1983
DOI: 10.1159/000163184
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The Cell Surface Sulphydryl Content of Metastatic Variants of B16 Murine Melanoma

Abstract: The cell surface sulphydryl content of three metastatic variants of the B16 murine melanoma has been determined using isoelectric equilibrium techniques. The F1 variant, which has no ability for natural metastasis, and the F10 variant with moderate metastatic ability appeared to have no detectable surface thiol groups. The variant BL6, which shows a high degree of natural metastasis, possessed surface thiol groups. The variants were found to be heterogeneous in isoelectric distribution. Three subpopulations we… Show more

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Cited by 3 publications
(3 citation statements)
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“…and usually as a technical necessity, biochemical analyses have been conducted on cell monolayers while tumorigenic and metastatic properties have been assessed on single-cell suspensions prepared by either chemical, biological or mechanical dispersal of monolayer cultures (Irimura et al, 198 1 ;Raz et al, 1980;Sheppard et al, 1983Sheppard et al, , 1984Steck and Nicolson. 1983;Steiner et al, 1983) Although several investigators have employed cell suspensions for both biochemical and biological analysis (Gilbert and Gordon, 1983;LeGrue, 1982;Sherbet, 1983) in some cases the preparative methods differed (Miner et al, 1982) and in the majority of instances the cells suspended for biochemical investigations did not endure the entire spectrum of trauma experienced by parallel suspensions prepared for in vivo injection, including exposure to high shear forces by injection through a narrow-gauge needle and sudden exposure to syngeneic mouse plasma. Consequently, to ensure full complementarity between in vitro biochemical and in vivo biological assays, it is important that both be performed synchronously on replicate cell populations with identical histories.…”
Section: Discussionmentioning
confidence: 99%
“…and usually as a technical necessity, biochemical analyses have been conducted on cell monolayers while tumorigenic and metastatic properties have been assessed on single-cell suspensions prepared by either chemical, biological or mechanical dispersal of monolayer cultures (Irimura et al, 198 1 ;Raz et al, 1980;Sheppard et al, 1983Sheppard et al, , 1984Steck and Nicolson. 1983;Steiner et al, 1983) Although several investigators have employed cell suspensions for both biochemical and biological analysis (Gilbert and Gordon, 1983;LeGrue, 1982;Sherbet, 1983) in some cases the preparative methods differed (Miner et al, 1982) and in the majority of instances the cells suspended for biochemical investigations did not endure the entire spectrum of trauma experienced by parallel suspensions prepared for in vivo injection, including exposure to high shear forces by injection through a narrow-gauge needle and sudden exposure to syngeneic mouse plasma. Consequently, to ensure full complementarity between in vitro biochemical and in vivo biological assays, it is important that both be performed synchronously on replicate cell populations with identical histories.…”
Section: Discussionmentioning
confidence: 99%
“…The possibility that the negative charge on the surface of tumor cells can affect the frequency and location of metastases has been the subject of a number of different studies since the initial observation that some cancer cells have higher electrophoretic mobility and surface charge density than their normal counterparts (Ambrose et al, 1956;Purdom et al, 1958). Further studies carried out on many lines of tumor cells have shown that their metastatic potential is often correlated to high cell surface charge density (Bosmann et al, 1973;Park et al, 1982;Sherbet, 1983;Weiss, 1976). This conclusion received additional support from biochemical studies showing that some highly metastatic variants of different tumor cell lines express more sialic acid residues on the cell surface than their poorly metastatic counterparts Berthier-Vergnes et al, 1985;Bressalier et al, 1990;Collard et al, 1986;Fogel et al, 1983;Steele et al, 1983;Yogeeswaran and Tho, 1980;Yogeeswaran and Salk, 1981).…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, disturbances of the cellular thiol:disulphide balance have been associated with the multifarious changes that occur in cancer compared with the normal situation: [for reviews of the older literature see Harington (1967) and Knock et al (1967); for more recent work see Schauenstein et al (1978), and Sherbert (1983)]. Most studies quoted above were done with macroscopic or whole tissue samples thereby preventing the recognition and measurement of any highly localised disturbances that may occur in a heterogeneous lesion.…”
mentioning
confidence: 99%