The cell surface antigens of articular chondrocytes

Giant, Tibor T.; Mikecz, Katalin; Leva, Gianpiero Di

doi:10.1016/0049-0172(81)90066-4

Cited by 2 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Anti-PG antibodies bind to the surface of cartilage, primarily recognizing the GAG side chains of PG molecules in the extracellular matrix and on the chondrocyte surface (Fig. 5), where newly synthesized PG molecules are entrapped by hyaluronan, as shown by immunoelectronmicroscopy (25,45). More than 95% of anti-PG antibodies are IgG1 and IgG2a isotypes at the time of arthritis onset and can thus form immune complexes with PG molecules (33) on the cartilage surface or in the joint cavity and may subsequently activate the complement system.…”

Section: Discussionmentioning

confidence: 99%

“…Chondrocytes were isolated from the femoral head cartilage of adult naive WT BALB/c mice using pronase/collagenase digestion as previously described (25). High-density chondrocytes were cultured overnight in DMEM:F12 (50%:50%) medium containing 10% foetal bovine serum; live cells attached to the surface were washed with serum-free medium, treated with collagenase D (Roche Diagnostics GmbH, Indianapolis, IN, USA) for 30 min at 37°C and suspended in enzyme-free Cell Dissociation Buffer (Gibco/Invitrogen).…”

Section: Complement-mediated Cytotoxicity Of Mouse Chondrocytesmentioning

confidence: 99%

See 1 more Smart Citation

Arthritogenic T cells drive the recovery of autoantibody-producing B cell homeostasis and the adoptive transfer of arthritis in SCID mice

Kis-Tóth

Radács

Olasz

et al. 2012

International Immunology

Self Cite

View full text Add to dashboard Cite

T cells orchestrate joint inflammation in rheumatoid arthritis (RA), but B cells/B cell-derived factors are also involved in disease pathogenesis. The goal of this study was to understand the role of antigen-specific T and B cells in the pathological events of arthritis, which is impossible to study in humans due to the small number of antigen-specific cells. To determine the significance of antigen-specific lymphocytes and antibodies in the development of an autoimmune mouse model of RA, we generated TCR transgenic (TCR-Tg) mice specific for the dominant arthritogenic epitope of cartilage proteoglycan (PG) and performed a series of combined transfers of T cells, B cells and autoantibodies into BALB/c.Scid mice. The adoptive transfer of highly purified T cells from naive TCR-Tg, arthritic TCR-Tg or arthritic wild-type mice induced arthritis in SCID recipients, but the onset and severity of the disease were dependent on the sequential events of the T cell-supported reconstitution of PG-specific B cells and autoantibodies. The presence of activated PG-specific T cells was critical for disease induction, establishing a unique milieu for the selective homeostasis of autoantibody-producing B cells. In this permissive environment, anti-PG autoantibodies bound to cartilage and induced activation of the complement cascade, leading to irreversible cartilage destruction in affected joints. These findings may lead to a better understanding of the complex molecular and cellular mechanisms of RA.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Complement-mediated Cytotoxicity Of Mouse Chondrocytesmentioning

confidence: 99%

Arthritogenic T cells drive the recovery of autoantibody-producing B cell homeostasis and the adoptive transfer of arthritis in SCID mice

Kis-Tóth

Radács

Olasz

et al. 2012

International Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

Analysis of osteoarthritic cartilage using monoclonal antibodies reactive with rabbit proteoglycan

Kresina

Malemud

Moskowitz

1986

Arthritis & Rheumatism

View full text Add to dashboard Cite

Femoral and tibial cartilage specimens from nonoperated, sham operated, and partially meniscectomized knees of New Zealand white rabbits were stujdied, using fluorescein-conjugated mouse IgG or 3 monoclonal antibodies (2G2, 2E9, and 6C9) that portrayed differing fine antigenic specificity for rabbit cartilage proteoglycan monomer. In nonoperated and sham operated animals, monoclonal antibodies 2G2 and 2E!3 stained cellular/pericellular (C/PC) and matrix areas; antibody 6C9 stained only C/PC areas. Augmented Ch'C and matrix staining of osteoarthritic femoral cartilage occurred with 2G2 and 6C9; tibial cartilage staiining was reduced. Increased 2E9 staining of C/PC regions was seen in tibial cartilage. The differential staiining patterns indicate that proteoglycan macromoleciular changes occur in experimental osteoarthritis and that these monoclonal antibodies can be utilized as probes for the connective tissue changes observed.Osteoarthritis (OA), a progressively destructive disease of the articular surfaces of joints, is characterized by localized erosion, fibrillation of the cartilage surface, osteophyte formation, and bony remodeling (1-3). Numerous etiopathogenic variables may be involved in the generation of these pathologic changes. These include mechanical stress, aging, genetic, enzymatic, and biochemical factors, as well as an immune response to sequestered cartilage cell surface antigens and matrix components (1,4-7).The extracellular matrix of articular cartilage consists mainly of large proteoglycan (PG) molecules interspersed in a meshwork of collagen fibers. Cartilage PGs exist in the form of nonaggregated subunit monomers, and of aggregates which contain monomer, hyaluronic acid, and link glycoproteins (8,9). PG monomeric subunit molecules are approximately 1 X lo6 daltons in molecular weight, and are composed of polypeptide chain(s) with numerous extended glycosaminoglycan side chains (10). The PG molecules are characterized by their wide polydispersity and large hydrodynamic volume in solution.Numerous researchers (1 1-14) have reported a reduction in the size and proportion of aggregates, and varying capacity of cartilage proteoglycans to form aggregated macromolecules in patients with OA. In this study, we generated monoclonal antibodies specific for rabbit PG monomer subunits, for the purpose of analyzing the structural differences in proteoglycans from normal and osteoarthritic rabbit cartilage. The data suggested that PG monomers in normal and osteoarthritic femoral and tibial cartilage differ, and that monoclonal antibodies are useful probes for studying the osteoarthritic changes. MATERIALS AND METHODSPartial medial meniscectomy. Osteoarthritis was induced in rabbit knees by partial medial rneniscectorny ,

show abstract

The cell surface antigens of articular chondrocytes

Cited by 2 publications

References 2 publications

Arthritogenic T cells drive the recovery of autoantibody-producing B cell homeostasis and the adoptive transfer of arthritis in SCID mice

Arthritogenic T cells drive the recovery of autoantibody-producing B cell homeostasis and the adoptive transfer of arthritis in SCID mice

Analysis of osteoarthritic cartilage using monoclonal antibodies reactive with rabbit proteoglycan

Contact Info

Product

Resources

About