The chemical characterization of a major allergen of timothy pollen

Human Immune Response against Timothy Grass Pollen Allergens

“…1) not only con firms earlier data indicating its participation in the antigenic determinant of AgB [21,22], but also sug gests that cellobiose is directly involved in the binding site of the antigenic determinant. Reth et al [7] and Kranz and Voss [24], analyzing various monoclonal anti-ld, have suggested that inhibition of idiotypeanti-Id interaction by free hapten represents direct binding of the hapten with the idiotope and that inhi bition with a hapten-protein conjugate indicates the binding of the hapten at sites adjacent to the idiotope.…”

“…Previous structural analysis of AgD, a monovalent antigen fragment of AgB, demonstrated that cello biose represents a major part of the antigenic determi nant of AgB [21]. Consequently, we used cellobiose, and varying concentrations of a variety of other di saccharides and salicin, to block the binding of anti-Id with AgB-specific IgE ( fig.…”

“…The cells were harvested and counted by liquid scin tillation methods. The stimulation index was calculated as: experimental cpm -background cpm background cpm induced histamine release from monkey mast cells [21,22], Those studies showed that monkey mast cells passively sensitized with serum from timothy-sensi tive patients show a linear, dose-dependent inhibition of antigen-induced histamine release upon the addi tion of AgD prior to the WST challenge. These earlier observations and our more recent findings raise a question regarding the possible conservation of the idiotypic determinant expressed by murine AgB-spe cific lymphocyte receptors and the idiotypic determi nant expressed by human AgB-specific IgE.…”

Isolation and Partial Characterization of Allergen C from Kentucky Blue Grass Pollen

“…possessed essentially all of the aller genic determinants collectively present in R. The studies of Malley ct al. [11] have also suggested that the principal allergenic spe cificity of the aqueous extract of Timothy grass pollen was born by a well-defined al lergen referred to as D3, which was com posed of flavonoid pigment, a sugar moiety of cellobiose and at least one amino acid, threonine.…”

Induction and Characterization of Suppressor T Cells and Soluble Factors with Modified Timothy Grass Pollen AgB

“…Timothy pollen extract (WST), purified AgB, Ox-AgB, antigen D, (AgD,), and keyhole limpet (Megatliura crenulata) hemocyanin (KLH) were prepared as described elsewhere [18][19][20][21], The pro tein content of the samples was determined by the method of Lowry [22], The carbohydrate content of the suppressor factor was described by the orcinol method [23].…”