The Cellulosome: A Fiber-Degrading Strategist of the Rumen Microbiome

Mukkala, Srijana; Bramhachari, Pallaval Veera; Reddy, Y. Harish Kumar

doi:10.1007/978-981-19-3696-8_11

Cited by 5 publications

(3 citation statements)

References 126 publications

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“…Conversely, anaerobic cellulolytic microorganisms secrete cellulases as cellulosomes, wherein enzymes are associated with accessory proteins that directly bind to cellulose, rather than being bound to a cellulosebinding module [55]. Cellulosomes were first identified in the early 1980s through research conducted on the thermophilic anaerobic bacterium called Clostridium thermocellum, and subsequent research has revealed other cellulosome-producing anaerobic microorganisms from diverse environments [56,57]. However, due to the technical challenges associated with extracting cellulases from cellulosomes, aerobic microorganisms are generally preferred for industrial cellulase production [52].…”

Section: Cellulasesmentioning

confidence: 99%

The Role of Microbial Diversity in Lignocellulosic Biomass Degradation: A Biotechnological Perspective

Rasool,

Irfan

2024

ChemBioEng Reviews

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Lignocellulosic biomass, such as plant residues and agricultural waste, holds immense potential as a renewable resource for the production of biofuels, chemicals, and animal feed. However, the efficient degradation of lignocellulose into fermentable sugars remains a significant challenge. Recent research has highlighted the critical role of microbial diversity in lignocellulosic biomass degradation, offering new insights from a biotechnological perspective. The comprehension and utilization of microbial diversity are crucial for developing efficient biotechnological strategies for lignocellulosic biomass degradation. By uncovering the intricate relationships between microbial communities and their enzymatic machinery, researchers can optimize degradation processes, enhance biofuel production, and contribute to a more sustainable bio‐based economy. Microorganisms, including bacteria, fungi, and archaea, possess diverse enzymatic capabilities, allowing them to secrete a plethora of lignocellulolytic enzymes. Microbial organisms inhabiting extreme environments, such as the rumen, hot and cold springs, deep sea trenches, and acidic and alkaline pH environments, exhibit significant potential in generating enzymes, including hemicellulolytic and lignocellulolytic enzymes, which possess superior biochemical properties essential for industrial bioconversion applications. This review explores the ability of lignocellulosic enzymes from microbial sources to efficiently break down the lignocellulosic biomass and their potential applications in industrial biotechnology.

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Section: Cellulasesmentioning

confidence: 99%

The Role of Microbial Diversity in Lignocellulosic Biomass Degradation: A Biotechnological Perspective

Rasool,

Irfan

2024

ChemBioEng Reviews

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“…The cellulosome complex was first discovered from the thermophilic anaerobic bacteria Clostridium thermocellum in the early 1980s. Since then, many cellulosome-producing anaerobic microorganisms have been identified and isolated from different ecosystems [ 81 , 82 , 83 , 84 , 85 , 86 ]. However, since the cellulosome complex makes it challenging to extract cellulases, aerobic microorganisms are usually preferred over anaerobic microorganisms for the industrial production of cellulases [ 76 ].…”

Section: Lignocellulolytic Enzymesmentioning

confidence: 99%

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Benatti

Polizeli

2023

Microorganisms

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Human population growth, industrialization, and globalization have caused several pressures on the planet’s natural resources, culminating in the severe climate and environmental crisis which we are facing. Aiming to remedy and mitigate the impact of human activities on the environment, the use of lignocellulolytic enzymes for biofuel production, food, bioremediation, and other various industries, is presented as a more sustainable alternative. These enzymes are characterized as a group of enzymes capable of breaking down lignocellulosic biomass into its different monomer units, making it accessible for bioconversion into various products and applications in the most diverse industries. Among all the organisms that produce lignocellulolytic enzymes, microorganisms are seen as the primary sources for obtaining them. Therefore, this review proposes to discuss the fundamental aspects of the enzymes forming lignocellulolytic systems and the main microorganisms used to obtain them. In addition, different possible industrial applications for these enzymes will be discussed, as well as information about their production modes and considerations about recent advances and future perspectives in research in pursuit of expanding lignocellulolytic enzyme uses at an industrial scale.

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“…By producing a variety of carbohydrate-active enzymes such as endo-1,4-glucanases, xylanases, and cellulases, Ruminococcus spp. can break down plant cell walls, which can decrease fiber and increase crude protein [ 8 ]. To increase feed efficiency and performance in poultry, an alternate strategy is the use of rumen bacterial inoculum as a feed additive.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

Alam,

Hossain,

Sohidullah

et al. 2024

J Adv Vet Anim Res

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Objective: To isolate, and characterize cellulolytic rumen bacteria from the rumen of Sahiwal cattle utilizing rumen bacterial inoculum to increase the nutritional value of rice bran used as broiler feed. Materials and Methods: The ruminal liquid was kept at an optimal pH of 6.9, and a redox potential of less than -300mV while being incubated anaerobically at 39℃ in a medium containing Rumen Fluid Glucose Cellobiose Agar. By using the Hungate technique, the isolates were detected on the basis of their morphological, physiological, biochemical, and molecular testing. Results: The findings revealed that the isolated Ruminococcus albus, and Ruminococcus flavifaciens were obligate anaerobic, generally Gram-positive, non-motile cocci or rod, single or pair, occasionally short chain, producing yellow pigment when grown on cellulose, and presence of clear zone around the colonies. Both isolates fermented sugars such cellobiose, glucose, and lactose as well as decomposed xylan. The results also showed that the isolates were recognized as Ruminococcus spp., a cellulolytic rumen bacterium, were catalase negative, indole negative, and gelatin liquefaction positive. Conclusion: Isolation and characterization of Ruminococcus spp. may be helpful for Bangladesh in reducing the cost of producing poultry feed, and circumventing restrictions on rice bran use. We can also develop more efficient and long-lasting plans to enhance poultry performance, and feed efficiency as well as increase the nutritional value of rice bran used as broiler feed through understanding how various Ruminococcus spp. function in this process.

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The Cellulosome: A Fiber-Degrading Strategist of the Rumen Microbiome

Cited by 5 publications

References 126 publications

The Role of Microbial Diversity in Lignocellulosic Biomass Degradation: A Biotechnological Perspective

The Role of Microbial Diversity in Lignocellulosic Biomass Degradation: A Biotechnological Perspective

Lignocellulolytic Biocatalysts: The Main Players Involved in Multiple Biotechnological Processes for Biomass Valorization

Isolation and characterization of strictly anaerobic cellulolytic rumen bacterial species from Sahiwal cattle

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