The cells of monocyte-macrophage lineage in mice with the 2-month polyethylene implantation

Różalska, Barbara; Długońska, Henryka; Ljungh, Åsa; Paziak-Domańska, B.; A, Burow; Rudnicka, Wiesława

doi:10.1016/0165-2478(95)02489-1

Cited by 6 publications

(7 citation statements)

References 18 publications

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“…Rozalska et al have shown that differentiation in monocyte–macrophage cells changes upon implantation of heparinized polyethylene for 2 months. A subpopulation of immature monocytes was present in the blood of implanted mice in comparison to control mice 27. Infiltration of less mature monocytes to the implant site would be expected to impact macrophage adhesion and fusion on the material surface, but this was not observed in our study, regardless of polymer type.…”

Section: Discussioncontrasting

confidence: 57%

“…A subpopulation of immature monocytes was present in the blood of implanted mice in comparison to control mice. 27 Infiltration of less mature monocytes to the implant site would be expected to impact macrophage adhesion and fusion on the material surface, but this was not observed in our study, regardless of polymer type. In regard to material implantation possibly impacting systemic T cell populations, Huang et al has shown that implantation of multiple vascular prostheses in rats did not significantly alter the percentage of total T cells, T cell subsets, and T cells expressing IL-2 receptor.…”

Section: Discussioncontrasting

confidence: 55%

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T cell subset distributions following primary and secondary implantation at subcutaneous biomaterial implant sites

Rodriguez

Voskerician²,

Meyerson

et al. 2007

J Biomedical Materials Res

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Synthetic biomaterials are considered to be nonimmunogenic. Therefore, the role that adaptive immunity may play in the host response to implanted synthetic biomaterials has not been extensively studied. Cardinal features of adaptive immunity include specificity and T cell responses, which are greater and more effective with upregulation of activation receptors upon rechallenge. We compared the primary and secondary in vivo host response to three synthetic biomaterials: Elasthane 80A, silicone rubber, and polyethylene terephthalate using a cage implant model in Sprague Dawley rats. The synthetic biomedical polymers were subcutaneously implanted in cages for 14 days. Following explantation of the cages and a 2 week healing period, rats were implanted with cages containing the biomedical polymers for an additional 2 weeks. The cellular exudates within the cages were analyzed 4, 7, and 14 days post primary and secondary implantation by flow cytometry for the following cell types: T cells (inclusive of CD8(+), CD4(+), and CD4(+)/CD25(+) subsets), B cells, granulocytes, and macrophages. At day 14 following secondary implantation, there was an increase in T cells, granulocytes, and macrophages in the exudates when compared with primary implantation for all groups inclusive of the empty cage control. However, CD4(+)/CD8(+) ratios, the percentage of CD4(+)CD25(+) T cells, and the macrophage surface adhesion/fusion did not vary significantly upon secondary implantation. Despite a quantitative increase in T cells following secondary biomaterial exposure, T cell subset distribution did not change, indicating nonspecific recruitment rather than an adaptive immune response.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussioncontrasting

confidence: 55%

T cell subset distributions following primary and secondary implantation at subcutaneous biomaterial implant sites

Rodriguez

Voskerician²,

Meyerson

et al. 2007

J Biomedical Materials Res

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“…In previous papers we showed that long‐lasting i.p. implantation of H‐PE in mice caused changes in the differentiation of bone marrow‐derived monocytes, a reduction of the bactericidal activity of phagocytes and a decrease in the host's ability to eradicate staphylococci [5–7].…”

Section: Discussionmentioning

confidence: 99%

“…As we previously demonstrated, polymorphonuclear leukocytes and macrophages from mice implanted for 2 months with heparinized polyethylene (H‐PE) showed a marked decrease in the bactericidal activity against staphylococci [5]and Candida albicans yeasts [6]. Moreover, long‐lasting implantation caused evident changes in the differentiation of bone marrow‐derived monocytes [7]. As a consequence of this, the animals under long‐lasting implantation presented a diminished ability to eradicate bacteria, particularly those colonizing the biomaterial surface [5, 6, 8].…”

Section: Introductionmentioning

confidence: 99%

Specific immune response to staphylococcal antigens during long-lasting biomaterial implantation

Rudnicka

Sadowska

Ljungh

et al. 1997

FEMS Immunol Med Microbiol

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Biomaterial-associated infections caused by staphylococci are one of the main therapeutic problems in modern medicine. There is no doubt that local disfunction of polymorphonuclear leukocytes and macrophages predisposes to such infections. However, it is not clear how implantation of a foreign body influences the antibacterial immune response. We analyzed some parameters of the specific immune response to staphylococcal antigens, in mice implanted for 3 months with heparinized polyethylene. Three weeks before the evaluation of the immune response, mice (implanted and non-implanted) were infected i.p. with 2 x 10(7) cells of Staphylococcus aureus Cowan 1. The proliferation of splenocytes was determined on the basis of [3H]thymidine incorporation in cultures stimulated with staphylococcal lipoteichoic acid, protein A, alpha-toxin, or phytohemagglutinin. Moreover, the level of specific antibodies to staphylococcal antigens was determined in serum samples (ELISA with the antigens lipoteichoic acid, protein A, and alpha-toxin). The data obtained indicate that long-lasting implantation caused evident changes in proliferative activity of lymphocytes and in humoral response to staphylococcal antigens. It enhanced spontaneous and lipoteichoic acid- or alpha-toxin-stimulated proliferation of splenocytes, in vitro. In contrast, heparinized polyethylene-implanted animals showed a significant decrease in the production of anti-protein A IgG2b and anti-alpha-toxin IgG2a and IgG2b.

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“…Three days later, cells from the peritoneal exudate were suspended (5 X 10' cells/ml) in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS) and 2 mm L-glutamine (complete medium) supplemented with antibiotics (penicillin and streptomycin). The cells were incubated in tissue culture plates (Nunc, Kamstrup, Denmark) for 1.5 hr at 37 C in an atmosphere with 5% CO, to permit the adherence of macrophages (45). The adherent cells were resuspended in complete medium.…”

Section: Methodsmentioning

confidence: 99%

The Production of Nitric Oxide and Tumor Necrosis Factor by Murine Macrophages Infected with Mycobacterial Strains Differing by Hemolytic Activity

Rudnicka

Brzychcy

Klink³

et al. 1999

Microbiology and Immunology

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In this study, we compared the secretion of nitric oxide (NO) and tumor necrosis factor (TNF-a) by murine macrophages infected in vitro with hemolytic or unhemolytic mycobacteria isolates. We observed that unhemolytic mycobacteria induced more intensive NO production by macrophages and were more susceptible to bactericidal effect of mononuclear phagocytes than hemolytic mycobacterial strains. In contrast, the high-virulence hemolytic isolates induced significantly stronger TNF-a production by infected macrophages than the low-virulence unhemolytic bacilli.

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The cells of monocyte-macrophage lineage in mice with the 2-month polyethylene implantation

Cited by 6 publications

References 18 publications

T cell subset distributions following primary and secondary implantation at subcutaneous biomaterial implant sites

T cell subset distributions following primary and secondary implantation at subcutaneous biomaterial implant sites

Specific immune response to staphylococcal antigens during long-lasting biomaterial implantation

The Production of Nitric Oxide and Tumor Necrosis Factor by Murine Macrophages Infected with Mycobacterial Strains Differing by Hemolytic Activity

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