The characterization of superoxide production of human neonatal neutrophil

Komatsu, Hajime; Tsukimori, Kiyomi; Hata, Ken‐ichiro; Satoh, Shoji; Nakano, Hitoo

doi:10.1016/s0378-3782(01)00188-8

Cited by 31 publications

(24 citation statements)

References 24 publications

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“…Labor is associated with the generation of inflammatory cytokines, including IL-6 and IL-8, which are known to stimulate the production of ROIs in neutrophils (33). These findings are consistent with previous reports that the oxidative response to inflammatory mediators is impaired in term neonatal neutrophils (34,35). Decreased responsiveness to inflammatory stimuli may be related to defects in the activation of signaling via the nuclear transcription factors STAT-1 and NF-B in neonatal cells (36).…”

Section: Discussionsupporting

confidence: 88%

Influence of Labor on Neonatal Neutrophil Apoptosis, and Inflammatory Activity

et al. 2007

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Section: Discussionsupporting

confidence: 88%

Influence of Labor on Neonatal Neutrophil Apoptosis, and Inflammatory Activity

et al. 2007

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“…PMA is a soluble stimulus that acts directly on protein kinase C (present in cytoplasm) activating the production of the superoxide anion, which is subsequently converted to H 2 O 2 (17). PMA-induced stimulation can be mediated by specific receptors, and also via insertion into the membrane due to its hydrophobic nature (18).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity of chlorhexidine digluconate to murine macrophages and its effect on hydrogen peroxide and nitric oxide induction

Bonacorsi

Raddi

Carlos

2004

Braz J Med Biol Res

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Chlorhexidine, even at low concentrations, is toxic for a variety of eukaryotic cells; however, its effects on host immune cells are not well known. We evaluated in vitro chlorhexidine-induced cytotoxicity and its effects on reactive oxygen/nitrogen intermediate induction by murine peritoneal macrophages. Thioglycollate-induced cells were obtained from Swiss mice by peritoneal lavage with 5 ml of 10 mM phosphate-buffered saline, washed twice and resuspended (10 6 cells/ ml) in appropriate medium for each test. Cell preparations contained more than 95% macrophages. The cytotoxicity was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the presence of hydrogen peroxide (H 2 O 2 ) and nitric oxide (NO) by the horseradish peroxidase-dependent oxidation of phenol red and Griess reaction, respectively. The midpoint cytotoxicity values for 1-and 24-h exposures were 61.12 ± 2.46 and 21.22 ± 2.44 µg/ml, respectively. Chlorhexidine did not induce synthesis or liberation of reactive oxygen/nitrogen intermediates. When macrophages were treated with various sub-toxic doses for 1 h (1, 5, 10, and 20 µg/ml) and 24 h (0.5, 1, and 5 µg/ml) and stimulated with 200 nM phorbol myristate acetate (PMA) solution, the H 2 O 2 production was not altered; however, the NO production induced by 10 µg/ml lipopolysaccharide (LPS) solution varied from 14.47 ± 1.46 to 22.35 ± 1.94 µmol/ l and 13.50 ± 1.42 to 20.44 ± 1.40 µmol/l (N = 5). The results showed that chlorhexidine has no immunostimulating activity and sub-toxic concentrations did not affect the response of macrophages to the soluble stimulus PMA but can interfere with the receptor-dependent stimulus LPS. Correspondence

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“…Details of the CL assay have been described previously. 9 Plasma was isolated from the cord blood before neutrophil preparation and centrifuged at 3000g for 20 minutes at 4˚C. Aliquots of plasma were filtrated with a 22µm filter (MilliPore Corporation, Bedford, MA, USA) and stored at -80˚C until each test was conducted.…”

Section: Methodsmentioning

confidence: 99%

Increased inflammatory markers are associated with early periventricular leukomalacia

Tsukimori

Komatsu

Yoshimura

et al. 2007

Develop Med Child Neuro

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The aim of the study was to investigate whether inflammatory markers are associated with the occurrence of periventricular leukomalacia (PVL). Superoxide (O2‐) production of neutrophils and plasma antioxidative superoxide dismutase (SOD) activity in umbilical cord blood were studied. Participants were preterm infants with early PVL (n=6; three males, three females; mean birthweight 1458g [SD 517], range 620–2040g; mean gestational age 29.8wks [SD 2.9], range 27–34wks); and preterm control infants without PVL (n=10; five males, five females; mean birthweight 1838g [SD 664], range 925–2748g; mean gestational age 30.6wks [SD 3.1], range 26–34wks). In addition, pro‐inflammatory cytokine levels were measured in the umbilical cord blood. N‐formyl‐methionyl‐leucyl‐phenylalanine‐induced O2‐ production by neutrophils in infants with early PVL was significantly higher than that in the control group. In contrast, there was no significant difference in concentrations of copper/zinc‐SOD and SOD activity between groups. Concentrations of interleukin (IL)‐1β and tumour necrosis factor‐α (but not IL‐6, IL‐8, or granulocyte‐colony stimulating factor) were significantly higher in infants with early PVL than in control infants. The excess O2‐ produced by activated neutrophils with increased pro‐inflammatory cytokine production could play a role in the molecular cascade leading to white matter damage in PVL.

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The characterization of superoxide production of human neonatal neutrophil

Cited by 31 publications

References 24 publications

Influence of Labor on Neonatal Neutrophil Apoptosis, and Inflammatory Activity

Influence of Labor on Neonatal Neutrophil Apoptosis, and Inflammatory Activity

Cytotoxicity of chlorhexidine digluconate to murine macrophages and its effect on hydrogen peroxide and nitric oxide induction

Increased inflammatory markers are associated with early periventricular leukomalacia

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