The cellular envelope of oocytes in teleosts

Abraham, M.; Hilge, Volker; Lison, Shoshana; Tibika, Hélène

doi:10.1007/bf00217866

Cited by 43 publications

(22 citation statements)

References 24 publications

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“…4B,E,H) correspond to the same interstitial compartment in which prethecal cells are observed. It is delimited by a basement membrane and has direct connections with the pericapillary spaces (Abraham et al, 1984). In the later stages of ovarian development, this compartment is referred to as the extravascular space (Fig.…”

Section: Discussionmentioning

confidence: 99%

Gonadal development and sex differentiation in the cichlid fishCichlasoma dimerus (Teleostei, perciformes): A light- and electron-microscopic study

2005

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Although the overall pattern and timing of gonadal sex differentiation have been established in a considerable number of teleosts, the ultrastructure of early stages of gonadal development is not well documented. In this study, gonads from larval and juvenile stages of laboratory-reared Cichlasoma dimerus were examined at the light-microscopic and ultrastructural levels. This freshwater species adapts easily to captivity and spawns with high frequency during 8 months of the year, providing an appropriate model for developmental studies. Larvae and juveniles were kept at a water temperature of 26.5 +/- 1 degrees C and a 12:12 hour photoperiod. Gonadal development was documented from 14-100 days postfertilization, covering the period of histologically discernible sex differentiation. Gonadal tissue was processed according to standard techniques for light and electron microscopy. C. dimerus, a perciform teleost, is classified as a differentiated gonochorist, in which an indifferent gonad develops directly into a testis or ovary. On day 14, the gonadal primordium consists of a few germ cells surrounded by enveloping somatic cells. Ovarian differentiation precedes testicular differentiation, as usual in teleost fishes. The earliest signs of differentiation, detected from day 42 onward, include the onset of meiotic activity in newly formed oocytes, which is soon accompanied by increased oogonial mitotic proliferation and the somatic reorganization of the presumptive ovary. The ovarian cavity is completely formed by day 65. Numerous follicles containing perinucleolar oocytes are observed by day 100. In contrast, signs of morphological differentiation in the presumptive testis are not observed until day 72. By day 100, the unrestricted lobular organization of the testis is evident. The latest stage of spermatogenesis observed by this time of testicular development is spermatocyte II.

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Section: Discussionmentioning

confidence: 99%

Gonadal development and sex differentiation in the cichlid fishCichlasoma dimerus (Teleostei, perciformes): A light- and electron-microscopic study

2005

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“…In X. gladias oocytes, Vg was detected in yolk granules and intergranular cytoplasm but not in the follicular envelope. According to Abraham et al (1984) the follicular cells are not involved directly in the transfer of exogenous material into the oocytes. However, in other fish species Vg was present in the follicular envelope (Susca et al, 2001;Sarasquete et al, 2002 a, b).…”

Section: Discussionmentioning

confidence: 99%

Caracterización histoquímica de ovocitos del pez espada, <i>Xiphias gladius</i>

Ortiz-Delgado¹,

Porcelloni²,

Fossi³

et al. 2008

Sci. Mar.

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SUMMARY: This paper reports a histological, histochemical and immunohistochemical characterisation of growing oocytes of the swordfish Xiphias gladius. The presence and distribution of carbohydrates, proteins, lipids, calcium, iron, vitellogenin/ Vg, zona radiata protein/Zrp, metallothionein/Mt, and thyroid hormones/T 3 -T 4 were studied during oogenesis (cortical alveoli, globules, yolk-granules, cytoplasm, follicular and radiata envelopes). During the initial vitellogenic phase, the oocytes showed cortical alveoli and oil globules containing neutral lipids exclusively. During this phase, small yolk granules appeared around the peripheral cytoplasm, and they increased through exogenous vitellogenesis. Yolk granules were composed of glycoproteins, calcium, iron, and proteins rich in lysine, arginine, tyrosine, tryptophan, cysteine and cystine. Vg and Mt were immunohistochemically detected in yolk. The follicular envelope contained proteins rich in amino acids. Moreover, calcium and thyroid hormones (triiodotyronine and thyroxine/T 3 , T 4 ) were detected in this cell envelope. Cortical alveoli, which contained carboxylated and neutral glycoconjugates, were especially rich in N-acetyl-D-galactosamine, N-acetyl-Dglucosamine, galactose and sialic acid. Finally, the zona radiata was mainly proteinaceous in nature and was composed of calcium and neutral glycoproteins. The egg envelope or chorion and the liver showed specific immunoreactivities by using anti-salmon Zrp as the primary antiserum.Keywords: histochemistry, carbohydrates, lipids, glycoconjugates, vitellogenin, zona radiata protein, methallothionein, thyroid homones, cations, oocytes, Xiphias gladius. RESUMEN: Caracterización histoquímica de ovocitos del pez espada, Xiphias gladius. -En este trabajo se realiza una caracterización histológica, histoquímica e inmunohistoquímica de los ovocitos en crecimiento del pez espada, Xiphias gladius. Se estudia la presencia y distribución de carbohidratos, proteínas, lípidos, calcio, hierro, vitelogenina/Vg, proteína radiada/Zrp, metalotioneina/Mt y hormonas tiroideas/ T 3 -T 4 durante la ovogénesis (alveolos corticales, glóbulos, gránulos-vitelo, citoplasma, capas folicular y radiada). Durante la fase inicial de la vitelogénesis, los ovocitos presentan alveolos corticales y una gota lipídica conteniendo exclusivamente lípidos neutros. Durante esta fase en la periferia del citoplasma aparecen pequeños granulos de vitelo, incrementando progresivamente durante la vitelogénesis exógena. Los gránulos de vitelo están compuestos por glicoproteínas, calcio, hierro, proteínas ricas en lisina, arginina, tirosina, triptófano, cisteina y cistina, y contienen Vg y Mt. La capa folicular de los ovocitos del pez espada está compuesta por glicoproteínas y proteínas ricas en diferentes aminoácidos. Esta envuelta contiene calcio y hormonas tiroideas/ T 3 y T 4 . Los alveolos corticales están constituidos por glicoconjugados carboxilados y neutros, y son especialmente ricos en N-acetil-D-galactosamina, N-acetil-D-glucosamina, galactosa y...

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“…lla) (Abraham et al, 1984;Brummett and Dumont, 1977;Dumont, 1978;Wallace, 1982,1983). Circulating materials appear to leave the perifollicular capillaries both by intracellular (pinocytotic) transport across and by passage between adjacent endothelial cells.…”

Section: Vitellogenesismentioning

confidence: 98%

Ultrastructural aspects of oogenesis and oocyte growth in fish and amphibians

Wallace

Selman

1990

J. Elec. Microsc. Tech.

235

152

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Oogenesis, the early events of primary oocyte growth (meiotic arrest, synapsis, ribosomal gene duplication), and folliculogenesis can be seen to particular advantage in the germinal ridge of the syngnathan ovary. After budding off the germinal ridge (a compartment of the luminal epithelium), nascent follicles then enter into a linear array of developing follicles within which temporal and stage-specific events can be correlated with spatial distribution. Prominent features of the later phase of primary oocyte growth include intense transcriptional activity and the formation and subsequent dispersal of the Balbiani vitelline body (mitochondrial cloud) concomitant with an increase in cytoplasmic organelles and volume. Further oocyte growth is characterized by a period of cortical alveolus (in teleosts) or cortical granule (in anurans) formation, in which Golgi elements play a predominant role, and finally vitellogenesis. The latter process, which is responsible for the preponderance of oocyte growth, includes the hepatic synthesis and secretion of vitellogenin (VTG), the uptake of VTG from the bloodstream into the oocyte by receptor-mediated endocytosis, and the transport of VTG via endosomes and multivesicular bodies to forming yolk platelets. In the process, VTG is proteolytically cleaved into the yolk proteins, which assume either a monoclinic (in cyclostomes) or orthorhombic (in teleosts and amphibians) crystalline array. Other structures associated with the growing oocyte are also briefly discussed, including nuage, the vitelline envelope, intercellular junctions between the oocyte and overlying follicle cells, pigment, intramitochondrial crystals in ranidae, and annulate lamellae.

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The cellular envelope of oocytes in teleosts

Cited by 43 publications

References 24 publications

Gonadal development and sex differentiation in the cichlid fishCichlasoma dimerus (Teleostei, perciformes): A light- and electron-microscopic study

Gonadal development and sex differentiation in the cichlid fishCichlasoma dimerus (Teleostei, perciformes): A light- and electron-microscopic study

Caracterización histoquímica de ovocitos del pez espada, <i>Xiphias gladius</i>

Ultrastructural aspects of oogenesis and oocyte growth in fish and amphibians

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