The cephalochordate amphioxus: a key to reveal the secrets of nuclear receptor evolution

Lecroisey, Claire; Laudet, Vincent; Schubert, Michael

doi:10.1093/bfgp/els008

Cited by 29 publications

(24 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, lineage-specific expansions of 10 members of NR1H and three members of NR2E were demonstrated in the cephalochordate Branchiostoma floridae [ 41 ]. Moreover, a massive duplication of HNF4 (>250 HNF4-like NRs) occurred in C. elegans [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of nuclear receptor (NR) superfamily genes in the copepod Tigriopus japonicus

et al. 2014

View full text Add to dashboard Cite

BackgroundNuclear receptors (NRs) are a large superfamily of proteins defined by a DNA-binding domain (DBD) and a ligand-binding domain (LBD). They function as transcriptional regulators to control expression of genes involved in development, homeostasis, and metabolism. The number of NRs differs from species to species, because of gene duplications and/or lineage-specific gene losses during metazoan evolution. Many NRs in arthropods interact with the ecdysteroid hormone and are involved in ecdysone-mediated signaling in arthropods. The nuclear receptor superfamily complement has been reported in several arthropods, including crustaceans, but not in copepods. We identified the entire NR repertoire of the copepod Tigriopus japonicus, which is an important marine model species for ecotoxicology and environmental genomics.ResultsUsing whole genome and transcriptome sequences, we identified a total of 31 nuclear receptors in the genome of T. japonicus. Nomenclature of the nuclear receptors was determined based on the sequence similarities of the DNA-binding domain (DBD) and ligand-binding domain (LBD). The 7 subfamilies of NRs separate into five major clades (subfamilies NR1, NR2, NR3, NR4, and NR5/6). Although the repertoire of NR members in, T. japonicus was similar to that reported for other arthropods, there was an expansion of the NR1 subfamily in Tigriopus japonicus. The twelve unique nuclear receptors identified in T. japonicus are members of NR1L. This expansion may be a unique lineage-specific feature of crustaceans. Interestingly, E78 and HR83, which are present in other arthropods, were absent from the genomes of T. japonicus and two congeneric copepod species (T. japonicus and Tigriopus californicus), suggesting copepod lineage-specific gene loss.ConclusionsWe identified all NR receptors present in the copepod, T. japonicus. Knowledge of the copepod nuclear receptor repertoire will contribute to a better understanding of copepod- and crustacean-specific NR evolution.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-993) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of nuclear receptor (NR) superfamily genes in the copepod Tigriopus japonicus

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Finally, the last group of receptors for which ligand-binding capacities have been studied is the NR1H subfamily (LXR and FXR). Amphioxus possesses 10 paralogues of the FXR/LXR (Farnesoid X Receptor/Liver X Receptor) subfamily due to a lineage-specific duplication event (Lecroisey et al, 2012, Schubert et al, 2008, with one of them being a clear orthologue of vertebrate LXR. Phylogenetic approaches only clarified the orthology of 5 of the other receptors with vertebrate FXRs (Fonseca et al, 2017).…”

Section: Cell-cell Communication Pathways In Amphioxus 707mentioning

confidence: 99%

Developmental cell-cell communication pathways in the cephalochordate amphioxus: actors and functions

Bertrand¹,

Pétillon²,

Somorjai³

et al. 2017

Int. J. Dev. Biol.

View full text Add to dashboard Cite

During embryonic development, cells of metazoan embryos need to communicate in order to construct the correct bodyplan. To do so, they use several signals that usually act through interactions between ligands and receptors. Interestingly, only a few pathways are known to be fundamental during animal development, and they are usually found in all the major metazoan clades, raising the following question: how have evolution of the actors and of the functions of these pathways participated in the appearance of the current diversity of animal morphologies? The chordate lineage comprises vertebrates, their sister group the urochordates, and the cephalochordates (i.e. amphioxus). Urochordates are quite derived relative to the chordate ancestor, whereas cephalochordates and vertebrates share many morphological traits. Thus, comparing embryonic development between vertebrates and cephalochordates should give us some insight into the ancestral characters present in chordates and into the morphological evolution in this clade. However, while much is known about the function of different signalling pathways in vertebrates, data are still scarce in the literature for cephalochordates. In this review, we summarize the current state of the field concerning the expression of actors and the function of the major cell-cell communication pathways, including Hedgehog (Hh), Notch, Nuclear Receptor (NR), Receptor Tyrosine Kinase (RTK), Transforming Growth Factor-β (TGF-β) and Wingless/Int (Wnt), in amphioxus.

show abstract

“…The investigation of the genome sequences from two cephalochordate species allowed the recovery of 10 LXR / FXR -like sequences (NR1H1-10), similar to those found in previous studies (Bertrand et al. 2011; Lecroisey et al. 2012).…”

Section: Introductionmentioning

confidence: 99%

LXRα and LXRβ nuclear receptors evolved in the common ancestor of gnathostomes

et al. 2017

View full text Add to dashboard Cite

Nuclear receptors (NRs) regulate numerous aspects of the endocrine system. They mediate endogenous and exogenous cues, ensuring a homeostatic control of development and metabolism. Gene duplication, loss and mutation have shaped the repertoire and function of NRs in metazoans. Here, we examine the evolution of a pivotal orchestrator of cholesterol metabolism in vertebrates, the liver X receptors (LXRs). Previous studies suggested that LXRα and LXRβ genes emerged in the mammalian ancestor. However, we show through genome analysis and functional assay that bona fide LXRα and LXRβ orthologues are present in reptiles, coelacanth and chondrichthyans but not in cyclostomes. These findings show that LXR duplicated before gnathostome radiation, followed by asymmetric paralogue loss in some lineages. We suggest that a tighter control of cholesterol levels in vertebrates was achieved through the exploitation of a wider range of oxysterols, an ability contingent on ligand-binding pocket remodeling.

show abstract

The cephalochordate amphioxus: a key to reveal the secrets of nuclear receptor evolution

Cited by 29 publications

References 65 publications

Genome-wide identification of nuclear receptor (NR) superfamily genes in the copepod Tigriopus japonicus

Genome-wide identification of nuclear receptor (NR) superfamily genes in the copepod Tigriopus japonicus

Developmental cell-cell communication pathways in the cephalochordate amphioxus: actors and functions

LXRα and LXRβ nuclear receptors evolved in the common ancestor of gnathostomes

Contact Info

Product

Resources

About