The charged milieu: a major player in fertilization reactions

Maldonado, Marcela; Weerasinghe, Gayani; Ambroise, Fabienne; Yamoah, E. H.; Londono, Monica; Pelayo, Juan C.; Grigorian, Melina; Oppenheimer, Steven B.

doi:10.1016/j.acthis.2003.10.004

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2008

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“…From these results it can be suggested that D-glucose under physiological concentration (1mM to 5mM) supports sperm capacitation that is essential for fertilization. On the other hand D-fructose (0.05mM) is required for sperm motility and sperm-oocyte fusion [30,31]. The decrease in aggregation of HBPs in presence of D-fructose may cause increase in sperm motility.…”

Section: Discussionmentioning

confidence: 98%

Comparative Studies on the Aggregation Behavior of HBPs from Human Seminal Plasma by Dynamic Light Scattering

Kumar

Hassan²,

Singh³

et al. 2008

PPL

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Heparin binding proteins (HBPs) from human seminal plasma (HSP) were obtained by heparin affinity and size exclusion chromatography. The aggregation/disaggregation of HBPs was followed by dynamic light scattering (DLS) in presence of various physiological ligands such as CaCl(2), NaCl, EDTA, cholesterol, adenosine, D-glucose and D-fructose. The aggregation pattern of lactoferrin was also analyzed and compared. The study of interactions of HBPs of HSP may contribute to an understanding mechanisms underlying the fertilization process.

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Section: Discussionmentioning

confidence: 98%