The Chaperone FACT and Histone H2B Ubiquitination Maintain S. pombe Genome Architecture through Genic and Subtelomeric Functions

Murawska, Magdalena; Schauer, Tamás; Matsuda, Atsushi; Wilson, Marcus D.; Pysik, Thomas; Wojcik, Felix; Muir, Tom W.; Hiraoka, Yasushi; Straub, Tobias; Ladurner, Andreas G.

doi:10.1016/j.molcel.2019.11.016

Cited by 36 publications

(55 citation statements)

References 67 publications

(76 reference statements)

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“…While this work was under review, Murawska et al reported a role for H2Bub1 in suppressing antisense transcription in S. pombe , consistent with the findings reported here ( 54 ). Their study indicated that loss of H2Bub1 increased antisense transcription by allowing an aberrant activity of the FACT histone chaperone complex.…”

Section: Discussionsupporting

confidence: 90%

“…At the CPS, reduction in the pSpt5/Spt5 ratio occurs 5′ of a similar reduction in H2Bub1/H2B ratio; the latter effect is a result of nucleosome removal. Loss of H2Bub1 through histone turnover near the CPS was observed in another recent study ( 54 ). This suggests that Spt5 dephosphorylation might trigger chromatin remodeling events leading to H2Bub1 removal at the 3′ ends of genes.…”

Section: Discussionsupporting

confidence: 60%

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Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription

Sansó

Parua

Pinto

et al. 2020

Nucleic Acids Research

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Mono-ubiquitylation of histone H2B (H2Bub1) and phosphorylation of elongation factor Spt5 by cyclin-dependent kinase 9 (Cdk9) occur during transcription by RNA polymerase II (RNAPII), and are mutually dependent in fission yeast. It remained unclear whether Cdk9 and H2Bub1 cooperate to regulate the expression of individual genes. Here, we show that Cdk9 inhibition or H2Bub1 loss induces intragenic antisense transcription of ∼10% of fission yeast genes, with each perturbation affecting largely distinct subsets; ablation of both pathways de-represses antisense transcription of over half the genome. H2Bub1 and phospho-Spt5 have similar genome-wide distributions; both modifications are enriched, and directly proportional to each other, in coding regions, and decrease abruptly around the cleavage and polyadenylation signal (CPS). Cdk9-dependence of antisense suppression at specific genes correlates with high H2Bub1 occupancy, and with promoter-proximal RNAPII pausing. Genetic interactions link Cdk9, H2Bub1 and the histone deacetylase Clr6-CII, while combined Cdk9 inhibition and H2Bub1 loss impair Clr6-CII recruitment to chromatin and lead to decreased occupancy and increased acetylation of histones within gene coding regions. These results uncover novel interactions between co-transcriptional histone modification pathways, which link regulation of RNAPII transcription elongation to suppression of aberrant initiation.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 60%

Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription

Sansó

Parua

Pinto

et al. 2020

Nucleic Acids Research

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“…Despite the low levels of H3K36 methylation, interestingly, knob formation requires Set2, a sole methyltransferase of H3K36 in S. pombe. It has also been shown that the kinetochore protein Sgo2, FACT (facilitates chromatin transcription), which is an essential histone chaperon consisting of Spt16 and Pob3, and the mono ubiquitinated histone H2B (H2Bub) are required for knob formation [102,103]. H2Bub facilitates histone H2A/H2B dimer deposition by FACT.…”

Section: Dapi-dense "Knob" Regionmentioning

confidence: 99%

“…H2Bub facilitates histone H2A/H2B dimer deposition by FACT. In addition, FACT maintains the nucleosome density in the sub-telomeric region [103], which may be the key to knob formation. The border between the heterochromatin and euchromatin is also controlled by the anti-silencing factor, Epe1 [90,91].…”

Section: Dapi-dense "Knob" Regionmentioning

confidence: 99%

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Nuclear Envelope Proteins Modulating the Heterochromatin Formation and Functions in Fission Yeast

Hirano

Asakawa

Sakuno

et al. 2020

Cells

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The nuclear envelope (NE) consists of the inner and outer nuclear membranes (INM and ONM), and the nuclear pore complex (NPC), which penetrates the double membrane. ONM continues with the endoplasmic reticulum (ER). INM and NPC can interact with chromatin to regulate the genetic activities of the chromosome. Studies in the fission yeast Schizosaccharomyces pombe have contributed to understanding the molecular mechanisms underlying heterochromatin formation by the RNAi-mediated and histone deacetylase machineries. Recent studies have demonstrated that NE proteins modulate heterochromatin formation and functions through interactions with heterochromatic regions, including the pericentromeric and the sub-telomeric regions. In this review, we first introduce the molecular mechanisms underlying the heterochromatin formation and functions in fission yeast, and then summarize the NE proteins that play a role in anchoring heterochromatic regions and in modulating heterochromatin formation and functions, highlighting roles for a conserved INM protein, Lem2.

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New connections between ubiquitylation and methylation in the co-transcriptional histone modification network

et al. 2021

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The Chaperone FACT and Histone H2B Ubiquitination Maintain S. pombe Genome Architecture through Genic and Subtelomeric Functions

Abstract: The chaperone FACT and histone H2B ubiquitination maintain S. pombe genome architecture through genic and subtelomeric functions

Cited by 36 publications

References 67 publications

Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription

Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription

Nuclear Envelope Proteins Modulating the Heterochromatin Formation and Functions in Fission Yeast

New connections between ubiquitylation and methylation in the co-transcriptional histone modification network

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