The chain order of binary unsaturated lipid bilayers modulated by aromatic-residue-containing peptides: an ATR-FTIR spectroscopy study

Peng, Bo; Ding, Xiaoyan; Sun, Chao; Yang, Yanan; Gao, Yujiao; Zhao, Xin

doi:10.1039/c7ra01145h

Cited by 5 publications

(2 citation statements)

References 51 publications

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“…There are more than 20 registered modes in this region ( Figure 4 ) arising from the CH 2 , CH 3 and N-H-bending vibrations of different conformations including symmetric and asymmetric bond oscillations in and out of a specified plane, scissoring and waging, etc. [ 28 , 29 , 51 , 52 , 53 , 54 ]. Most of the bands overlap with one another, thus making difficult the exact assignment.…”

Section: Resultsmentioning

confidence: 99%

“…With characteristic diameters comparable to the typical cell sizes (5–100 µm), GUVs serve as a versatile platform for the direct visualization of numerous membrane-related phenomena [ 27 ]. FTIR-ATR measurements of liposome suspensions were carried out to unravel the molecular interactions of the studied peptide sequences with POPC bilayers [ 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

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Interaction of KLAKLAK-NH2 and Analogs with Biomimetic Membrane Models

Vitkova,

Antonova,

Petkov

et al. 2024

Pharmaceutics

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Background: Specifically designed peptide mimetics offer higher selectivity regarding their toxicity to mammalian cells. In addition to the α-helix conformation, the specific activity is related to the peptide’s ability to penetrate the cell membrane. The alterations in lipid membrane properties were addressed in the presence of the peptide KLAKLAK-NH2 and analogs containing β-alanine, strengthening the antibacterial activity and/or naphtalimide with proven anticancer properties. Methods: The molecular interactions of the peptide mimetics with POPC bilayers were studied using FTIR-ATR spectroscopy. The thermal shape fluctuation analysis of quasispherical unilamellar vesicles was applied to probe the membrane bending elasticity. The impedance characteristics of bilayer lipid membranes were measured using fast Fourier-transform electrochemical impedance spectroscopy. Results: A lateral peptide association with the membrane is reported for β-alanine-containing peptides. The most pronounced membrane softening is found for the NphtG-KLβAKLβAK-NH2 analog containing both active groups that corroborate with the indications for 1,8-naphthalimide penetration in the lipid hydrophobic area obtained from the FTIR-ATR spectra analysis. The β-alanine substitution induces strong membrane-rigidifying properties even at very low concentrations of both β-alanine-containing peptides. Conclusions: The reported results are expected to advance the progress in tailoring the pharmacokinetic properties of antimicrobial peptides with strengthened stability towards enzymatic degradation. The investigation of the nonspecific interactions of peptides with model lipid membranes is featured as a useful tool to assess the antitumor and antimicrobial potential of new peptide mimetics.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interaction of KLAKLAK-NH2 and Analogs with Biomimetic Membrane Models

Vitkova,

Antonova,

Petkov

et al. 2024

Pharmaceutics

View full text Add to dashboard Cite

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Effect of phospholipid liposomes on prion fragment (106–128) amyloid formation

Regmi,

Shen,

Stanic

et al. 2023

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Biophysical approaches for exploring lipopeptide-lipid interactions

et al. 2020

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a b s t r a c tIn recent years, lipopeptides (LPs) have attracted a lot of attention in the pharmaceutical industry due to their broad-spectrum of antimicrobial activity against a variety of pathogens and their unique mode of action. This class of compounds has enormous potential for application as an alternative to conventional antibiotics and for pest control. Understanding how LPs work from a structural and biophysical standpoint through investigating their interaction with cell membranes is crucial for the rational design of these biomolecules. Various analytical techniques have been developed for studying intramolecular interactions with high resolution. However, these tools have been barely exploited in lipopeptide-lipid interactions studies. These biophysical approaches would give precise insight on these interactions. Here, we reviewed these state-of-the-art analytical techniques. Knowledge at this level is indispensable for understanding LPs activity and particularly their potential specificity, which is relevant information for safe application. Additionally, the principle of each analytical technique is presented and the information acquired is discussed. The key challenges, such as the selection of the membrane model are also been briefly reviewed.Published by Elsevier B.V. 1.2.

show abstract

The chain order of binary unsaturated lipid bilayers modulated by aromatic-residue-containing peptides: an ATR-FTIR spectroscopy study

Cited by 5 publications

References 51 publications

Interaction of KLAKLAK-NH2 and Analogs with Biomimetic Membrane Models

Interaction of KLAKLAK-NH2 and Analogs with Biomimetic Membrane Models

Effect of phospholipid liposomes on prion fragment (106–128) amyloid formation

Biophysical approaches for exploring lipopeptide-lipid interactions

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