The changing epidemiology of Acinetobacter spp. producing OXA carbapenemases causing bloodstream infections in Brazil: a BrasNet report

Vasconcelos, Ana Tereza Ribeiro de; Barth, Afonso Luís; Zavascki, Alexandre Prehn; Gales, Ana Cristina; Levin, Anna S.; Lucarevschi, Bianca Rezende; Cabral, Blenda Gonçalves; Brasiliense, Danielle Murici; Rossi, Flávia; Furtado, Guilherme Henrique Campos; Carneiro, Irna Carla R.S.; Silva, Juliana O. da; Ribeiro, Julival; Lima, Karla Valéria Batista; Corrêa, Luci; Britto, Maria Helena Marques Fonseca de; Silva, Mariama T.; Conceição, Marília Lima da; Moreira, Marina; Martino, Marinês Dalla Valle; Freitas, Marise Reis de; Oliveira, Maura Salaroli de; DalBen, Mirian Freitas; Guzman, Ricardo D.; Cayô, Rodrigo; Morais, Rosângela; Santos, Sânia Alves dos; Martins, Willames M. B. S.

doi:10.1016/j.diagmicrobio.2015.08.006

Cited by 50 publications

(50 citation statements)

References 19 publications

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“…All carbapenem-resistant A. baumannii isolated in this study harbored the bla OXA-23 gene, as has been also reported by other authors worldwide 10,[32][33][34][35] . The high prevalence of bla OXA-23 may be due to its horizontal transfer, since it has been also identified on plasmids 16,36 .…”

Section: Discussionsupporting

confidence: 56%

“…Resistance to carbapenems has been reported worldwide [9][10][11] , causing special concern because it is the chosen drug to treat infections caused by A. baumannii. Carbapenem resistance in A. baumannii is mainly mediated by the production of class D β-lactamases (oxacillinases) 12 , particularly via the acquisition of genes encoding OXA-23, OXA-24/40, OXA-58, OXA-143, and OXA-51, which are intrinsically located on the chromosome 13,14 .…”

Section: Introductionmentioning

confidence: 99%

“…Carbapenem resistance in A. baumannii is mainly mediated by the production of class D β-lactamases (oxacillinases) 12 , particularly via the acquisition of genes encoding OXA-23, OXA-24/40, OXA-58, OXA-143, and OXA-51, which are intrinsically located on the chromosome 13,14 . The bla OXA-23 gene was originally identified on the chromosome of Acinetobacter radioresistens 15 and is currently widespread in A. baumannii, where it is associated with different genetic structures in the chromosome and plasmids 10,16,17 . In this context, this study evaluated the presence of A. baumannii in an ICU environment and gloves from ICU workers, characterized the antimicrobial resistance of the isolates obtained from different ICU locations, and compared these with clinical isolates from patients in the same ICU.…”

Section: Introductionmentioning

confidence: 99%

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Carbapenem-resistant Acinetobacter baumannii contamination in an intensive care unit

Raro

Gallo

Ferreira

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Introduction: Acinetobacter baumannii is a major pathogen causing infections in intensive care units (ICUs). In this study, we aimed to evaluate the presence of A. baumannii in an ICU environment and gloves from ICU workers and to characterize the antimicrobial resistance of the isolates in comparison with those isolated from ICU patients at the same hospital. Methods: ICU samples were collected from March to November 2010. Isolates biochemically characterized as Acinetobacter calcoaceticusAcinetobacter baumannii complex were evaluated by PCR targeting the 16S rDNA and bla OXA-51 genes. Antimicrobial susceptibility was determined using the disk diffusion method, and carbapenem-resistant isolates were also evaluated for the minimum inhibitory concentration of imipenem using broth microdilution. The presence of the bla OXA-23 gene was evaluated in isolates with reduced susceptibility to carbapenems. Results: A. baumannii was detected in 9.5% (84) of the 886 samples collected from the ICU environment, including from furniture, medical devices, and gloves, with bed rails being the most contaminated location (23.8%; 20/84). Multidrug-resistant (MDR) A. baumannii was found in 98.8% (83/84) of non-clinical and 97.8% (45/46) of clinical isolates. Reduced susceptibility to carbapenems was detected in 83.3% (70/84) of non-clinical and 80.4% (37/46) of clinical isolates. All isolates resistant to carbapenems harbored bla . Conclusions: We found a strong similarity between the antimicrobial susceptibility profiles of non-clinical and clinical A. baumannii isolates. Such data highlight the ICU environment as a potential origin for the persistence of MDR A. baumannii, and hence the ICU may be a source of hospital-acquired infections caused by this microorganism.

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Section: Discussionsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Carbapenem-resistant Acinetobacter baumannii contamination in an intensive care unit

Raro

Gallo

Ferreira

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…OXA-72 is a single-amino-acid variant of OXA-24/40 (G224D). In contrast to OXA-23, which is the most common acquired carbapenemase in A. baumannii worldwide, OXA-72-producing isolates have been reported in only a few countries, including Brazil, Colombia, Taiwan, France, Croatia, Poland, Italy, Lithuania, and Sweden (19,21,(23)(24)(25)(26)(27)(28)(29)(30). The clonal spread of OXA-72-producing A. baumannii isolates was previously reported in the Balkan region, in a Croatian university hospital (24).…”

Section: Discussionmentioning

confidence: 99%

First Occurrence of OXA-72-Producing Acinetobacter baumannii in Serbia

Dortet

Bonnin

Bernabeu

et al. 2016

Antimicrob Agents Chemother

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e Here, we characterized the first OXA-72-producing Acinetobacter baumannii isolate (designated MAL) recovered from a urine sample from a Serbian patient. Antimicrobial susceptibility testing, plasmid analysis, and whole-genome sequencing (WGS) were performed to fully characterize the resistome of the A. baumannii MAL clinical isolate. The isolate was multidrug resistant and remained susceptible only to colistin and tigecycline. PCR analysis revealed the presence of the carbapenemase OXA-72, an OXA-40 variant. Extraction by the Kieser method revealed the presence of two plasmids, and one of these, a ca. 10-kb plasmid, harbored the bla OXA-72 gene. WGS revealed 206 contigs corresponding to a genome of 3.9 Mbp in size with a G؉C content of 38.8%. The isolate belonged to sequence type 492 and to worldwide clone II (WWCII). Naturally occurring ␤-lactamase-encoding genes (bla ADC-25 and bla OXA-66 ) were also identified. Aminoglycoside resistance genes encoding one aminoglycoside adenyltransferase (aadA2), three aminoglycoside phosphatases (strA, strB, aphA6), and one 16S RNA methylase (armA) conferring resistance to all aminoglycosides were identified. Resistance to fluoroquinolones was likely due to mutations in gyrA, parC, and parE. Of note, the resistome matched perfectly with the antibiotic susceptibility testing results.

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“…OXA-23 foi descrita no ano de 1993 em Endimburgo, sendo a primeira oxacilinase a conferir resistência a imipenem identificada em A. baumannii no mundo (Paton et al, 1993 Paulo (Carvalho et al, 2015;de Sá Cavalcanti et al, 2013;Martins e Barth, 2013;Pagano et al, 2017;Vasconcelos et al, 2015;Werneck et al, 2011a) (Figura 2). Unidos, Argentina e Bolívia (Peleg, Seifert e Paterson, 2008;Poirel et al, 2005;Sevillano et al, 2012;Wu et al, 2015).…”

Section: Oxa-23-likeunclassified

Caracterização genética e perfil de sensibilidade antimicrobiana de cepas multirresistentes de Acinetobacter baumannii presentes em um hospital de ensino

Tavares¹

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, que são meu refúgio, minha fonte de energia e personificações do amor em meio às atribulações. AGRADECIMENTOSA Deus e ao plano espiritual, acima de tudo e por tudo.Aos meus avós Ioni e Mário, por todo o suporte, carinho e pela compreensão durante todos esses anos. Sem vocês por perto eu não teria chegado até aqui! Aos meus pais Rosa Maria e Paulo, por sempre terem me provido do necessário e de ainda muito mais a fim de que eu conquistasse todos os objetivos da minha vida e realizasse cada sonho meu. Pelo amor, apoio e carinho incondicionais. Por terem acreditado, assim como eu, que eu poderia chegar até aqui. Não há nada na minha vida que não seja graças a vocês.À minha irmã Ísis por todo o amor, toda a amizade e cumplicidade. Você é a certeza e o conforto de que jamais estarei só nessa grande viagem.A toda a minha família, por ser tão presente e prestativa. Cada telefonema, cada visita, conversa e convite alegram meus dias.Às minhas amigas Ana Paula Cassiolato, Juliana Failde, Juliana Pinhata, Natália Fernandes, Nathamy Fernanda e, especialmente, Gabriela Francisco e Maria Fernanda Bueno por todo auxílio profissional, pessoal e pela nossa amizade que transcende a pós-graduação.Aos amigos Viviane Santana, Cristiane Pinho, Muriel Cestari e Eugênio Telles por todo o apoio e compreensão sempre.Às amigas Fracotes Ana Maria Ruocco, Fernanda Helena Palermo, Juliana Stahl Pinto, Marília Quinalha e Suyen Safuan Naide, irmãs que a vida me deu. É maravilhoso poder acompanhar a evolução e os desdobramentos do caminho de cada uma. Vocês são parte de quem eu sou hoje.Aos amigos Gabriela Fonseca, Letícia Oliveira e Marcelo Corral por terem me recebido tão calorosamente no programa de pós-graduação, dandome a oportunidade de participar ativamente do programa e, principalmente, por toda a ajuda e amizade.Aos companheiros Sérgio Bokermann, Vanda Eliana Garcia Brandolim, Izilda Penha Rodrigues Zuba, Djalma Zuba e todo o Grupo de Amigos, por terem me devolvido tantas vezes ao eixo e me ensinado a ter calma e paciência -ou, ao menos, tentado.Ao meu orientador Carlos Henrique Camargo, pela oportunidade concedida, pelos ensinamentos transmitidos e pela grande inspiração. Por ter acreditado no meu potencial e me dado espaço para crescer, me desenvolver e me descobrir. . ursingii, A. variabilis, A. gyllenbergii (n=1, 0.9% each) and Acinetobacter spp (n=2; 1.7%). A. baumannii isolates were submitted to multiplex PCR for other oxacillinases and ISAba1 detection, antimicrobial susceptibility testing, molecular typing by pulsed field gel electrophoresis (PFGE), trilocus sequence typing (3LST) and multilocus sequence typing (MLST). blaOXA-23-like gene was detected in 105 isolates (92.1%), of which 100% were associated with ISAba1; blaOXA-72 was present in one isolate (0.9%) and blaOXA-231, in two isolates (1.7%). The majority (n=66; 57.9%) of isolates were classified as extensively resistant (XDR). The PFGE grouped the isolates into 11 clusters (A-K) and MLST identified the isolates belonging mainly to CC79 (42.4%), CC1 (16.6%), CC15 (...

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The changing epidemiology of Acinetobacter spp. producing OXA carbapenemases causing bloodstream infections in Brazil: a BrasNet report

Cited by 50 publications

References 19 publications

Carbapenem-resistant Acinetobacter baumannii contamination in an intensive care unit

Carbapenem-resistant Acinetobacter baumannii contamination in an intensive care unit

First Occurrence of OXA-72-Producing Acinetobacter baumannii in Serbia

Caracterização genética e perfil de sensibilidade antimicrobiana de cepas multirresistentes de Acinetobacter baumannii presentes em um hospital de ensino

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