The characterisation of the lymphoma cell line U937, using comparative genomic hybridisation and multi-plex FISH

Abstract

The cell line U937, which has been used extensively for studies of myeloid differentiation, bears the t(10;11)(p13;q14) translocation which results in a fusion between the MLLT10 (myeloid/lymphoid or mixed-lineage leukemia [trithorax, Drosophila, homolog]; translocated to 10; alias AF10) gene and the Ap-3-like clathrin assembly protein, PICALM (Clathrin assembly lymphoid myeloid leukaemia). Apart from this translocation, very little is known about the other genetic alterations in this cell line that may represent significant events in disease progression. In this study, conventional G-banding, CGH and M-FISH have been used to characterise fully all of the cytogenetic alterations present in the U937 cell line. M-FISH analysis confirmed the presence of the t(10;11) and an apparently normal copy of both chromosomes 10 and 11. A t(1;5) translocation was observed as well as several unbalanced rearrangements. CGH detected amplifications resulting from duplications of 2q, 6p and 13q. These changes could result in fusion gene products involved in carcinogenesis or the positions of putative oncogenes and tumour suppressor genes. A good correlation between conventional G-banding, CGH and M-FISH was observed.

NF-?B translocation and endothelial cell activation is potentiated by macrophage-released signals co-secreted with TNF-? and IL-1?

“…On the other hand, U937 cells have been widely used as an in vitro model for myeloid differentiation and activation. Furthermore, U937 cells are an excellent source of mature human macrophage-like cells since they express the typical myeloid markers after in vitro differentiation [23][24][25][26].…”

Comparative M-FISH and CGH analyses in sensitive and drug-resistant human T-cell acute leukemia cell lines

“…While a huge number of publications have addressed the chromosomal characterization of human solid tumors (Pandita et al, 1999;Bayani et al, 2000Bayani et al, , 2002Naus et al, 2001;Scheil et al, 2001;Squire et al, 2001Squire et al, , 2002Wang et al, 2001) and solid tumor cell lines by CGH and M-FISH (Ghadimi et al, 2000;Kytölä et al, 2000;Mairal et al, 2000;Speicher et al, 2000;Abdel-Rahman et al, 2001;Luk et al, 2001;Micci et al, 2001;Tsushimi et al, 2001;van Roy et al, 2001;Lensch et al, 2002;van Gele et al, 2002;Xie et al, 2002), only a few studies have applied these techniques in combination to single leukemia cell lines (Tosi et al, 1999;Gribble et al, 2000;LindbjergAndersen et al, 2000;Strefford et al, 2001;Naumann et al, 2001).…”

“…This is true for leukemic cell lines (Tosi et al, 1999;Gribble et al, 2000;Lindbjerg-Andersen et al, 2000;Naumann et al, 2001;Strefford et al, 2001) as well as cell lines established from solid human tumors (Ghadimi et al, 2000;Kytölä et al, 2000;Speicher et al, 2000;Abdel-Rahman et al, 2001;Luk et al, 2001;Squire et al, 2001;Tsushimi et al, 2001;van Roy et al, 2001). CGH detects genome-wide imbalances in one single analysis (Gebhart and Liehr, 2000), while M-FISH is particularly suited to find both balanced and imbalanced interchromosomal rearrangements (Schröck et al, 1996;Speicher et al, 1996).…”

Linker for Activation of T-cell Family Member2 (LAT2) a Lipid Raft Adaptor Protein for AKT Signaling, Is an Early Mediator of Alkylphospholipid Anti-leukemic Activity

“…Cell Culture and Viability Measurements-The human cell lines NB4 (acute promyelocytic leukemia) (16), U937 (histiocytic lymphoma with myeloid markers) (17), and K562 (chronic myeloid leukemia in blast crisis) (18) were cultured at 37°C with 5% CO 2 in RPMI 1640 medium supplemented with 10% fetal bovine serum. Cell viability was determined via trypan blue assay, and only cultures with Ն95% viability were used.…”