The Chemiluminescent Response from Human Monocyte-Derived Macrophages Exposed to Various Mineral Fibers of Different Sizes.

Abstract

The aims of the present work were to quantify ability to induce lucigenin-dependent chemiluminescence (CL) from 6-9 day-old human monocyte-derived macrophages exposed to various mineral fibers, and to examine the relationship between ability to induce CL and fiber size. All fiber samples induced the CL response from the cells. The relationship between the number of fibers administered and the CL response was examined on all fiber samples by linear regression. The slope of the regression line supplies an approximation of the ability to induce CL. A strong increased correlation between geometric-mean length of fibers and ability to induce CL was observed for the seven fiber samples more than 6 µm in length (r=0.9895). Geometric-mean width and the ability to induce CL showed no correlation. However, among the two fiber samples having a similar length distribution (RF2, RF3), the wider width sample (RF3, 2.4 µm) demonstrated lower ability to induce the CL than the narrower width sample (RF2, 1.1 µm). The present method enabled comparison of ability to induce lucigenin-dependent CL from human monocyte-derived macrophages for various mineral fibers with different sizes. Our findings suggested the possibility that ability to induce O 2 -production increased with fiber length, when fibers are longer than approximately 6 µm.

Comparing the role of silica particle size with mineral fiber geometry in the release of superoxide from rat alveolar macrophages

“…We previously suggested that macrophages non-specifically induce the release of superoxide on exposure to different types of mineral fibers, depending on fiber length; moreover, the increase rate of the ability to induce chemiluminescence (CL) depends on fiber width. We demonstrated a strong correlation between geometric-mean fiber length and the ability to induce CL (largest r 2 = 0.9760) and a negative correlation between geometric-mean fiber width and the increase rate of the ability to induce CL (largest r 2 = 0.7473) in seven types of mineral fibers (Ohyama et al, 2000(Ohyama et al, , 2001. Therefore, we consider that the reactivity of the superoxide response from macrophages not only depends on fiber geometry but also on particle size.…”

Faserstäube, anorganisch [MAK Value Documentation in German language, 2018]

“…Untersuchungen in vitro in Abwesenheit und Anwesenheit von BEAS2BZellen haben gezeigt, dass lange nadelförmige MWCNT (Durchmesser = 74 nm, Län-ge = 5,7 µm; Durchmesser = 64 nm, Länge = 4 µm) zu einer dosisabhängigen Bildung von bisher nicht identifizierten Radikalen führten (Nymark et al 2014). (Ohyama et al 2000(Ohyama et al , 2001. Bei der Entstehung reaktiver Sauerstoffspezies ist auch die membranständige NADPHOxidase beteiligt, die das Primärprodukt O 2…”

Effect of size of man-made and natural mineral fibers on chemiluminescent response in human monocyte-derived macrophages.

“…We demonstrated previously a method for comparing the ability to induce lucigenin-dependent chemiluminescence (CL) per fiber from human monocyte-derived macrophages exposed to nine types of mineral fibers of different sizes at the acute phase of the response (20). We observed that the ability to induce CL increased with fiber length at the acute phase of the response, when the mineral fibers were longer than approximately 6 µm.…”

“…We analyzed the ability to induce CL per fiber of each sample as described previously (20). Briefly, we examined the relation between the estimated number of fibers administered and CL response by linear regression.…”

“…Among various mineral particles, fibrous dust causes a significant increase in the release of superoxide from macrophages (11,17), whereas nonfibrous particles were less active in this regard (18). However, only a few studies have examined in detail the relationship between length and release of superoxide with manmade mineral fibers (19,20). Moreover, it was suggested that fiber length is not an important factor in the ability of man-made mineral fibers to induce production of reactive oxygen species in polymorphonuclear leukocytes (19).…”