The cellular protein expression of Foxp3 in lymphoid and non-lymphoid organs of Nile tilapia

Jia, Cunxin; Zhou, Yujie; Huang, Xiaohuan; Peng, Xi; Linyan, Liu; Zhou, Lei; Jin, Li; Shi, Hongjuan; Wei, Jing; Wang, Deshou

doi:10.1016/j.fsi.2015.03.021

Cited by 11 publications

(5 citation statements)

References 29 publications

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“…Among gut advantage ones, many MHC (mainly II) and immunoglobulin (IgM and the other, maybe IgT) transcripts might imply existing of fish MHC II-expressed intestinal innate lymphocytes (ILCs), since that such population may limit expansion of pathological CD4 + T cell and therefore induce IgA production in mammalian intestine32. And many immunoregulatory factors were also found in gut advantage transcripts, such as SOCSs (suppressor of cytokine signaling proteins, may inhibit the activity of JAKs and STATs33) in region a, LITRs (Leukocyte immune-type receptors, might inhibit cellular cytotoxicity34) in region d, IL-1R (may neutralize IL-1) in region d, as well as Foxp3 in region a and CD73 in region d (might be the key transcription factor3536 and effecter373839 of Treg respectively). Also intestinal c-type lectins in regions a, d and e suggested their positive relation to maintain immune homeostasis40, which was coincident with that mammalian CLRs (C-type lectin receptors) on macrophages or dendritic cells (DCs) are involved in IL-10 production and Treg maturation641.…”

Section: Discussionmentioning

confidence: 99%

Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies

Song

Wang

et al. 2016

Sci Rep

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The gut-associated lymphoid tissue, connected with liver via bile and blood, constructs a local immune environment of both defense and tolerance. The gut-liver immunity has been well-studied in mammals, yet in fish remains largely unknown, even though enteritis as well as liver and gallbladder syndrome emerged as a limitation in aquaculture. In this study, we performed integrative bioinformatic analysis for both transcriptomic (gut and liver) and proteomic (intestinal mucus and bile) data, in both healthy and infected tilapias. We found more categories of immune transcripts in gut than liver, as well as more adaptive immune in gut meanwhile more innate in liver. Interestingly reduced differential immune transcripts between gut and liver upon inflammation were also revealed. In addition, more immune proteins in bile than intestinal mucus were identified. And bile probably providing immune effectors to intestinal mucus upon inflammation was deduced. Specifically, many key immune transcripts in gut or liver as well as key immune proteins in mucus or bile were demonstrated. Accordingly, we proposed a hypothesized profile of fish gut-liver immunity, during either homeostasis or inflammation. Current data suggested that fish gut and liver may collaborate immunologically while keep homeostasis using own strategies, including potential unique mechanisms.

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Section: Discussionmentioning

confidence: 99%

Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies

Song

Wang

et al. 2016

Sci Rep

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“…Accordingly, the expression patterns of Foxp3a and IL-17C1 in our study, given their functional capabilities, may demonstrate a counterbalance attempt to maintain tolerance, given the immune landscape. However, there may also be much broader implications for Foxp3, as demonstrated by a recent Nile tilapia study, which found Foxp3 protein expression present even in non-hematopoietic tissues in non-lymphoid organs [45].…”

Section: At 15 °C the Host Immune Responsementioning

confidence: 99%

Who needs the hotspot? The effect of temperature on the fish host immune response to Tetracapsuloides bryosalmonae the causative agent of proliferative kidney disease

Bailey

Segner

Casanova-Nakayama

et al. 2017

Fish & Shellfish Immunology

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Proliferative kidney disease (PKD) of salmonids, caused by Tetracapsuloides bryosalmonae may lead to high mortalities at elevated water temperatures. However, it has not yet been investigated how temperature affects the fish host immune response to T. bryosalmonae. We exposed YOY (young of the year) rainbow trout (Oncorhynchus mykiss) to T. bryosalmonae at two temperatures (12 °C and 15 °C) that reflect a realistic environmental scenario and could occur in the natural habitat of salmonids. We followed the development of the parasite, host pathology and immune response over seven weeks. We evaluated the composition and kinetics of the leukocytes and their major subgroups in the anterior and posterior kidney. We measured immune gene expression profiles associated with cell lineages and functional pathways in the anterior and posterior kidney. At 12 °C, both infection prevalence and pathogen load were markedly lower. While the immune response was characterized by subtle changes, mainly an increased amount of lymphocytes present in the kidney, elevated expression of Th1-like signature cytokines and strong upregulation of the natural killer cell enhancement factor, NKEF at week 6 P.E. At 15 °C the infection prevalence and pathogen burden were ominously greater. While the immune response as the disease progressed was associated with a Th2-like switch at week 6 P.E and a prominent B cell response, evidenced at the tissue, cell and transcript level. Our results highlight how a subtle, environmentally relevant difference in temperature resulted in diverse outcomes in terms of the immune response strategy, altering the type of interaction between a host and a parasite.

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“…More importantly, T-bet was found to be expressed in the nucleus of lymphocytes in flounder. Forkhead box protein 3 (Foxp3), a crucial transcription regulator in the generation and function of CD4 + CD25 + regulatory T cells (Treg), was also reported to be localized in the nucleus of peripheral blood mononuclear cells in tilapia ( Oreochromis niloticus ) ( 36 ). Similarly, human T-bet-specific mAb 4B10 could recognize an approximately 62 kDa protein present nuclear extracts prepared from Th1 cell clones, but not Th2 clones ( 13 ).…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of a Master Transcription Factor of Th1 Cells, T-bet, Within Flounder (Paralichthys olivaceus)

et al. 2021

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T-bet, a T-box family member, is a transcription factor essential for the differentiation of naive CD4+ T cells into Th1 cells that are involved in both innate and adaptive immune responses. In this study, the transcription factor T-bet of flounder (Paralichthys olivaceus) was cloned and characterized, and its expression profile after infection was analyzed. T-bet+ cells were identified in flounder, and the expression and localization of T-bet in T lymphocyte subsets and B lymphocytes were investigated. Finally, the proliferation of T-bet+ cells, T lymphocyte subsets, and B lymphocytes were studied after stimulation with IFN-γ, IL-2, and IL-6, respectively, and the variations of some transcription factors and cytokines in CD4+ T lymphocyte subsets were detected. The results showed that T-bet in flounder consists of 619 aa with a conserved T-box DNA binding domain. T-bet was abundantly expressed in the spleen, head kidney, and heart, and it was significantly upregulated after infection with Vibrio anguillarum, Edwardsiella tarda, and Hirame rhabdovirus, especially in the group of Edwardsiella tarda. A polyclonal antibody against recombinant protein of T-bet was prepared, which specifically recognized the natural T-bet molecule in flounder. T-bet+ cells were found to be distributed in the lymphocytes of peripheral blood, spleen, and head kidney, with the highest proportion in spleen, and the positive signals of T-bet occurred in the cell nucleus. T-bet was also detected in the sorted CD4-1+, CD4-2+, CD8+ T lymphocytes, and IgM+ B lymphocytes. In addition, T-bet+ cells, coordinated with CD4-1+ and CD4-2+ T lymphocytes, were proliferated after stimulation with IFN-γ, IL-2, and IL-6. Especially in sorted CD4-1+ and CD4-2+ T lymphocytes, IFN-γ and IL-2 were able to upregulate the expression of T-bet, forming a positive feedback loop in Th1-type cytokine secretion. These results suggest that T-bet may act as a master transcription factor regulating flounder CD4+ T lymphocytes involved in a Th1-type immune response.

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The cellular protein expression of Foxp3 in lymphoid and non-lymphoid organs of Nile tilapia

Cited by 11 publications

References 29 publications

Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies

Fish gut-liver immunity during homeostasis or inflammation revealed by integrative transcriptome and proteome studies

Who needs the hotspot? The effect of temperature on the fish host immune response to Tetracapsuloides bryosalmonae the causative agent of proliferative kidney disease

Identification and Characterization of a Master Transcription Factor of Th1 Cells, T-bet, Within Flounder (Paralichthys olivaceus)

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