Organic farming is often seen as a sustainable alternative to intensive agricultural systems. The studies conducted in this direction analyze various factors, as well as their assemblies, and show contradictory results. In order to assess the impact of the organic method of soil cultivation on the stable composition of the most important mineral and organic substances in the production process, the organic agriculture procedure was implemented with an agrochemical analysis for 12 years. The content of mobile phosphorus, exchangeable potassium, and humus in the soil was determined. An elemental analysis of soil samples was conducted for a more in-depth analysis of its composition. It was established that the soils of the farm contained a sufficient amount of exchangeable potassium and humus. The content of these components remained stable during the study period. It was discovered that the soils of the farm have a low content of mobile phosphorus, which also remained stable during the study period. In the studied farm, the applied farming technologies contribute to the stable content of the main nutrient components of the soil. But to correct the content of mobile forms of phosphorus, additional agrotechnical measures are required.