The Cervicovaginal Microbiota in Women Notified for<i>Chlamydia trachomatis</i>Infection: A Case-Control Study at the Sexually Transmitted Infection Outpatient Clinic in Amsterdam, The Netherlands

Veer, Charlotte van der; Bruisten, Sylvia M.; Helm, Jürgen; Vries, Henry J. C. de; Houdt, Robin van

doi:10.1093/cid/ciw586

Cited by 67 publications

(87 citation statements)

References 36 publications

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“…L. iners was present in a higher number in C. trachomatis positive women than in healthy controls and this finding is in accordance with previous studies, showing that C. trachomatis positive women were more likely to have a microbiota dominated by L. iners (Ma et al, 2013; van der Veer et al, 2017). This may be explained by the ability of L. iners to survive in a wide range of pH and other metabolic stress-related conditions, adapting to an altered cervico-vaginal environment (Petrova et al, 2017).…”

Section: Discussionsupporting

confidence: 91%

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Diversity of Cervical Microbiota in Asymptomatic Chlamydia trachomatis Genital Infection: A Pilot Study

Filardo¹,

Pietro²,

Porpora

et al. 2017

Front. Cell. Infect. Microbiol.

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Chlamydia trachomatis genital infection continues to be an important public health problem worldwide due to its increasing incidence. C. trachomatis infection can lead to severe sequelae, such as pelvic inflammatory disease, obstructive infertility, and preterm birth. Recently, it has been suggested that the cervico-vaginal microbiota may be an important defense factor toward C. trachomatis infection as well as the development of chronic sequelae. Therefore, the investigation of microbial profiles associated to chlamydial infection is of the utmost importance. Here we present a pilot study aiming to characterize, through the metagenomic analysis of sequenced 16s rRNA gene amplicons, the cervical microbiota from reproductive age women positive to C. trachomatis infection. The main finding of our study showed a marked increase in bacterial diversity in asymptomatic C. trachomatis positive women as compared to healthy controls in terms of Shannon's diversity and Shannon's evenness (P = 0.031 and P = 0.026, respectively). More importantly, the cervical microbiota from C. trachomatis positive women and from healthy controls significantly separated into two clusters in the weighted UniFrac analysis (P = 0.0027), suggesting that differences between the two groups depended entirely on the relative abundance of bacterial taxa rather than on the types of bacterial taxa present. Furthermore, C. trachomatis positive women showed an overall decrease in Lactobacillus spp. and an increase in anaerobes. These findings are part of an ongoing larger epidemiological study that will evaluate the potential role of distinct bacterial communities of the cervical microbiota in C. trachomatis infection.

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Section: Discussionsupporting

confidence: 91%

“…A similar alteration in cervico-vaginal microbiota related to C. trachomatis infection was also found by Ma et al (2013) and van der Veer et al (2017). …”

Section: Discussionsupporting

confidence: 85%

Diversity of Cervical Microbiota in Asymptomatic Chlamydia trachomatis Genital Infection: A Pilot Study

Filardo¹,

Pietro²,

Porpora

et al. 2017

Front. Cell. Infect. Microbiol.

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“…Similar to our findings in CT-negative women, CST-I, CST-III and CST-IV were the most prevalent vaginal microbiota types found in this population, though our study showed a higher proportion of CST-I in non-infected women. This over-representation of CST-I in non-infected women could be explained with an increased protection to STIs by a community dominated by L. crispatus as previously hypothesised by van der Veer et al 8. In their study of a high-risk population for STIs in the Netherlands, proportions of CST-I, CST-III and CST-IV in CT-negative women were 39.0%, 26.8% and 34.2%, respectively.…”

Section: Discussionsupporting

confidence: 54%

Vaginal microbiota composition and association with prevalent Chlamydia trachomatis infection: a cross-sectional study of young women attending a STI clinic in France

Tamarelle

Barbeyrac

Hen³

et al. 2018

Sex Transm Infect

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ObjectivesNew molecular techniques have allowed describing groups of bacterial communities in the vagina (community state types (CST)) that could play an important role in Chlamydia trachomatis (CT) infection. Our aim was to describe the distribution of CST in a population of young women in France.MethodsA cross-sectional study was carried out in June 2015 among anonymous young women attending a STI clinic in Bordeaux, France. Participants provided a vaginal sample for CT screening and sociodemographic data. CT was diagnosed using the Aptima-combo 2 transcription-mediated-amplification assay. Vaginal microbiota composition was characterised using 16S rRNA gene amplicon sequencing.ResultsMicrobiota composition and CT status were available for 132 women. CST dominated by Lactobacillus crispatus (CST-I), L. iners (CST-III) and a diversity of anaerobes (CST-IV) represented 37.1%, 38.6% and 22.0% of the sample, respectively. Twenty-one out of 132 women were CT positive. Proportions of CT-positive women were higher for samples belonging to CST-III (21.6%) and CST-IV (17.2%) than to CST-I (8.2%).ConclusionsFive CST were found in 132 young women from a STI clinic in France. These CSTs were not significantly associated with CT but higher proportions of CT-positive women were found in CST-III and CST-IV, consistent with a previous study in the Netherlands. Though our study lacked statistical power and was cross-sectional, it is a necessary first step to understand the structure of the vaginal microbiota in French women with or without infection before performing in-depth longitudinal studies.

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“…is controversial. Low Lactobacillus levels are one indicator for bacterial vaginosis (BV) and have been associated with increased prevalence, susceptibility and transmission of sexually transmitted infections, including HIV, Neisseria gonorrhoeae, Chlamydia trachomatis and Trichomonas vaginalis [10–14]. However, many asymptomatic women have vaginal bacterial communities that are not dominated by Lactobacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Effects of intrauterine contraception on the vaginal microbiota

et al. 2017

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Objectives There have been conflicting reports of altered vaginal microbiota and infection susceptibility associated with contraception use. The objectives of this study were to determine if intrauterine contraception altered the vaginal microbiota and to compare the effects of a copper intrauterine device (Cu-IUD) and a levonorgestrel intrauterine system (LNG-IUS) on the vaginal microbiota. Study Design DNA was isolated from the vaginal swab samples of 76 women using Cu-IUD (n=36) or LNG-IUS (n=40) collected prior to insertion of intrauterine contraception (baseline) and at 6 months. A third swab from approximately 12 months following insertion was available for 69 (Cu-IUD, n=33; LNG-IUS, n=36) of these women. The V4 region of the bacterial 16S rRNA-encoding gene was amplified from the vaginal swab DNA and sequenced. The 16S rRNA gene sequences were processed and analyzed using the software package mothur to compare the structure and dynamics of the vaginal bacterial communities. Results The vaginal microbiota from individuals in this study clustered into 3 major vaginal bacterial community types: one dominated by Lactobacillus iners, one dominated by Lactobacillus crispatus and one community type that was not dominated by a single Lactobacillus species. Changes in the vaginal bacterial community composition were not associated with the use of Cu-IUD or LNG-IUS. Additionally, we did not observe a clear difference in vaginal microbiota stability with Cu-IUD versus LNG-IUS use. Conclusions Although the vaginal microbiota can be highly dynamic, alterations in the community associated with the use of intrauterine contraception (Cu-IUD or LNG-IUS) were not detected over 12 months. Implications We found no evidence that intrauterine contraception (Cu-IUD or LNG-IUS) altered the vaginal microbiota composition. Therefore, the use of intrauterine contraception is unlikely to shift the composition of the vaginal microbiota such that infection susceptibility is altered.

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The Cervicovaginal Microbiota in Women Notified forChlamydia trachomatisInfection: A Case-Control Study at the Sexually Transmitted Infection Outpatient Clinic in Amsterdam, The Netherlands

Abstract: Women who tested positive for Chlamydia trachomatis infection after having been contact-traced by a chlamydia-positive partner were more likely to have CVM dominated by L. iners or by diverse anaerobic bacteria, than by L. crispatus.

Cited by 67 publications

References 36 publications

Diversity of Cervical Microbiota in Asymptomatic Chlamydia trachomatis Genital Infection: A Pilot Study

Diversity of Cervical Microbiota in Asymptomatic Chlamydia trachomatis Genital Infection: A Pilot Study

Vaginal microbiota composition and association with prevalent Chlamydia trachomatis infection: a cross-sectional study of young women attending a STI clinic in France

Effects of intrauterine contraception on the vaginal microbiota

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