The Cellular Basis of Extreme Alkali Secretion in Insects: A Tale of Two Tissues

Moffett, David F.; Onken, Horst

doi:10.1007/978-1-60327-229-2_5

Cited by 3 publications

(3 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cuticular proteins are involved in forming the insect cuticle, and their upregulation promotes resistance by altering insecticide penetration [27][28][29]. Sodium/hydrogen exchanger and synaptotagmin-1 are associated with neuronal functions and synaptic vesicle release, indicating their potential involvement in neurophysiological changes associated with resistance [30,31]. Synaptotagmin-1 is used as a target in a yeast-interfering RNA larvicide for controlling disease vector mosquitoes, indicating that it plays a role in resistance [32].…”

Section: Discussionmentioning

confidence: 99%

Key gene modules and hub genes associated with pyrethroid and organophosphate resistance in Anopheles mosquitoes: A systems biology approach

Odhiambo,

Derilus,

Impoinvil

et al. 2024

Preprint

View full text Add to dashboard Cite

Indoor residual spraying (IRS) and insecticide-treated nets (ITNs) are the main methods used to control mosquito populations for malaria prevention. The efficacy of these strategies is threatened by the spread of insecticide resistance (IR), limiting the success of malaria control. Studies of the genetic evolution leading to insecticide resistance could enable the identification of molecular markers that can be used for IR surveillance and an improved understanding of the molecular mechanisms associated with IR. This study used a weighted gene co-expression network analysis (WGCNA) algorithm, a systems biology approach, to identify genes with similar co-expression patterns (modules) and hub genes that are potential molecular markers for insecticide resistance surveillance in Kenya and Benin. A total of 20 and 26 gene co-expression modules were identified via the average linkage hierarchical clustering from Anopheles arabiensis and An. gambiae, respectively, and hub genes (highly connected genes) identified within each module. Four specific genes stood out: serine protease, E3 ubiquitin-protein ligase, cuticular protein RR2, and leucine-rich immune protein, which were top hub genes in both species and could serve as potential markers and targets for monitoring IR in these malaria vectors. In addition to the identified markers, we explored molecular mechanisms using enrichment maps that revealed a complex process involving multiple steps, from odorant binding and neuronal signaling to cellular responses, immune modulation, cellular metabolism, and gene regulation. Incorporation of these dynamics into the development of new insecticides and tracking of insecticide resistance could improve sustainable and cost-effective deployment of interventions.

show abstract

Section: Discussionmentioning

confidence: 99%

Key gene modules and hub genes associated with pyrethroid and organophosphate resistance in Anopheles mosquitoes: A systems biology approach

Odhiambo,

Derilus,

Impoinvil

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Cuticular proteins form the insect cuticle, and their upregulation promotes resistance by altering insecticide penetration [ 31 – 33 ]. Sodium/hydrogen exchangers and synaptotagmin-1 are associated with neuronal functions and synaptic vesicle release, indicating their potential involvement in neurophysiological changes associated with resistance [ 34 , 35 ]. Synaptotagmin-1 is used as a target in a yeast-interfering RNA larvicide for controlling disease vector mosquitoes, indicating that it plays a role in resistance [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Key gene modules and hub genes associated with pyrethroid and organophosphate resistance in Anopheles mosquitoes: a systems biology approach

Odhiambo,

Derilus,

Impoinvil

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Indoor residual spraying (IRS) and insecticide-treated nets (ITNs) are the main methods used to control mosquito populations for malaria prevention. The efficacy of these strategies is threatened by the spread of insecticide resistance (IR), limiting the success of malaria control. Studies of the genetic evolution leading to insecticide resistance could enable the identification of molecular markers that can be used for IR surveillance and an improved understanding of the molecular mechanisms associated with IR. This study used a weighted gene co-expression network analysis (WGCNA) algorithm, a systems biology approach, to identify genes with similar co-expression patterns (modules) and hub genes that are potential molecular markers for insecticide resistance surveillance in Kenya and Benin. A total of 20 and 26 gene co-expression modules were identified via average linkage hierarchical clustering from Anopheles arabiensis and An. gambiae, respectively, and hub genes (highly connected genes) were identified within each module. Three specific genes stood out: serine protease, E3 ubiquitin-protein ligase, and cuticular proteins, which were top hub genes in both species and could serve as potential markers and targets for monitoring IR in these malaria vectors. In addition to the identified markers, we explored molecular mechanisms using enrichment maps that revealed a complex process involving multiple steps, from odorant binding and neuronal signaling to cellular responses, immune modulation, cellular metabolism, and gene regulation. Incorporation of these dynamics into the development of new insecticides and the tracking of insecticide resistance could improve the sustainable and cost-effective deployment of interventions.

show abstract

“…Furthermore, it has been suggested that V-ATPases are involved in potassium ion transport in many insect biological systems, which causes alkalinity (Moffett and Onken, 2010;Wieczorek et al, 1991Wieczorek et al, , 2000Wieczorek, 1992;Onken et al, 2008). The physiological basis of alkalinity in the gut lumen of the mixed segment of higher termites has yet to be elucidated, but increased concentrations of potassium and carbonate ions have been found in the anterior hindgut of soil-feeding higher termites, suggesting the secretion of alkaline ions from the mesenteric tissue of the mixed segment (Bignell et al, 1983;Kappler and Brune, 1999).…”

Section: Discussionmentioning

confidence: 99%

Predominant expression and activity of vacuolar H+-ATPases in the mixed segment of the wood-feeding termite Nasutitermes takasagoensis

Kumara

Saitoh

Aoyama

et al. 2015

Journal of Insect Physiology

View full text Add to dashboard Cite

The Cellular Basis of Extreme Alkali Secretion in Insects: A Tale of Two Tissues

Cited by 3 publications

References 58 publications

Key gene modules and hub genes associated with pyrethroid and organophosphate resistance in Anopheles mosquitoes: A systems biology approach

Key gene modules and hub genes associated with pyrethroid and organophosphate resistance in Anopheles mosquitoes: A systems biology approach

Key gene modules and hub genes associated with pyrethroid and organophosphate resistance in Anopheles mosquitoes: a systems biology approach

Predominant expression and activity of vacuolar H+-ATPases in the mixed segment of the wood-feeding termite Nasutitermes takasagoensis

Contact Info

Product

Resources

About