The Characterization of the<i>Caenorhabditis elegans</i>Mitochondrial Thioredoxin System Uncovers an Unexpected Protective Role of Thioredoxin Reductase 2 in β-Amyloid Peptide Toxicity

Cacho-Valadez, Briseida; Muñoz-Lobato, Fernando; Pedrajas, José Rafael; Cabello, Juan; Fierro-González, Juan Carlos; Navas, Plácido; Swoboda, Peter; Link, Chris D.; Miranda‐Vizuete, Antonio

doi:10.1089/ars.2011.4265

Cited by 46 publications

(55 citation statements)

References 61 publications

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“…Oxidative stress has been thought to contribute to the general decline in cellular functions, and is associated with a number of human diseases including AD (Cacho-Valadez et al 2012). AD is one of the most common neurodegenerative diseases of humans.…”

Section: Introductionmentioning

confidence: 99%

Omega-3 fatty acid obtained from Nannochloropsis oceanica cultures grown under low urea protect against Abeta-induced neural damage

Lai

2014

J Food Sci Technol

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Amyloid-beta (Abeta) protein is a key factor in the pathogenesis of Alzheimer's disease (AD). Moreover, it has been reported that oxidative stress is involved in the biochemical pathway by which Abeta can lead to neuronal dysfunction. Recently, docosahexaenoic acid (DHA; C22:6) and eicosapentaenoic acid (EPA; C20:5n-3) have been reported to protect against AD. However, these omega-3 fatty acids are frequently obtained from fish oil and may contain heavy metals. In this study, we utilized Nannochloropsis oceanica to produce omega-3 fatty acid. We observed that when urea levels (nitrogen source) were lowered from 2 to 0.2 g/L in Nannochloropsis oceanica cultures, EPA production increased. Moreover, EPA in Nannochloropsis oceanica effectively promoted antioxidant activity to counter the Abetainduced oxidative stress in Neuro-2A cells. These results indicate that Nannochloropsis oceanica may be potentially used as a therapeutic agent or as a functional food that promotes protection against AD.

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Section: Introductionmentioning

confidence: 99%

Omega-3 fatty acid obtained from Nannochloropsis oceanica cultures grown under low urea protect against Abeta-induced neural damage

Lai

2014

J Food Sci Technol

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“…65,77 Interestingly, only the knockdown of trxr-2 increased the paralysis onset of the Ab-expressing model, while depletion of trx-2 had no effect. 65 These data indicate an important role of the mitochondrial thioredoxin reductase trxr-2 in Ab proteotoxicity. Using a cellular model of Parkinson disease (PD), a loss of TrxR2 (mammalian homolog of trxr-2) activity was monitored.…”

Section: Proteins Of the Thioredoxin Network Play Important Roles Inmentioning

confidence: 97%

“…However, only limited information is present about their function and characteristics. 12,[62][63][64][65][66][67] Thioredoxins are highly specialized in their tissue, cell and compartmental expression pattern (Fig. 1).…”

Section: The Crucial Role Of Pdi's and Ero-1 To Maintain Er Homeostasmentioning

confidence: 99%

“…A protective role of TRXR-2 was reported in an Alzheimer disease (AD) model of C. elegans, which expresses Ab 1-42 in muscle cells. 65 TRXR-2 and its thioredoxin TRX-2 are expressed in mitochondria in several tissues and are up-regulated upon mitochondrial stress. 65,77 Interestingly, only the knockdown of trxr-2 increased the paralysis onset of the Ab-expressing model, while depletion of trx-2 had no effect.…”

Section: Proteins Of the Thioredoxin Network Play Important Roles Inmentioning

confidence: 99%

“…65 TRXR-2 and its thioredoxin TRX-2 are expressed in mitochondria in several tissues and are up-regulated upon mitochondrial stress. 65,77 Interestingly, only the knockdown of trxr-2 increased the paralysis onset of the Ab-expressing model, while depletion of trx-2 had no effect. 65 These data indicate an important role of the mitochondrial thioredoxin reductase trxr-2 in Ab proteotoxicity.…”

Section: Proteins Of the Thioredoxin Network Play Important Roles Inmentioning

confidence: 99%

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Interplay between redox and protein homeostasis

Feleciano

Arnsburg

Kirstein

2016

Worm

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The subcellular compartments of eukaryotic cells are characterized by different redox environments. Whereas the cytosol, nucleus and mitochondria are more reducing, the endoplasmic reticulum represents a more oxidizing environment. As the redox level controls the formation of intra-and inter-molecular disulfide bonds, the folding of proteins is tightly linked to its environment. The proteostasis network of each compartment needs to be adapted to the compartmental redox properties. In addition to chaperones, also members of the thioredoxin superfamily can influence the folding of proteins by regulation of cysteine reduction/oxidation. This review will focus on thioredoxin superfamily members and chaperones of C. elegans, which play an important role at the interface between redox and protein homeostasis. Additionally, this review will highlight recent methodological developments on in vivo and in vitro assessment of the redox state and their application to provide insights into the high complexity of redox and proteostasis networks of C. elegans.

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Detection of Mitochondrial Toxicity of Environmental Pollutants UsingCaenorhabditis elegans

Maurer

Luz

Meyer

2018

Mitochondrial Dysfunction Caused by Drugs and Environmental Toxicants

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The Characterization of theCaenorhabditis elegansMitochondrial Thioredoxin System Uncovers an Unexpected Protective Role of Thioredoxin Reductase 2 in β-Amyloid Peptide Toxicity

Abstract: The nematode strains characterized in this work make C. elegans an ideal model organism to study the pathophysiology of the mitochondrial thioredoxin system at the level of a complete organism.

Cited by 46 publications

References 61 publications

Omega-3 fatty acid obtained from Nannochloropsis oceanica cultures grown under low urea protect against Abeta-induced neural damage

Omega-3 fatty acid obtained from Nannochloropsis oceanica cultures grown under low urea protect against Abeta-induced neural damage

Interplay between redox and protein homeostasis

Detection of Mitochondrial Toxicity of Environmental Pollutants UsingCaenorhabditis elegans

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