The Cellular Diversity and Transcription Factor Code of Drosophila Enteroendocrine Cells

Guo, Xingting; Yin, Chang; Fu, Yang; Zhang, Yongchao; Huang, Huanwei; Wang, Jiawen; Deng, Bowen; Cai, Tao; Rao, Yi; Xi, Rongwen

doi:10.1016/j.celrep.2019.11.048

Cited by 107 publications

(164 citation statements)

References 56 publications

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“…Strategies include appending bulky or electron-withdrawing substituents, 1,4 or including heteroatoms into the core acene unit, such as in the synthesis of anthradithiophenes or azaacenes. 1,[5][6][7][8][9][10][11][12][13] Other efforts explore a redesign of the bonding architecture of the core molecule, such as through the incorporation of formally antiaromatic cyclobutadiene units, present in [N]phenylenes and phenylenecontaining oligoacenes (POAs). [14][15][16][17][18][19] Alternation of cyclobutadiene units with either benzene ([N]phenylenes) or acene units (POAs) results in localized conjugation along the molecular backbone, which can improve chromophore stability.…”

Section: Introductionmentioning

confidence: 99%

Naphthazarin-Polycyclic Conjugated Hydrocarbons and Iptycenes

et al. 2017

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The synthesis of a set of naphthazarin-containing polycyclic conjugated hydrocarbons is described herein. Sequential Diels-Alder reactions on a tautomerized naphthazarin core were employed to access the final conjugated systems. Complete conjugation across the backbone can be achieved through complexation with BF, as observed by H NMR analysis and UV/vis spectroscopy. Precisesynthetic control over the degree of oxidation of naphthazarin quinone Diels-Alder adduct 10 is additionally demonstrated and enables us to direct its subsequent reactivity. Finally, this work serves to demonstrate the potential for naphthazarin as a building block in the synthesis of novel organic electronic materials.

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Section: Introductionmentioning

confidence: 99%

Naphthazarin-Polycyclic Conjugated Hydrocarbons and Iptycenes

et al. 2017

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“…Next, reads were aligned to the D. melanogaster and virus genomes to obtain gene/viral counts for each cell, which were used to cluster generation. All clusters were identified based on a set of marker genes (Guo et al, 2019; Figure S1A). In total, six, 67 and 794 cells infected with DCV, DMelNV, and TV, respectively, were found.…”

Section: Detection Of Rna Viruses In Public D Melanogaster Scrna-seqmentioning

confidence: 99%

“…Recently, ten EE subtypes were identified in the fruit fly Drosophila melanogaster Meigen. These subtypes can be divided in two major classes: class I is composed by cells expressing AstC, and class II is composed by cells that express Tk (Beehler-Evans and Micchelli, 2015;Guo et al, 2019). In addition to these classes, a subtype dubbed III that does not express neither AstC nor Tk was also identified.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to these classes, a subtype dubbed III that does not express neither AstC nor Tk was also identified. Further classification of EE subtypes is based on whether they are located in the anterior, medium (gastric region) or posterior regions of the gastrointestinal tract (-a, -m and -p suffixes; Figure 1A) and on their gene expression (Guo et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Single-cell RNA-sequencing (scRNA-seq) allows for the analysis of subtle and cell (sub)typedependent alterations of gene expression that would otherwise be undetected in bulk RNA-seq experiments. Here, cells infected with picorna-like viruses from a publicly available fly EEs scRNA-seq dataset (Guo et al, 2019) were used to investigate the infection dynamics of two viruses, DMelNV and the recently discovered Thika virus (TV; unclassified picorna-like virus), on different subtypes of EEs. Since these viruses are polyadenylated, we have been able of quantifying their replication level based on their number of unique molecular identifiers (UMI).…”

Section: Introductionmentioning

confidence: 99%

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Heterogeneity in the response of different subtypes ofDrosophila melanogasterenteroendocrine cells to viral infections

Silva

Nagata

Melo

et al. 2020

Preprint

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SummarySingle cell RNA sequencing (scRNA-seq) offers the possibility to monitor both host and pathogens transcriptomes at the cellular level. Here, public scRNA-seq data from Drosophila melanogaster have been used to compare the differences in replication strategy and cellular response between two viruses, Thika virus (TV) and D. melanogaster Nora virus (DMelNV) in enteroendocrine cells (EEs). TV and DMelNV exhibited different patterns of replication and for TV, accumulation varied according to cell subtype. Cells infected with TV underwent down-regulation of genes that represent bottlenecks in the fruit fly interactome, while cells infected with DMelNV went through a down-expression of translation-related genes that represent both hubs and bottlenecks in the host interactome. In contrast, flies infected with DMelNV show only a systemic level down-regulation of bottleneck genes. Here, we use scRNA-seq to highlight the differences and commonalities between cellular response to TV and DMelNV and between cellular and systemic response to DMelNV.

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Liquid Metal Microscale Deposition enabled High Resolution and Density Epidermal Microheater for Localized Ectopic Expression in Drosophila

Wang

Gao

Jiang

et al. 2021

Adv Materials Technologies

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Localized ectopic expression of enteroendocrine cells in fruit flies provides a convenient model for studying human intestinal diseases, e.g., inflammatory bowel disease, that need urgent attention. Due to its noninvasive feature, thermal activating ectopic expression is an excellent option, but it demands local heating of the tiny and delicate live flies’ abdomen (≈1 × 1 mm2) without blocking their breath, instead of global heating of the whole body. This work proposes a thin, high‐resolution, and density liquid metal (LM) microheater with gas permeability and sufficient compliance to provide homogenous local heating to the fly's tiny abdomen. A depositing technique is developed that can make microscale thin (5 µm), smooth (Sa = 0.8 µm), gas permeable LM film for such microheater by introducing LM micrometer/sub‐micrometer particles. Collision induced LM particle merging and hence sintering is proposed to solve low conductivity due to their particular shells of micrometer/sub‐micrometer particles. The deposition can conformally print on the wavy or rough surface due to the fine particle size. With a shadow mask, this microscale deposition can enable high yield and reproducible thin LM patterning with high resolution, density, and precision simultaneously. Furthermore, it is demonstrated that epidermal patches can compliantly attach onto the wrinkle skin and fingerprint. Finally, by applying such a microheater on the fly abdomen, localized ectopic expression of enteroendocrine cells is successfully demonstrated.

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The Cellular Diversity and Transcription Factor Code of Drosophila Enteroendocrine Cells

Cited by 107 publications

References 56 publications

Naphthazarin-Polycyclic Conjugated Hydrocarbons and Iptycenes

Naphthazarin-Polycyclic Conjugated Hydrocarbons and Iptycenes

Heterogeneity in the response of different subtypes ofDrosophila melanogasterenteroendocrine cells to viral infections

Liquid Metal Microscale Deposition enabled High Resolution and Density Epidermal Microheater for Localized Ectopic Expression in Drosophila

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