conventional IVF cycles with blastocysts that were vitrified after BF aspiration. (Fertil Steril Ò 2019;111:77-85. Ó2018 by American Society for Reproductive Medicine.) El resumen está disponible en Español al final del artículo.

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