The centriolar satellite protein SSX2IP promotes centrosome maturation

Bärenz, Felix; Inoue, Daigo; Yokoyama, Hideki; Tegha-Dunghu, Justus; Freiss, Stephanie; Draeger, Stefanie; Mayilo, Dmytro; Cado, Ivana; Merker, Sabine; Klinger, Maren; Hoeckendorf, Burkhard; Pilz, Sahra; Hupfeld, Kerstin; Steinbeißer, Herbert; Lorenz, Holger; Ruppert, Thomas; Wittbrodt, Joachim; Gruß, Oliver J.

doi:10.1083/jcb.201302122

Cited by 61 publications

(97 citation statements)

References 58 publications

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“…Despite its prevailed phenomenon and biological significance, the molecular mechanism underlying microtubule anchoring had mostly been enigmatic until recently [142]. However, since the conserved protein family collectively called the MSD1/SSX2IP family consisting of fission yeast Msd1, filamentous fungus ( Aspergillus nidulans ) TINA, chicken LCG, murine ADIP and fish/frog/human SSX2IP [22, 24, 88, 143–147] was shown to comprise critical microtubule-anchoring factors, the underlying mechanism has begun to be revealed. The role for MSD1/SSX2IP in microtubule anchoring was first identified in fission yeast, in which Msd1 is required for tethering the minus ends of mitotic spindle microtubules to the SPB [143].…”

Section: Box: Anchoring Of the Microtubule Minus End To The Centrosommentioning

confidence: 99%

Regulation of centriolar satellite integrity and its physiology

Hori

Toda

2016

Cell. Mol. Life Sci.

108

140

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Centriolar satellites comprise cytoplasmic granules that are located around the centrosome. Their molecular identification was first reported more than a quarter of a century ago. These particles are not static in the cell but instead constantly move around the centrosome. Over the last decade, significant advances in their molecular compositions and biological functions have been achieved due to comprehensive proteomics and genomics, super-resolution microscopy analyses and elegant genetic manipulations. Centriolar satellites play pivotal roles in centrosome assembly and primary cilium formation through the delivery of centriolar/centrosomal components from the cytoplasm to the centrosome. Their importance is further underscored by the fact that mutations in genes encoding satellite components and regulators lead to various human disorders such as ciliopathies. Moreover, the most recent findings highlight dynamic structural remodelling in response to internal and external cues and unexpected positive feedback control that is exerted from the centrosome for centriolar satellite integrity.

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Section: Box: Anchoring Of the Microtubule Minus End To The Centrosommentioning

confidence: 99%

Regulation of centriolar satellite integrity and its physiology

Hori

Toda

2016

Cell. Mol. Life Sci.

108

140

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“…The immunoprecipitates were separated by SDS-PAGE for immunoblotting. We also analysed control and MEL-28 immunoprecipitates by mass spectrometry 32 . In brief, proteins of the immunoprecipitates were separated by SDS-PAGE, and proteins in-gel were digested by trypsin.…”

Section: Methodsmentioning

confidence: 99%

The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation

et al. 2014

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The GTP-bound form of the Ran GTPase (RanGTP), produced around chromosomes, drives nuclear envelope and nuclear pore complex (NPC) re-assembly after mitosis. The nucleoporin MEL-28/ELYS binds chromatin in a RanGTP-regulated manner and acts to seed NPC assembly. Here we show that, upon mitotic NPC disassembly, MEL-28 dissociates from chromatin and re-localizes to spindle microtubules and kinetochores. MEL-28 directly binds microtubules in a RanGTP-regulated way via its C-terminal chromatin-binding domain. Using Xenopus egg extracts, we demonstrate that MEL-28 is essential for RanGTP-dependent microtubule nucleation and spindle assembly, independent of its function in NPC assembly. Specifically, MEL-28 interacts with the g-tubulin ring complex and recruits it to microtubule nucleation sites. Our data identify MEL-28 as a RanGTP target that functions throughout the cell cycle. Its cell cycle-dependent binding to chromatin or microtubules discriminates MEL-28 functions in interphase and mitosis, and ensures that spindle assembly occurs only after NPC breakdown.

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“…Cells were blocked by 10% FCS and 0.1% triton in PBS for 1 h at RT. Cells were incubated with primary antibodies against PCM1 (Cell Signaling), γ-tubulin (Bärenz et al 2013), GM130 (MBL), and GOPC (Sigma-Aldrich) diluted in 10% FCS in PBS overnight at 4°C. Alexa Fluor 594 (Life Technologies) secondary antibodies were incubated with the cells for 2 h at RT.…”

Section: Microscopymentioning

confidence: 99%

NSUN6 is a human RNA methyltransferase that catalyzes formation of m⁵C72 in specific tRNAs

Haag

Warda

Kretschmer

et al. 2015

RNA

166

151

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Many cellular RNAs require modification of specific residues for their biogenesis, structure, and function. 5-methylcytosine (m 5 C) is a common chemical modification in DNA and RNA but in contrast to the DNA modifying enzymes, only little is known about the methyltransferases that establish m 5 C modifications in RNA. The putative RNA methyltransferase NSUN6 belongs to the family of Nol1/Nop2/SUN domain (NSUN) proteins, but so far its cellular function has remained unknown. To reveal the target spectrum of human NSUN6, we applied UV crosslinking and analysis of cDNA (CRAC) as well as chemical crosslinking with 5-azacytidine. We found that human NSUN6 is associated with tRNAs and acts as a tRNA methyltransferase. Furthermore, we uncovered tRNA Cys and tRNA Thr as RNA substrates of NSUN6 and identified the cytosine C72 at the 3 ′ end of the tRNA acceptor stem as the target nucleoside. Interestingly, target recognition in vitro depends on the presence of the 3 ′ -CCA tail. Together with the finding that NSUN6 localizes to the cytoplasm and largely colocalizes with marker proteins for the Golgi apparatus and pericentriolar matrix, our data suggest that NSUN6 modifies tRNAs in a late step in their biogenesis.

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The centriolar satellite protein SSX2IP promotes centrosome maturation

Abstract: SSX2IP promotes centrosome maturation and maintenance at the onset of vertebrate development, preserving centrosome integrity and mitosis during rapid cleavage divisions and in somatic cells.

Cited by 61 publications

References 58 publications

Regulation of centriolar satellite integrity and its physiology

Regulation of centriolar satellite integrity and its physiology

The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation

NSUN6 is a human RNA methyltransferase that catalyzes formation of m⁵C72 in specific tRNAs

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The centriolar satellite protein SSX2IP promotes centrosome maturation

Abstract: SSX2IP promotes centrosome maturation and maintenance at the onset of vertebrate development, preserving centrosome integrity and mitosis during rapid cleavage divisions and in somatic cells.

Cited by 61 publications

References 58 publications

Regulation of centriolar satellite integrity and its physiology

Regulation of centriolar satellite integrity and its physiology

The nucleoporin MEL-28 promotes RanGTP-dependent γ-tubulin recruitment and microtubule nucleation in mitotic spindle formation

NSUN6 is a human RNA methyltransferase that catalyzes formation of m5C72 in specific tRNAs

Contact Info

Product

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About

NSUN6 is a human RNA methyltransferase that catalyzes formation of m⁵C72 in specific tRNAs