The cellular responses of autophagy, apoptosis, and 5-methylcytosine level in zebrafish cells upon nutrient deprivation stress

Fan, Xiaoteng; Hou, Tingting; Zhang, Shuai; Guan, Yongjing; Jia, Jia; Wang, Zaizhao

doi:10.1016/j.chemosphere.2019.124989

Cited by 20 publications

(5 citation statements)

References 71 publications

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“…In our study, we observed that the expression of lamp1 in the liver was significantly induced after starvation, and then returned to normal levels after refeeding (Figure 8A). This finding was consistent with previous studies in zebrafish (Fan et al, 2020), suggesting that the lamp1 gene may be involved in energy regulation via autophagy in largemouth bass. To validate that autophagy occurred after long-term starvation, the transcription level of two classical autophagosomal markers, Beclin1 and MAPLC3 (Yabu et al, 2012;Al-Shenawy, 2016), was detected in three tissues (liver, spleen and kidney) of largemouth bass in the present study.…”

Section: Discussionsupporting

confidence: 93%

“…Other studies have shown that nutrient restriction greatly increased the expression of Atg genes (atg4, atg9, atg12, lc3, gabarap and becn1) in gill epithelial cells of O. mykiss (Walbaum) (Balmori-Cedeño et al, 2019). A recent study reported that starvation stimulates lamp1 expression in zebrafish embryonic fibroblast cells (Fan et al, 2020). However, further research and exploration are needed to study the lamp family genes under food-restricted conditions in teleosts.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of three lamp genes from largemouth bass (Micropterus salmoides): molecular cloning, expression patterns, and their transcriptional levels in response to fast and refeeding strategy

Yang,

Zeng,

Peng

et al. 2024

Front. Physiol.

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Lysosomes-associated membrane proteins (LAMPs), a family of glycosylated proteins and major constituents of the lysosomal membranes, play a dominant role in various cellular processes, including phagocytosis, autophagy and immunity in mammals. However, their roles in aquatic species remain poorly known. In the present study, three lamp genes were cloned and characterized from Micropterus salmoides. Subsequently, their transcriptional levels in response to different nutritional status were investigated. The full-length coding sequences of lamp1, lamp2 and lamp3 were 1251bp, 1224bp and 771bp, encoding 416, 407 and 256 amino acids, respectively. Multiple sequence alignment showed that LAMP1-3 were highly conserved among the different fish species, respectively. 3-D structure prediction, genomic survey, and phylogenetic analysis were further confirmed that these genes are widely existed in vertebrates. The mRNA expression of the three genes was ubiquitously expressed in all selected tissues, including liver, brain, gill, heart, muscle, spleen, kidney, stomach, adipose and intestine, lamp1 shows highly transcript levels in brain and muscle, lamp2 displays highly expression level in heart, muscle and spleen, but lamp3 shows highly transcript level in spleen, liver and kidney. To analyze the function of the three genes under starvation stress in largemouth bass, three experimental treatment groups (fasted group and refeeding group, control group) were established in the current study. The results indicated that the expression of lamp1 was significant induced after starvation, and then returned to normal levels after refeeding in the liver. The expression of lamp2 and lamp3 exhibited the same trend in the liver. In addition, in the spleen and the kidney, the transcript level of lamp1 and lamp2 was remarkably increased in the fasted treatment group and slightly decreased in the refed treatment group, respectively. Collectively, our findings suggest that three lamp genes may have differential function in the immune and energetic organism in largemouth bass, which is helpful in understanding roles of lamps in aquatic species.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Characterization of three lamp genes from largemouth bass (Micropterus salmoides): molecular cloning, expression patterns, and their transcriptional levels in response to fast and refeeding strategy

Yang,

Zeng,

Peng

et al. 2024

Front. Physiol.

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“…Starvation and H 2 O 2 activate macroautophagy [29][30][31] . To determine whether the observed puncta could be attributed to a possible macroautophagy activity, we next used antisense morpholino oligonucleotides (Mo) to inhibit translation of zebrafish mRNAs encoding the E1like enzyme Atg7, a core macroautophagy-related factor involved in the conjugation of Atg5 to Atg12 32 .…”

Section: Zebrafish Cells Recapitulate a Whole Cma Process From Kferq-...mentioning

confidence: 99%

The zebrafish as a new model for studying chaperone-mediated autophagy unveils its role in spermatogenesis

Goguet,

Vélez,

Schnebert

et al. 2024

Preprint

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Chaperone-Mediated Autophagy (CMA) is a major pathway of lysosomal proteolysis involved in numerous cellular processes, and whose dysfunction is associated to several pathologies. Initially studied in mammals and birds, recent findings have identified CMA in fish, reshaping our understanding of its evolution across metazoans. Given the exciting perspectives this finding offered, we have now developed the required tools to investigate and functionally asses that CMA function in a powerful fish genetic model: the zebrafish (Danio rerio). After adapting and validating a fluorescent reporter (KFERQ-Dendra2; previously used to track CMA in mammalian cells) in zebrafish primary embryonic cells, we first demonstrated CMA functionality in this fish species. Then, we developed a transgenic zebrafish line expressing the KFERQ-Dendra2 CMA reporter, enabling the real-time tracking of CMA activityin vivo. This model revealed heterogeneous CMA responses within tissues, highlighting the zebrafish as a valuable model for investigating tissue-specific and cell-scale variations in CMA. Moreover, a novel role for CMA has been uncovered, acting as a gatekeeper of sperm cell proteostasis, thereby playing a crucial role in the production of active and high-quality spermatozoa. Overall, these findings emphasize the zebrafish as a pivotal model for advancing our comprehension of the fundamental mechanisms underlying CMA.

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“…The mitochondria-lysosomal contact sites through autophagosomes play a crucial role in the autophagy, biogenesis of both the organelles and exchanges of lipid, amino acids etc. Autophagy is essential for neuronal survival and serves as an adaptive response to nutrient deprivation (Clarke and Mearow, 2016; Fan et al, 2020). Lysosomes play an important role in clearing the damaged cellular components through autophagy (Li et al, 2019; Bi et al, 2021).…”

Section: Lysosomal Dysfunction In Pdmentioning

confidence: 99%

Mitochondrial and Organellar Crosstalk in Parkinson’s Disease

et al. 2021

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Mitochondrial dysfunction is a well-established pathological event in Parkinson’s disease (PD). Proteins misfolding and its impaired cellular clearance due to altered autophagy/mitophagy/pexophagy contribute to PD progression. It has been shown that mitochondria have contact sites with endoplasmic reticulum (ER), peroxisomes and lysosomes that are involved in regulating various physiological processes. In pathological conditions, the crosstalk at the contact sites initiates alterations in intracellular vesicular transport, calcium homeostasis and causes activation of proteases, protein misfolding and impairment of autophagy. Apart from the well-reported molecular changes like mitochondrial dysfunction, impaired autophagy/mitophagy and oxidative stress in PD, here we have summarized the recent scientific reports to provide the mechanistic insights on the altered communications between ER, peroxisomes, and lysosomes at mitochondrial contact sites. Furthermore, the manuscript elaborates on the contributions of mitochondrial contact sites and organelles dysfunction to the pathogenesis of PD and suggests potential therapeutic targets.

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The cellular responses of autophagy, apoptosis, and 5-methylcytosine level in zebrafish cells upon nutrient deprivation stress

Cited by 20 publications

References 71 publications

Characterization of three lamp genes from largemouth bass (Micropterus salmoides): molecular cloning, expression patterns, and their transcriptional levels in response to fast and refeeding strategy

Characterization of three lamp genes from largemouth bass (Micropterus salmoides): molecular cloning, expression patterns, and their transcriptional levels in response to fast and refeeding strategy

The zebrafish as a new model for studying chaperone-mediated autophagy unveils its role in spermatogenesis

Mitochondrial and Organellar Crosstalk in Parkinson’s Disease

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