The characteristics of a canine mammary carcinoma cell line, REM 134

Else, R. W.; Norval, Mary; Neill, William A.

doi:10.1038/bjc.1982.254

Cited by 33 publications

(27 citation statements)

References 15 publications

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“…Else, The University of Edinburgh, UK) [33] were grown in Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, Paisley, UK) supplemented with 10% fetal bovine serum and 100 μg/mL streptomycin (Invitrogen, Paisley, UK). For anchorage independent culture, REM134 cells were plated as single cells in ultralow attachment 6-well plates (Corning, CA, USA) at low cell density (1.5 × 10 4 cells/mL).…”

Section: Methodsmentioning

confidence: 99%

Canine Mammary Cancer Stem Cells are Radio- and Chemo- Resistant and Exhibit an Epithelial-Mesenchymal Transition Phenotype

Pang

Cervantes-Arias

Else

et al. 2011

Cancers

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Canine mammary carcinoma is the most common cancer among female dogs and is often fatal due to the development of distant metastases. In humans, solid tumors are made up of heterogeneous cell populations, which perform different roles in the tumor economy. A small subset of tumor cells can hold or acquire stem cell characteristics, enabling them to drive tumor growth, recurrence and metastasis. In veterinary medicine, the molecular drivers of canine mammary carcinoma are as yet undefined. Here we report that putative cancer stem cells (CSCs) can be isolated form a canine mammary carcinoma cell line, REM134. We show that these cells have an increased ability to form tumorspheres, a characteristic of stem cells, and that they express embryonic stem cell markers associated with pluripotency. Moreover, canine CSCs are relatively resistant to the cytotoxic effects of common chemotherapeutic drugs and ionizing radiation, indicating that failure of clinical therapy to eradicate canine mammary cancer may be due to the survival of CSCs. The epithelial to mesenchymal transition (EMT) has been associated with cancer invasion, metastasis, and the acquisition of stem cell characteristics. Our results show that canine CSCs predominantly express mesenchymal markers and are more invasive than parental cells, indicating that these cells have a mesenchymal phenotype. Furthermore, we show that canine mammary cancer cells can be induced to undergo EMT by TGFβ and that these cells have an increased ability to form tumorspheres. Our findings indicate that EMT induction can enrich for cells with CSC properties, and provide further insight into canine CSC biology.

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Section: Methodsmentioning

confidence: 99%

Canine Mammary Cancer Stem Cells are Radio- and Chemo- Resistant and Exhibit an Epithelial-Mesenchymal Transition Phenotype

Pang

Cervantes-Arias

Else

et al. 2011

Cancers

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“…We could not establish CMC cell lines from other specimens, possibly because of fibroblasts contamination, bacterial contamination or unknown reasons. To date, other studies have established malignant CMC cell lines; 29–34 however, plural cell pairs of primary and metastatic origin have not been reported thus far.…”

Section: Discussionmentioning

confidence: 99%

Establishment of four pairs of canine mammary tumour cell lines derived from primary and metastatic origin and their E‐cadherin expression

Uyama

Nakagawa

Hong

et al. 2006

Vet Comparative Oncology

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Four new pairs of canine mammary carcinoma cell lines derived from both primary and metastatic lesions were established. The cells were cultured in RPMI-1640 with 10% fetal bovine serum and they showed stable growth for more than 120 passages. Using these cell lines, the expression of E-cadherin was measured by flow cytometry and the function of E-cadherin was evaluated by cell aggregation assay and results from the primary and metastatic lesions were compared statistically. E-cadherin was strongly expressed in all of the cell lines, without a notable difference between cells of primary and metastatic origin. In the cell aggregation assay, the function of E-cadherin was significantly weaker in the cells of primary origin (p < 0.05), as compared with cells of metastatic origin. The present results suggest that a reduction in E-cadherin function may be implicated in the invasive and metastatic potential of canine mammary tumour cells; however, further study will be needed to clarify E-cadherin function in the context of the metastasis of canine mammary carcinoma.

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“…The human osteosarcoma cell line U2OS was grown in McCoys 5A modified medium (ThermoFisher Scientific). Canine mammary carcinoma cells REM134 (REM) were maintained in DMEM. Canine inflammatory mammary carcinoma cells LILY (gifted from Dr De Maria Raffaella, University of Turin) were grown in RPMI medium 1640 (ThermoFisher Scientific) supplemented with 10 μg/mL human insulin (Sigma‐Aldrich, Missouri) and 10 ng/mL human recombinant EGF (Peprotech, London, UK).…”

Section: Methodsmentioning

confidence: 99%

The selective cyclooxygenase‐2 inhibitor mavacoxib (Trocoxil) exerts anti‐tumour effects in vitro independent of cyclooxygenase‐2 expression levels

Hurst

Pang

Argyle

2019

Vet Comparative Oncology

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The inducible inflammatory enzyme cyclooxygenase‐2 (COX‐2) and its product prostaglandin E2 (PGE2) are prominent tumour promoters, and expression of COX‐2 is elevated in a number of tumours of both humans and canines. Targeting COX‐2 in cancer is an attractive option because of readily available non‐steroidal anti‐inflammatory drugs (NSAIDs), and there is a clear epidemiological link between NSAID use and cancer risk. In this study, we aim to establish the anti‐tumourigenic effects of the selective, long‐acting COX‐2 inhibitor mavacoxib. We show here that mavacoxib is cytotoxic to a panel of human and canine osteosarcoma, mammary and bladder carcinoma cancer cell lines; that it can induce apoptosis and inhibit the migration of these cells. Interestingly, we establish that mavacoxib can exert these effects independently of elevated COX‐2 expression. This study highlights the potential novel use of mavacoxib as a cancer therapeutic, suggesting that mavacoxib may be an effective anti‐cancer agent independent of tumour COX‐2 expression.

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The characteristics of a canine mammary carcinoma cell line, REM 134

Abstract: Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 p679-b Fig. 8

Cited by 33 publications

References 15 publications

Canine Mammary Cancer Stem Cells are Radio- and Chemo- Resistant and Exhibit an Epithelial-Mesenchymal Transition Phenotype

Canine Mammary Cancer Stem Cells are Radio- and Chemo- Resistant and Exhibit an Epithelial-Mesenchymal Transition Phenotype

Establishment of four pairs of canine mammary tumour cell lines derived from primary and metastatic origin and their E‐cadherin expression

The selective cyclooxygenase‐2 inhibitor mavacoxib (Trocoxil) exerts anti‐tumour effects in vitro independent of cyclooxygenase‐2 expression levels

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