The changes in bone organic and inorganic matrix in newborn rats after maternal application of antiretroviral agents: Indinavir and zidovudine

Maciejewska, Karina; Drzazga, Z.; Kaszuba, Michał

doi:10.1002/biof.1215

Cited by 3 publications

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References 28 publications

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“…The decrease in bone mineral density is one of the most studied adverse effects of prenatal ARV use (Mora et al , ; Amorosa and Tebas, ); however, only one recent study on pregnant rats and their offspring (Maciejewska et al , ) has demonstrated a decrease in calcium ion concentration in bone and teeth, as well as disorders of trace elements, following indinavir administration. Also, a delay in the development of organic dental matrix in teeth of newborn rats of mothers exposed to in utero to zidovudine was demonstrated.…”

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The global burden of oral diseases in pediatric HIV‐infected populations: a workshop report

et al. 2016

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Section: Questionsmentioning

confidence: 99%

The global burden of oral diseases in pediatric HIV‐infected populations: a workshop report

et al. 2016

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Prenatal Zidovudine Treatment Modifies Early Development of Rat Osteoid – Confocal Microspectroscopy Analysis

2019

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Autofluorescence of the bone extracellular matrix (ECM) has not been widely explored although the ECM plays a very important role in bone development. In our research we focused on examining the bone matrix of very young animals due to the intense growth process during the first month of life. Structure images and fluorescence spectra of the bone surface were carried out using confocal fluorescence microscope Eclipse Ti-S inverted CLSM (NIKON, Japan) for compact tibia of healthy 7-, 14- and 28-day-old rat newborns after prenatal zidovudine administration in comparison with control. Spectral features of ECM autofluorescence were analyzed statistically by taking into consideration p < 0.05. The CLSM technique allows for simultaneous examination of the structure and autofluorescence from selected areas of the bone surface. Excessive autofluorescence of ECM after prenatal zidovudine administration influences bone growth incommensurably to the newborns’ age. Therefore the possibility of an additional non-enzymatic mechanism of collagen cross-linking in the first two weeks of life of newborn rats prenatally treated with zidovudine has been considered. Our results suggest that ECM autofluorescence can be an indicator of bone development in the normal and pathological state.

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Serum Autofluorescence and Biochemical Markers in Athlete’s Response to Strength Effort in Normobaric Hypoxia: A Preliminary Study

Drzazga

Schisler

Poprzęcki

et al. 2019

BioMed Research International

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The human organism has the ability to adapt to hypoxia conditions. Training in hypoxia is used in sport to improve the efficiency of athletes; however, type of training affects the direction and scope of this process. Therefore, in this study, the usefulness of serum fluorescence spectroscopy to study the assessment of athlete's response to strength effort in hypoxia is considered in comparison with biochemical assay. Six resistance-trained male subjects took part in a research experiment. They performed barbell squats in simulated normobaric hypoxic conditions with deficiency of oxygen 11.3%, 13% 14.3% compared to 21% in normoxic conditions. Fluorescence intensity of tyrosine revealed high sensitivity on strength effort whereas tryptophan was more dependent on high altitude. Changes in emission in the visible region are associated with altering cell metabolism dependent on high altitude as well as strength training and endurance training. Significant changes in serum fluorescence intensity with relatively weak modifications in biochemical assay at 3000 m above sea level (ASL) were observed. Training at 5000 m ASL caused changes in fluorescence parameters towards the normobaric specific values, and pronounced decreases of lactate level and kinase creatine activity were observed. Such modifications of fluorescence and biochemical assay indicate increased adaptation of the organism to effort in oxygen-deficient conditions at 5000 m ASL, unlike 3000 m ASL. Fluorescence spectroscopy study of serum accompanied by biochemical assay can contribute to the understanding of metabolic regulation and the physiological response to hypoxia. The results of serum autofluorescence during various concepts of altitude training may be a useful method to analyze individual response to acute and chronic hypoxia. An endogenous tryptophan could be exploited as intrinsic biomarker in autofluorescence studies. However, these issues require further research.

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The changes in bone organic and inorganic matrix in newborn rats after maternal application of antiretroviral agents: Indinavir and zidovudine

Cited by 3 publications

References 28 publications

The global burden of oral diseases in pediatric HIV‐infected populations: a workshop report

The global burden of oral diseases in pediatric HIV‐infected populations: a workshop report

Prenatal Zidovudine Treatment Modifies Early Development of Rat Osteoid – Confocal Microspectroscopy Analysis

Serum Autofluorescence and Biochemical Markers in Athlete’s Response to Strength Effort in Normobaric Hypoxia: A Preliminary Study

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