The centromere‐binding factor Cbf1p from Candida albicans complements the methionine auxotrophic phenotype of Saccharomyces cerevisiae

Abstract

A gene encoding the centromere binding factor 1 (Cbf1p) of the human pathogenic yeast Candida albicans was cloned and characterized. An open reading-frame was detected which encoded a 223 amino acid protein with a calculated molecular weight of 25.8 kDa and a relative isoelectric point of 5.55. It shares 39% overall amino acid sequence identity with Saccharomyces cerevisiae Cbf1p. We localized the CaCBF1 gene on chromosome 4. Southern analysis indicated that CaCBF1 is probably present as a single copy gene per haploid genome. The CaCBF1 gene under the control of its own promoter was able to complement the methionine auxotrophic growth, the increased mitotic instability of CEN plasmids and the slow growth of a Saccharomyces cerevisiae cbf1D mutant strain. The

l-Methionine repressible promoters for tuneable gene expression in Trichoderma reesei

“…2 ). Interestingly, 7 of the 10 l -methionine biosynthesis genes of T. reesei also lacked a CACGTG consensus sequence that is bound in S. cerevisiae by the Cbf1 protein and involved in the regulation of MET genes [ 23 , 24 ]. When balancing the genes repressed by the addition of 0.1 mM l -methionine to a wheat straw culture, it is intriguing to note that more than a third (13 of 37) of them encode proteins either involved in iron acquisition (iron transporter, ferrooxidoreductase, siderophore biosynthesis, oxalate homeostasis) or contain iron as an essential part for catalysis (flavin- and DszA-type monooxygenases, α-ketoglutarate dependent TauD like dioxygenases; Table 1 ).…”