The cellular events associated with regression and progression of murine (moloney) sarcomas

Russell, Stephen W.; Cochrane, Charles G.

doi:10.1002/ijc.2910130107

Cited by 32 publications

(10 citation statements)

References 10 publications

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“…A cell culture line of Moloney virus-transformed cells (MSC), transplanted to syngeneic mice, was investigated by Russell and associates (42)(43)(44)(45). This cell line was rejected by many syngenetic hosts.…”

Section: Leucocytic Infiltration In Animal Tumorsmentioning

confidence: 99%

Relationship of tumor leucocytic infiltration to host defense mechanisms and prognosis

1984

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The interface between the tumor and the host is often the site of leucocytic infiltration. We will examine the idea that the infiltrating leucocytes of human and experimental tumors are components of the host immunological defense against the tumor, and that the presence of the infiltrate is a marker of favorable prognosis. Leucocytes could infiltrate tumors because of an active immune response, either nonspecific or specifically directed to tumor-associated antigens. Leucocyte influx may also occur because of chemotactic factors secreted by the tumor cells. Some tumors release factors which enhance vascular permeability and permit improved access by leucocytes to the tumor focus. The consequences of leucocytic infiltration include tumor cell cytolysis, cytostasis, or stimulation of proliferation. The present state of our knowledge of the interactions between tumor cells and infiltrating leucocytes precludes broad generalization of mechanisms. Further study will probably reveal that the mechanisms are diverse, and that there are some systems in which immune interactions occur at this interface and others in which they do not.

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Section: Leucocytic Infiltration In Animal Tumorsmentioning

confidence: 99%

Relationship of tumor leucocytic infiltration to host defense mechanisms and prognosis

1984

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“…The DBA/2 niastocytoma, P815, was employed to monitor nonspecific cytolysis in the release assay. The in vitro techniques for maintenance and harvest of these cell lines, as well as their growth characteristics, have been described (Russell and Cochrane, 1974;Gillespie et al, 1977).…”

Section: Cell Linesmentioning

confidence: 99%

Development and persistence of cytolytic T lymphocytes in regressing or progressing moloney sarcomas

Gillespie

Russell

1978

Intl Journal of Cancer

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Intratumoral T lymphocytes were recovered sequentially after induction of regressing or progressing Moloney sarcomas in BALB/c mice and were assayed quantitatively for their ability to kill specifically the tumor (MSC) cells used for induction. The cytolytic activities of the two lymphocyte populations described two distinct biphasic kinetic profiles that were similar in amplitude and duration but separated from each other by 4-6 days. In progressing neoplasm, there was a rapidly occuring accumulation of T lymphocytes highly cytolytic for MSC cells. This response, however, was not sustained and disappeared in association with the onset of unchecked tumor growth. In contrast, T lymphocyte cytolytic activity developed more slowly in regressing sarcomas and attained peak levels coincident with the beginning of tumor regression. Similar changes in cytolytic activity characterized T lymphocytes in lymph nodes draining tumors. When cultured in vitro for 4 days, non-cytotoxic T lymphocytes from regional lymph nodes draining progressing sarcomas regained very high levels of cytolytic activity. Such restitution was diminished, however, if MSC cell lysates, macrophages or macrophages fed MSC cell lysates were present during the culture period. These experiments provided presumptive evidence that T lymphocyte-mediated cytolytic activity was lost in progressively growing Moloney sarcomas as a consequence of suppression in vivo of the genesis and/or functional expression of cytolytic T lymphocytes, perhaps by macrophages and/or soluble tumor antigen.

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“…LNC suspensions depleted of MO before culture mediated levels of tumour-specific killing (43 and 55 LU/106 T cells after 2 and 4 days, respectively) that were comparable to those of LNC (Russell and Cochrane, 1974). Tumour size increased roughly linearly in mice that received either physiological saline or cultured, nonimmune LNC.…”

Section: Effect Of Culture Conditions On Cytolytic Activitymentioning

confidence: 89%

Resurgence of killing and in vivo protection mediated by lymphocytes cultured from lymph nodes draining Moloney sarcomas

Gillespie¹,

Hansen²,

Russell³

1978

Br J Cancer

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Summary.-We have previously documented the development and subsequent disappearance of cytolytic activity mediated by lymphocytes from lymph nodes draining Moloney sarcomas destined either to regress or grow progressively. We now report that these tumour-draining lymphnode cells (LNC) that were no longer cytotoxic, spontaneously regenerated peak levels of killing after culture in vitro for 4 days in the absence of exogenous tumour antigen. Cytolytic activity, which was antigenically specific, was mediated by T lymphocytes. Resurgence of cytolytic activity in vitro was accompanied by proliferative changes (DNA synthesis, blast transformation, cell division) which peaked on the 3rd day of culture. Although normal, nonimmune LNC underwent quantitatively similar proliferative changes in culture, the killing that developed was weak and antigenically nonspecific. Transfer of cultured, tumourdraining LNC to immunologically compromised, syngeneic mice conferred complete protection from Moloney sarcoma progression. Adoptive transfer could be delayed for 6 days after tumour induction without loss of protection. These results suggest that there exists in Moloney sarcoma-bearing mice a mechanism that limits the differentiation of pre-killer cells into cytolytically active T lymphocytes, and that such inhibition is eliminated when LNC are explanted into culture.

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The cellular events associated with regression and progression of murine (moloney) sarcomas

Cited by 32 publications

References 10 publications

Relationship of tumor leucocytic infiltration to host defense mechanisms and prognosis

Relationship of tumor leucocytic infiltration to host defense mechanisms and prognosis

Development and persistence of cytolytic T lymphocytes in regressing or progressing moloney sarcomas

Resurgence of killing and in vivo protection mediated by lymphocytes cultured from lymph nodes draining Moloney sarcomas

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