1984
DOI: 10.1099/00221287-130-6-1367
|View full text |Cite
|
Sign up to set email alerts
|

The Cellulase System of Cellulomonas fimi

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

1
31
0
2

Year Published

1986
1986
2018
2018

Publication Types

Select...
9

Relationship

0
9

Authors

Journals

citations
Cited by 39 publications
(34 citation statements)
references
References 21 publications
1
31
0
2
Order By: Relevance
“…The possibility that EG3 is a lipoprotein has already been discussed, and this could account for the difference in molecular mass between the 73-kDa protein coded for by the ORF of cel-3 and the 94-kDa membrane-associated form observed in E. coli. In addition, synthesis of glycosylated endoglucanases is a common feature of cellulolytic bacteria (2,6,31,41). Hence, the possibility that EG3 is a glycoprotein in F. succinogenes must also be considered.…”
Section: Resultsmentioning
confidence: 99%
“…The possibility that EG3 is a lipoprotein has already been discussed, and this could account for the difference in molecular mass between the 73-kDa protein coded for by the ORF of cel-3 and the 94-kDa membrane-associated form observed in E. coli. In addition, synthesis of glycosylated endoglucanases is a common feature of cellulolytic bacteria (2,6,31,41). Hence, the possibility that EG3 is a glycoprotein in F. succinogenes must also be considered.…”
Section: Resultsmentioning
confidence: 99%
“…Also, in Ruminococcus albus, most of the cellulase activity was found to be cell bound (58). In other gram-positive bacteria, cellulases are present in the culture supernatant, e.g., in C. fimi (33), Streptomyces lividans (54), and Franckia sp. (49); many gram-negative bacteria also release cellulases, e.g., Fibrobacter sluccinogenes (40) and Prevotella rluminicola (39).…”
Section: Methodsmentioning
confidence: 99%
“…Cultures were harvested when the initial 02 concentration of 1.7% in the headspace had decreased to 0.8%; atmospheric 02 was measured with a GC1 1 gas chromatograph with a thermal conductivity detector (Delsi Instruments, Suresnes, France) by using a Molecular Sieve DS column (2 m, 1/8-mm DS, 80 to 100 mesh; Alltech). To measure the expression of cellulase by Azoarcus spp., a modification of a medium proposed by Kim and Wimpenny (KW medium [29]) was used, which contained 0.4% (NH4)2S04, 0.01% NaCl, 0.01% MgSO4, 0.01% CaCl2, 0.05% yeast extract, 33 (47).…”
Section: Methodsmentioning
confidence: 99%
“…When grown in the presence of cellulose, Cellulomonas fimi produces a variety of polypeptides with carboxymethylcellulase activity, some of which may arise by deglycosylation or proteolysis of native enzymes (25). This complicates the purification of the native polypeptides.…”
mentioning
confidence: 99%