The cellular content of non Hodgkin lymphomas: A comprehensive analysis using monoclonal antibodies and other surface marker techniques

Habeshaw, J. A.; Bailey, Denis; Stansfeld, A. G.; Greaves, Melvyn F.

doi:10.1038/bjc.1983.52

Cited by 66 publications

(17 citation statements)

References 50 publications

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“…In one other study of 23 lymph nodes and seven spleens the proportion of E rosette forming (T) cells was higher in nodes or spleens prior to therapy; however, no statistical comparisons were performed (Jaffe et al, 1977). This study, does not provide an explanation for the wide variation in the proportion of T cells in follicular lymphomas as found here and in many previous studies (Jaffe et al, 1974;Habeshaw et al, 1983;Harris and Bhan, 1983;Knowles et al, 1984;Swerdlow et al, 1985). Furthermore, it does not explain the reason why the proportions of T cells may change over time in the nodes from individual patients.…”

Section: Discussioncontrasting

confidence: 77%

T cells in follicular centroblastic/centrocytic (cleaved follicular centre cell) lymphomas at diagnosis, relapse and transformation

Swerdlow

Habeshaw

Murray

et al. 1989

Hematological Oncology

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Although T cells are often a prominent component of follicular (+/- diffuse) centroblastic/centrocytic (cleaved follicular centre cell--FCC) lymphomas (CB/cc/F +/- D), their evolution during the course of the disease and prognostic significance are uncertain. A total of 127 histopathologic and immunophenotypic studies from 77 patients with at least one histologic diagnosis of CB/cc/F +/- D were therefore retrospectively reviewed to address these questions. The proportion of T cells in each biopsy was analysed with reference to both histologic (diagnosis--Kiel and Lukes/Collins classification, presence of transformation, growth pattern, presence of residual mantle zones, biopsy sites) and clinical (biopsy date and number, treatment status) parameters. The actuarial survival for those patients whose first or second biopsy showed CB/cc/F +/- D and had immunophenotypic studies was also compared based on the proportions of T cells present. Among the biopsies which were obtained from 0 to 153 months following diagnosis, the mean proportion of T cells was 25 +/- 18 per cent. The only histopathologic group that showed a significantly different proportion of T cells were lymphomas of large cleaved FCC type (38 +/- 20 per cent). Although the number of T cells present varied greatly between different patients and among different biopsies in the same patients, neither biopsy number nor treatment status affected the mean proportion of T cells in CB/cc/F +/- D. With the exception of those biopsies from 1 to 11 months following diagnosis, the interval time between diagnosis and biopsy also did not affect the number of T cells present. Finally, the proportion of T cells in the lymphomas could not be shown to be of prognostic importance. Thus, the proportion of T cells in de novo CB/cc/F +/- D, at relapse or in transformation does not vary in a consistent fashion. Although extremely variable, the number of T cells present cannot be considered a feature of earlier or later disease and is not of demonstrable prognostic importance. Studies of specific subsets of T cells may provide more definite associations.

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Section: Discussioncontrasting

confidence: 77%

T cells in follicular centroblastic/centrocytic (cleaved follicular centre cell) lymphomas at diagnosis, relapse and transformation

Swerdlow

Habeshaw

Murray

et al. 1989

Hematological Oncology

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“…The helper predominance was demonstrated previously in reactive lymph nodes, tonsils, spleens, and low-grade malignant lymphomas (Dvoretsky et al, 1982;Kvaloy et al, 1982;Aisenberg et al, 1983;Habeshaw et al, 1983a;Porwit-Ksiazek et al, 1983;Si et al, 1983). However, in normal tissues, the helperhuppressor ratio was usually higher (2.5-2.9; Kvaloy et al, Habeshaw et al, 1983a;Porwit-Ksiazek et al, 1983) than in highly malignant lymphomas under study (2.0).…”

Section: Preliminary Clinical Observationsmentioning

confidence: 65%

“…However, in normal tissues, the helperhuppressor ratio was usually higher (2.5-2.9; Kvaloy et al, Habeshaw et al, 1983a;Porwit-Ksiazek et al, 1983) than in highly malignant lymphomas under study (2.0). In other reports (Kvaloy et al, 1982; Aisenberg et al, 1983;Habeshaw et al, 1983a;Lindemalm et al, 19836) concerning both low-grade and high-grade NHL, cases of B-cell lymphomas in which T-suppressors were more frequent among infiltrating T cells were observed. In our material, selected for highly malignant lymphomas, T-helperhducer predominance was the constant feature.…”

Section: Preliminary Clinical Observationsmentioning

confidence: 94%

Characterization of malignant and non‐neoplastic cell phenotypes in highly malignant non‐Hodgkin lymphomas

Porwit-Ksiazek

Christensson

Lindemalm

et al. 1983

Intl Journal of Cancer

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Malignant and non-neoplastic cells in 38 cases of highly malignant non-Hodgkin lymphomas: 3 centrocytic anaplastic, 18 centroblastic, 13 immunoblastic and 4 lymphoblastic (according to the Kiel classification) were immunophenotyped in cryosections and cell suspensions by means of monoclonal antibodies. Additionally, cell cycle analysis on cell suspensions was performed by DNA flow cytofluorometry. In 33 (87%) lymphomas the malignant cells expressed monoclonal surface immunoglobulin (Ig), which indicated B-cell origin of tumors. In 7 of the 19 B-cell lymphomas tested by the peroxidase-antiperoxidase method, cytoplasmic Ig was found. Four lymphomas were of T-cell and one of non-B/non-T-cell origin. In II B-cell and 2 lymphoblastic non-B-cell tumors, common acute lymphoblastic leukemia antigen (CALLA) was found. In 25 of 30 studied NHL the malignant cells expressed receptor for transferrin and in 19 of 28 cases a high percentage of cells in S-phase (greater than 10.85%) was found. Number and distribution as well as type of non-B-cells infiltrating B-cell-derived lymphomas varied considerably from case to case. Among these cells Leu 3+ (T helper/inducer) cells predominated. Leu 2+ (T suppressor/cytotoxic) and Leu 7+ (natural killer and killer) cells constituted less numerous groups. Correlation of cytodiagnostic analyses with clinical observations indicates that high content of infiltrating T cells may be a favorable prognostic feature in highly malignant B-cell lymphomas.

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“…negates many of the criticisms associated with cell selection or tumour loss during cell preparations that may be inherent in earlier studies of cell suspensions (Payne et al, 1977;Habershaw et al, 1983). While we find good agreement between the two approaches section analysis was particularly valuable for the characterization of T cell and histiocytic tumours.…”

Section: B Cell Lymphomasmentioning

confidence: 70%

Correlation between histology and immunophenotype in a series of 322 cases of non‐Hodgkin's lymphoma

Smith

Jones

Bell

et al. 1989

Hematological Oncology

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The non-Hodgkin's lymphomas (NHL) are a heterogeneous group of lymphoid neoplasms displaying a wide variation in cell morphology, histological patterns, immunological phenotype and prognosis. In this paper we compare the results of phenotypic investigation of 322 tissue biopsies with the histology based on the Kiel classification. Immunological analysis revealed that 81 per cent of these tumours were of B cell origin, 12 per cent of T cell origin and the remaining 7 per cent could not be characterized as representing either cell lineage. This last group included a number of cases which had received a histological diagnosis of true histiocytic lymphoma. The original morphological diagnosis, based on routine haematoxylin and eosion sections correlated with the immunologically determined phenotype in 86 and 93 per cent of the T- and B-cell cases respectively. The B cell tumours were phenotypically heterogenous with respect to immunoglobulin (Ig) heavy chain and B lymphocyte subset marker expression. IgG was most often found associated with NHL of cb/cc histology and a small subgroup of lymphocytic NHL. IgA expression was uncommon and occurred in combination with IgD and G in three cases and alone in two cases of NHL. The most common immunoglobulin isotype expressed was IgM this isotype occurred with IgD most often in lymphocytic and centrocytic NHL and less often in tumours of cb/cc histology. Whilst greater than 90 per cent of the lymphocytic NHLs expressed the CD5 antigen, between 20 and 75 per cent of B-cell tumours of other histologies also expressed this epitope. The CD10 antigen and the epitope recognized by the monoclonal reagent FMC7 were widely distributed on tumour cells from all histologies. TdT expression commonly regarded as a marker for immature cells was found in one case of follicle centre cell lymphoma. All cases of T cell NHL displayed marked heterogeneity for both pan T and T subset antigens which is significant in terms of the routine diagnosis of T NHL and with regard to the rational classification of node based T NHL. Unlike resting peripheral blood T cells, MHC class II, OKT 10 and CD25 epitopes were expressed reflecting activation of tumour populations.(ABSTRACT TRUNCATED AT 400 WORDS)

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The cellular content of non Hodgkin lymphomas: A comprehensive analysis using monoclonal antibodies and other surface marker techniques

Cited by 66 publications

References 50 publications

T cells in follicular centroblastic/centrocytic (cleaved follicular centre cell) lymphomas at diagnosis, relapse and transformation

T cells in follicular centroblastic/centrocytic (cleaved follicular centre cell) lymphomas at diagnosis, relapse and transformation

Characterization of malignant and non‐neoplastic cell phenotypes in highly malignant non‐Hodgkin lymphomas

Correlation between histology and immunophenotype in a series of 322 cases of non‐Hodgkin's lymphoma

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