The chemical profiling of aqueous soluble fraction from Lagopsis supina and its diuretic effects via suppression of AQP and RAAS pathways in saline-loaded rats

Yang, Li; He, Zhongwei; He, Junwei

doi:10.1016/j.jep.2021.113951

Cited by 16 publications

(20 citation statements)

References 27 publications

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“…In the present study, CW remarkably decreased serum AQP1, AQP2, and AQP3 excretions and suppressed the mRNA and protein expressions of AQP1, AQP2, and AQP3 compared with the CON group in rats. This indicated that CW exhibited diuretic activity by regulating AQP1, AQP2, and AQP3 expressions in rats, which was in accordance with the previous studies ( 8 , 9 ). These studies observed that flavonoids and/or phenylpropanoids from Lagopsis supine , Bauhinia forficata Link Leaves, and Clerodendrum myricoides serve as the key compounds that exhibit diuretic effects via the above mechanisms.…”

Section: Discussionsupporting

confidence: 92%

“…In the present study, CW observably decreased ADH, ALD, and Ang II levels, while it significantly increased the ANP level compared to the CON group, which eventually suppressed RAAS activation. In some previous studies, the ethanol extract of Lagopsis supine was also observed to exert a diuretic effect via a similar mechanism (9,33).…”

Section: Discussionmentioning

confidence: 86%

“…The phytochemical profiles of coconut water, especially for the polyphenols compounds composition, were determined by using UPLC–Q-TOF–MS/MS including Shimadzu UHPLC System (Kyoto, Japan) tandem with an AB SCIEX 5600 system (Foster City, CA, United States) according to a previous study ( 9 ).…”

Section: Methodsmentioning

confidence: 99%

“…The experiment was performed according to Yang et al ( 9 ) with slight modification as follows: 36 rats were given 5 mL/100 g of normal saline to apply the same level of saline load before treatment. They were randomly divided into four groups ( n = 9 per group), which were treated by gavage with 10 mL/kg⋅bw of normal saline (CON group), 10 mL/kg⋅bw furosemide of 10 mg/kg (F-10 group), coconut water (CW-L group), and concentrated coconut water (CW-H group).…”

Section: Methodsmentioning

confidence: 99%

“…At present, little study has already been done previously to determine the diuretic effect of coconut water, and the underlying mechanism remains unclear. In some previous studies, the glycosides and flavonoids extracted from plant Lagopsis supina were observed to exhibit a significant diuretic effect via suppression of aquaporins (AQPs) and renin–angiotensin–aldosterone system (RAAS) pathways in saline-loaded rats ( 9 ). Therefore, the current study aimed to investigate the acute and prolonged diuretic effect of coconut water and underlying mechanisms in the saline-loaded rat model after characterization of phytochemical constituents in coconut water by ultrahigh-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS).…”

Section: Introductionmentioning

confidence: 99%

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The Diuretic Effects of Coconut Water by Suppressing Aquaporin and Renin–Angiotensin–Aldosterone System in Saline-Loaded Rats

et al. 2022

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The acute and prolonged diuretic effects of coconut water (CW) and the underlying mechanism were investigated with a saline-loaded rat model. In an acute diuretic experiment, CW could significantly increase urine excretion. In addition, the treatment of CW significantly increased urinary sodium and chloride ions, thereby considerably increasing the excretion of NaCl. However, the calcium concentration and pH value were not affected. In the prolonged diuretic experiment, CW dramatically increased the urine output and urine electrolyte concentrations (Na+, K+, and Cl–). Furthermore, CW could suppress the activation of renin–angiotensin–aldosterone system by decreasing serum antidiuretic hormone, angiotensin II, and aldosterone levels, and significantly increasing the serum atriopeptin level. CW treatment significantly reduced the mRNA expressions and protein levels of aquaporin 1 (AQP1), AQP2, and AQP 3. This report provided basic data for explaining the natural tropical beverage of CW as an alternative diuretic agent.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Diuretic Effects of Coconut Water by Suppressing Aquaporin and Renin–Angiotensin–Aldosterone System in Saline-Loaded Rats

et al. 2022

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Revealing the Active Constituents and Mechanisms of Semiliquidambar cathayensis Chang Roots against Rheumatoid Arthritis through Network Pharmacology, Molecular Docking, and in Vivo Experiment

Xia

Zeng

Wang

et al. 2022

Chemistry & Biodiversity

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Semiliquidambar cathayensis Chang roots (SC) are traditional Chinese medicine for treating rheumatoid arthritis (RA). However, the effect and potential mechanism of SC remain unclear. This study aims to reveal the anti-RA constituents and mechanisms of SC based on network pharmacology, molecular docking, and adjuvantinduced arthritis (AIA) model rat experiment. In this work, 9 potential active constituents, including kaempferol, quercetin, naringenin, paeoniflorin, catechin, fraxin, gentianin, hesperetin, and ellagic acid 3,3',4-trimethyl ether, in SC crossed 65 target genes of RA. In addition, 28 core targets were enriched in inflammation and others, among which interleukin-17 (IL-17) and tumor necrosis factor (TNF) were the major targets. The binding of bioconstituents with IL-17 and TNF were performed using molecular docking. Rat experiment demonstrated that the extract of SC restored body weight loss, reduced arthritis score and the indices of thymus and spleen, alleviated ankle joint histopathology, decreased the levels of rheumatoid factor (RF), C-reactive protein (CRP), IL-17, TNF-α, IL-1β, IL-6, cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LOX), and matrix metalloproteinase-2 (MMP-2), whereas elevated the levels of IL-4 and IL-10. Collectively, it was the first time to comprehensively reveal the anti-RA efficacy and mechanism of SC via suppressing the inflammatory pathway based on network pharmacology, molecular docking, and experimental verification, which provide chemical and pharmacological evidences for the clinical application of SC.

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Activating Blood Circulation, Anti‐Inflammatory and Diuretic Effects of Leonurus japonicus Extract on a Rat Model of Trauma Blood Stasis and Its Phytochemical Profiling

Zhang

Nie

Yang

et al. 2023

Chemistry & Biodiversity

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Leonurus japonicus Houtt. has been traditionally used to treat many ailments. This study evaluated the activating blood circulation, anti-inflammatory, and diuretic effects of L. japonicus extract (LJ) and identified its phytochemicals. In this work, the phytochemicals in LJ were identified using liquid chromatography mass spectrometry. Rats were randomly assigned to three groups (n = 8): Control group was treated with saline, while the Model group (saline) and LJ group (426 mg/kg) had induced traumatic injury. All rats were treated with once by daily oral gavage for one week. The biochemical indices and protein expression were measured. Herein, 79 constituents were identified in LJ, which were effective in elevating body weight, food consumption, water intake, and urinary excretion volume, as well as in ameliorating traumatic muscle tissues in model rats. In addition, LJ prominently decreased the contents of plasma viscosity, platelet aggregation rate, thrombin time, prothrombin time, activated partial thromboplastin time, fibrinogen, thromboxane B2 (TXB2), TXB2/6-ketoprostaglandin F1α (6-keto-PGF1α), urokinase-type plasminogen activator (u-PA), plasminogen activator inhibitor 1 (PAI-1), PAI-1/tissue-type PA (t-PA), and PAI-1/u-PA, while significantly increasing antithrombin III, 6-keto-PGF1α, and t-PA contents. Furthermore, LJ notably inhibited tumor necrosis factor alpha, interleukin 6 (IL-6), IL-8, angiotensin II, antidiuretic hormone, aldosterone, aquaporin 1 (AQP1), AQP2, and AQP3 levels, and markedly elevating IL-10 and natriuretic peptide levels. Finally, LJ markedly reduced the protein expression of AQP1, AQP2, and AQP3 compared to the model group. Collectively, LJ possessed prominent activating blood circulation, antiinflammatory, and diuretic effects, thus supporting the clinical application of L. japonicus.

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The chemical profiling of aqueous soluble fraction from Lagopsis supina and its diuretic effects via suppression of AQP and RAAS pathways in saline-loaded rats

Cited by 16 publications

References 27 publications

The Diuretic Effects of Coconut Water by Suppressing Aquaporin and Renin–Angiotensin–Aldosterone System in Saline-Loaded Rats

The Diuretic Effects of Coconut Water by Suppressing Aquaporin and Renin–Angiotensin–Aldosterone System in Saline-Loaded Rats

Revealing the Active Constituents and Mechanisms of Semiliquidambar cathayensis Chang Roots against Rheumatoid Arthritis through Network Pharmacology, Molecular Docking, and in Vivo Experiment

Activating Blood Circulation, Anti‐Inflammatory and Diuretic Effects of Leonurus japonicus Extract on a Rat Model of Trauma Blood Stasis and Its Phytochemical Profiling

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