The Central Cannabinoid Receptor Inactivation Suppresses Endocrine Reproductive Functions

Wenger, T.; Ledent, Catherine; Csernus, Valér; Gerendai, Ida

doi:10.1006/bbrc.2001.4977

Cited by 146 publications

(143 citation statements)

References 23 publications

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“…Some other studies also showed that testosterone levels did not vary much after marijuana use [83,86]. These observations are interesting in spite of the fact that CB1 receptor activation by AEA caused a drop in testosterone levels [56] and that animal models (rats and monkeys) showed a marked reduction in testosterone in response to THC and CBD treatment [93,94].…”

Section: Effect On the Hpg Axismentioning

confidence: 83%

“…CB2 was found in Sertoli cells while other components of the ECS, such as AEA, FAAH, and EMT have also been observed in testicular tissues [4,19,49,56]. For example, administration of AEA, which usually binds to postsynaptic CB1 receptors, decreased serum LH.…”

Section: Ecs and The Hypothalamus-pituitary-gonadal Axismentioning

confidence: 98%

“…LH and testosterone secretion were decreased in CB1 receptor-inactivated mice. However, in wild-type mice, AEA suppressed the levels of both of these hormones [56]. When they are activated through the endogenous cannabinoid AEA, it not only results in a drop in testosterone levels, but this alteration in sex steroid level can also disturb the spermatogenic process [19,45,56].…”

Section: Ecs and The Hypothalamus-pituitary-gonadal Axismentioning

confidence: 99%

“…For example, administration of AEA, which usually binds to postsynaptic CB1 receptors, decreased serum LH. This action could be prevented by a specific CB1 antagonist (SR141716) [56,57]. Farkas and coworkers furthermore demonstrated that endocannabinoid activates CB1 which, in turn, inhibits spontaneous gamma aminobutyric acid (GABA) release [58].…”

Section: Ecs and The Hypothalamus-pituitary-gonadal Axismentioning

confidence: 99%

“…However, in wild-type mice, AEA suppressed the levels of both of these hormones [56]. When they are activated through the endogenous cannabinoid AEA, it not only results in a drop in testosterone levels, but this alteration in sex steroid level can also disturb the spermatogenic process [19,45,56]. It was furthermore showed that these CB1 receptors are responsible for the actions of exogenous cannabinoids [56].…”

Section: Ecs and The Hypothalamus-pituitary-gonadal Axismentioning

confidence: 99%

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Marijuana, phytocannabinoids, the endocannabinoid system, and male fertility

Plessis

Agarwal

Syriac

2015

J Assist Reprod Genet

126

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Marijuana has the highest consumption rate among all of the illicit drugs used in the USA, and its popularity as both a recreational and medicinal drug is increasing especially among men of reproductive age. Male factor infertility is on the increase, and the exposure to the cannabinoid compounds released by marijuana could be a contributing cause. The endocannabinoid system (ECS) is deeply involved in the complex regulation of male reproduction through the endogenous release of endocannabinoids and binding to cannabinoid receptors. Disturbing the delicate balance of the ECS due to marijuana use can negatively impact reproductive potential. Various in vivo and in vitro studies have reported on the empirical role that marijuana plays in disrupting the hypothalamus-pituitary-gonadal axis, spermatogenesis, and sperm function such as motility, capacitation, and the acrosome reaction. In this review, we highlight the latest evidence regarding the effect of marijuana use on male fertility and also provide a detailed insight into the ECS and its significance in the male reproductive system.

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Section: Effect On the Hpg Axismentioning

confidence: 83%

Section: Ecs and The Hypothalamus-pituitary-gonadal Axismentioning

confidence: 98%

Section: Ecs and The Hypothalamus-pituitary-gonadal Axismentioning

confidence: 99%

Section: Ecs and The Hypothalamus-pituitary-gonadal Axismentioning

confidence: 99%

Section: Ecs and The Hypothalamus-pituitary-gonadal Axismentioning

confidence: 99%

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Marijuana, phytocannabinoids, the endocannabinoid system, and male fertility

Plessis

Agarwal

Syriac

2015

J Assist Reprod Genet

126

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Human Sperm Anatomy: Ultrastructural Localization of the Cannabinoid1 Receptor and a Potential Role of Anandamide in Sperm Survival and Acrosome Reaction

Aquila

Guido

Santoro

et al. 2010

The Anatomical Record

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Recently, the endocannabinoid (EC) system and the presence of CB1 receptor (CB1-R), have been identified in human sperm. However, the effects of EC receptor ligands such as anandamide (N-arachidonoylethanolamine) and the role of EC system in male fertility is still largely unexplored. In the present study, we investigated the ultrastructural compartmentalization of CB1-R and analyzed the effects of its stimulation by using a stable analog of anandamide, 2-methylarachidonyl-2 0 -fluoro-ethylamide (MET-F-AEA). We focused particularly on sperm survival and acrosin activity. The study of human sperm anatomy by transmission electron microscopy with immunogold analysis revealed the location of the CB1-R prevalently in the sperm membranes of the head and interestingly on the mitochondria. The effect of different concentrations of MET-F-AEA from 100 nM to 1 lM evidenced a significant decrease of sperm survival. Interestingly, we analyzed this negative effect at molecular level, testing the EC action on different known sperm survival targets. MET-F-AEA-treatment decreased both pBCL2 and pAkt, two prosurvival proteins, and increased pPTEN expression which is the main regulator of the PI3K/Akt pathway. Moreover, a biphasic effect was observed with increasing MET-F-AEA concentrations on the acrosin activity. The blockage of the CB1-R by using its selective antagonist SR141716 (rimonabant) induced an opposite action on sperm survival supporting a role for this receptor in the biology of the male gamete. Anat Rec,[293][294][295][296][297][298][299][300][301][302][303][304][305][306][307][308][309]

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