The Chemical Composition of Chlorella; Effect of Environmental Conditions

Spoehr, H. A.; Milner, Harold W.

doi:10.1104/pp.24.1.120

Cited by 364 publications

(96 citation statements)

References 9 publications

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“…A corresponding increase in carbohydrate content may be inferred from calculations based on the equations of Spoehr and Milner (20). As nitrite production proceeded, the phycocyanin content of the cells was drastically reduced (Table II), but, significantly, the chlorophyll a content remained constant.…”

Section: Methodsmentioning

confidence: 99%

Characteristics of Nitrate Reduction in a Mutant of the Blue-Green Alga Agmenellum quadruplicatum

Stevens¹,

Baalen²

1973

Plant Physiol.

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Characteristics of nitrate reduction in terms of nitrite production in an N-methyl-N'-nitro-N-nitrosoguanidine-induced mutant of the blue-green alga Agmenellum quadruplicatum are described. Following induction of nitrate reduction a linear rate of nitrite production proportional to cell concentration was observed. Rate of nitrite production and growth rate showed similar responses to pH, temperature, and light intensity. If required, only trace amounts of carbon dioxide were necessary for nitrite production. Atmospheres of oxygen or nitrogen inhibited production of nitrite. In addition, a low but constant rate of nitrite production was observed in the dark. Nitrite production by mutant AQ-6 was studied in terms of photosynthesis.As nitrite production proceeded, rate of photosynthesis declined. Ultraviolet irradiation and 3-(3,4-dichlorophenyl)-1, 1-dimethylurea poisoning did not prevent nitrite production. The action spectrum of nitrite production was chlorophyll a-like.Recently, most studies of nitrate reduction in algae have been concerned with the interpretation of data obtained from cell-free studies (4,6,7,12,23,31). Herein, we report on a different approach to the study of nitrate reduction. Previously, we have characterized primarily via growth experiments a series of mutants derived from the coccoid, marine blue-green alga Agmenellum qtuadruplicatum strain 22). Several of these mutants were impaired in their ability to utilize nitrate as sole nitrogen source. One of these mutants, namely strain AQ-6, was selected for use in the study of the long-standing problem of the connection, if any, between photosynthesis and nitrate reduction (28). AQ-6 was impaired in its ability to carry out the reduction of nitrite to cellular nitrogen, but it was unimpaired in its ability to reduce nitrate to nitrite. Thus, nitrite accumulated in the medium and was readily assayable. In this paper we report the results of our beginning study of the connection between photosynthesis and nitrate reduction. MATERIALS AND METHODSOrganism, Media, and Growth. A. quadruplicatum strain PR-6 (hereinafter called PR-6) is a marine isolate of this lab

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Section: Methodsmentioning

confidence: 99%

Characteristics of Nitrate Reduction in a Mutant of the Blue-Green Alga Agmenellum quadruplicatum

Stevens¹,

Baalen²

1973

Plant Physiol.

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“…For the many interest ing observations made by Hase and co-workers (43, 45-48, 50, 52) on feeding the S-starved cells with 35S-sulfate under various environmental conditions, see the reviews by Hase (41) and Tamiya (200,201). Otsuka (130) found with C. ellipsoidea that in the S-deficiency [as also known to be true for the case of N-deficiency (192)], carbohydrates were converted into fatty acids and lipids [see also (102)], whose composition was different from that of nor mally grown cells, i.e., lacking the sulfoJipid discovered by Benson, Daniel & Wiser (10) in normal photoautotrophic cells in general. The supply of the S source alone to the S-starved cells was effective in restoring the lower level of carbohydrates, but not in decreasing the abnormally elevated level of lipids.…”

Section: Modifications Caused By Various Unphysiological Factorsmentioning

confidence: 99%

Synchronous Cultures of Algae

Tamiya¹

1966

Annu. Rev. Plant. Physiol.

122

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“…There are several suggested advantages of microalgae. If algae are grown under suitable environment conditions, the protein yield from it may be quite better (Spoehr and Milner, 1949). In China and Japan, Int.…”

Section: Introductionmentioning

confidence: 99%

Culture of Chlorella ellipsoidea in different culture media

Mohshina¹,

Shahjahan²,

Chowdhury³

et al. 2017

Int. J. Agril. Res. Innov. & Tech.

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An experiment of algal culture was conducted in natural light and temperature conditions at a balcony of a room at the 2 nd floor of Fisheries Faculty Building facing the north. The experiment was done to evaluate the growth of Chlorella ellipsoidea in four different media, viz, medium I (inorganic), medium II (organic, whole pulse powder extract), medium III (organic, whole lentil powder extract) and medium IV (organic, whole gram powder extract) under natural environment conditions during January-June, 2015. Growth rates of the algal species in four different media were found not significantly different. The alga, C. ellipsoidea attained maximum cell density of 28.89×10 6 cell ml -1 in the 15 th day in medium I, of 30.69×10 6 cell ml -1 in the 13 th day in medium II, of 26.18×10 6 cell ml -1 in the 15 th day in medium III and of 21.12×10 6 cell ml -1 in the 13 th day in medium IV. The ranges of air temperature, water temperature and light intensity were 21°C to 38°C, 23°C to 36°C and 2.28×10 3 to 9.60×10 3 Lux respectively during the culture period. The average sunshine period was 5.87±2.82 hrs. Total alkalinity, free CO2, pH , NO3-N and PO4-P of algal culture media I, II, III and IV were 128, 540, 554 and 322 mgL -1 ; 32, 162, 102, 70 mgL -1 ; 7.4, 8, 7.9 and 7.9; 180, 36.6, 62.4 and 150 mgL -1 , and 25.2, 48.2, 42.4 and 45.6 mgL -1 , respectively. According to ANOVA of cell densities of cultures of C. ellipsoidea under treatments are not significantly different (F=1.441077). It is clear that differences between them are not significant i.e. mean algal cell densities are more or less same as differences between treatments are less than 20%.

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The Chemical Composition of Chlorella; Effect of Environmental Conditions

Cited by 364 publications

References 9 publications

Characteristics of Nitrate Reduction in a Mutant of the Blue-Green Alga Agmenellum quadruplicatum

Characteristics of Nitrate Reduction in a Mutant of the Blue-Green Alga Agmenellum quadruplicatum

Synchronous Cultures of Algae

Culture of Chlorella ellipsoidea in different culture media

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